##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Alw44I AocI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BanII BbrPI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaI BseAI
BsiEI BsiHKAI BsiWI BsmI BsoBI
BspDI BspEI BspMI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FseI FspI HgaI
HincII HindII HpaI KpnI KspI
MaeI MluI MroI MspA1I NaeI
NcoI NgoMIV NheI NotI NruI
NspV PaeR7I PflFI PflMI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 1744 CGCG CG^CG
AcyI 42 GGCGCC G[GA]^CG[CT]C
AflIII 331 ACATGT A^C[GA][CT]GT
AgeI 1155 ACCGGT A^CCGGT
Aor51HI 141 AGCGCT AGC^GCT
BalI 177 TGGCCA TGG^CCA
BamHI 1763 GGATCC G^GATCC
BanI 42 GGCGCC G^G[CT][GA]CC
BbeI 42 GGCGCC GGCGC^C
BbsI 715 GTCTTC GAAGAC(2/6)
BclI 498 TGATCA T^GATCA
BmyI 1531 GTGCCC G[AGT]GC[ACT]^C
BpmI 1629 CTGGAG CTGGAG(16/14)
BpuAI 715 GTCTTC GAAGAC(2/6)
BsaBI 1385 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 42 GGCGCC G[GA]^CG[CT]C
BsgI 1380 GTGCAG GTGCAG(16/14)
BsmBI 31 GAGACG CGTCTC(1/5)
Bsp1286I 1531 GTGCCC G[AGT]GC[ACT]^C
BspLU11I 331 ACATGT A^CATGT
BsrDI 464 GCAATG GCAATG(2/0)
BsrFI 1155 ACCGGT [GA]^CCGG[CT]
BstUI 1744 CGCG CG^CG
BstXI 1790 CCAACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 91 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 1155 ACCGGT [GA]^CCGG[CT]
DraI 2082 TTTAAA TTT^AAA
DraIII 1100 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 141 AGCGCT AGC^GCT
EcoT14I 1796 CCTTGG C^C[AT][AT]GG
EcoT22I 260 ATGCAT ATGCA^T
FauI 1745 GCGGG CCCGC(4/6)
FbaI 498 TGATCA T^GATCA
Hin1I 42 GGCGCC G[GA]^CG[CT]C
KasI 42 GGCGCC G^GCGCC
MamI 1385 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1202 CAATTG C^AATTG
MluNI 177 TGGCCA TGG^CCA
MscI 177 TGGCCA TGG^CCA
MunI 1202 CAATTG C^AATTG
MvnI 1744 CGCG CG^CG
NarI 42 GGCGCC GG^CGCC
NdeI 1666 CATATG CA^TATG
NsiI 260 ATGCAT ATGCA^T
PacI 1070 TTAATTAA TTAAT^TAA
PinAI 1155 ACCGGT A^CCGGT
SapI 137 GAAGAGC GCTCTTC(1/4)
SfoI 42 GGCGCC GGC^GCC
SmlI 1443 CTCAAG C^T[CT][GA]AG
StyI 1796 CCTTGG C^C[AT][AT]GG
TaqI 1393 TCGA T^CGA
TthHB8I 1393 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AseI 1069 ATTAAT AT^TAAT
1073 ATTAAT
AsnI 1069 ATTAAT AT^TAAT
1073 ATTAAT
BcnI 46 CCGGG CC^[GC]GG
52 CCCGG
BsaWI 61 ACCGGA [AT]^CCGG[AT]
1155 ACCGGT
BsmFI 48 GGGAC GGGAC(10/14)
112 GTCCC
Bsp1407I 1316 TGTACA T^GTACA
1852 TGTACA
BsrGI 1316 TGTACA T^GTACA
1852 TGTACA
DraII 48 GGGACCC [GA]G^G[ACGT]CC[CT]
1453 AGGACCT
EcoO109I 48 GGGACCC [GA]G^G[ACGT]CC[CT]
1453 AGGACCT
HaeII 42 GGCGCC [GA]GCGC^[CT]
141 AGCGCT
HindIII 1263 AAGCTT A^AGCTT
2006 AAGCTT
MwoI 170 GCAACACTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1439 GCTACTCAAGC
NciI 46 CCGGG CC^[GC]GG
52 CCCGG
PleI 210 GAGTC GAGTC(4/5)
945 GAGTC
PpuMI 48 GGGACCC [GA]G^G[AT]CC[CT]
1453 AGGACCT
PshBI 1069 ATTAAT AT^TAAT
1073 ATTAAT
SfcI 1032 CTGTAG C^T[GA][CT]AG
1251 CTATAG
SspBI 1316 TGTACA T^GTACA
1852 TGTACA
Tsp45I 390 GTCAC ^GT[GC]AC
1832 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BbvI BsaJI
BseRI BsiYI BslI BsmAI BspHI
BsrI BssKI BstF5I BstNI BstYI
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI NdeII NlaIII
NlaIV NspI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SspI TfiI
Tru9I TseI Tsp509I TspRI XhoII