##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbeI
BbsI BciVI BclI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DrdI Eam1105I
Eco47III Eco81I EcoRV FbaI FseI
FspI HindIII KasI KpnI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I RcaI RsrII SacI
SalI SbfI ScaI SexAI SfoI
SfuI SgrAI SpeI Sse8387I SspBI
SspI SwaI Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 605 GACGCC G[GA]^CG[CT]C
AflII 291 CTTAAG C^TTAAG
Alw44I 316 GTGCAC G^TGCAC
ApaLI 316 GTGCAC G^TGCAC
Asp700I 669 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 522 GGATCC G^GATCC
BanII 123 GAGCCC G[GA]GC[CT]^C
BbrPI 1450 CACGTG CAC^GTG
BbvCI 2141 CCTCAGC CCTCAGC(-5/-2)
BcgI 176 CGACATAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 291 CTTAAG C^TTAAG
BsaHI 605 GACGCC G[GA]^CG[CT]C
BspMI 1129 ACCTGC ACCTGC(4/8)
BsrBI 1399 GAGCGG CCGCTC(-3/-3)
BsrDI 350 CATTGC GCAATG(2/0)
BssSI 1361 CACGAG CACGAG(-5/-1)
BstAPI 1426 GCAGTGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2092 GGTCACC G^GT[ACGT]ACC
BstPI 2092 GGTCACC G^GT[ACGT]ACC
DraIII 337 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1999 CGGCCG C^GGCCG
EclXI 1999 CGGCCG C^GGCCG
Eco52I 1999 CGGCCG C^GGCCG
EcoNI 1667 CCTAGTATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2092 GGTCACC G^GT[ACGT]ACC
Hin1I 605 GACGCC G[GA]^CG[CT]C
HpaI 844 GTTAAC GTT^AAC
KspI 71 CCGCGG CCGC^GG
NaeI 2001 GCCGGC GCC^GGC
NgoMIV 2001 GCCGGC G^CCGGC
NruI 601 TCGCGA TCG^CGA
PmaCI 1450 CACGTG CAC^GTG
PmlI 1450 CACGTG CAC^GTG
SacII 71 CCGCGG CCGC^GG
SapI 1396 GAAGAGC GCTCTTC(1/4)
SfiI 2048 GGCCAGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 757 CCCGGG CCC^GGG
SmlI 291 CTTAAG C^T[CT][GA]AG
SnaBI 141 TACGTA TAC^GTA
SphI 1439 GCATGC GCATG^C
XbaI 1734 TCTAGA T^CTAGA
XmaCI 757 CCCGGG C^CCGGG
XmaI 757 CCCGGG C^CCGGG
XmnI 669 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 72 CGCG CG^CG
602 CGCG
AflIII 1449 ACACGT A^C[GA][CT]GT
1451 ACGTGT
AspHI 316 GTGCAC G[AT]GC[AT]^C
562 GAGCAC
AvaI 757 CCCGGG C^[CT]CG[GA]G
1330 CCCGAG
BalI 86 TGGCCA TGG^CCA
1528 TGGCCA
BcnI 757 CCCGG CC^[GC]GG
758 CCGGG
BglI 1504 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2049 GCCAGGGTGGC
BpmI 579 CTCCAG CTGGAG(16/14)
1088 CTCCAG
BsaAI 141 TACGTA [CT]AC^GT[GA]
1450 CACGTG
BsaI 569 GAGACC GGTCTC(1/5)
1199 GAGACC
BseRI 803 CTCCTC GAGGAG(10/8)
995 GAGGAG
BsiHKAI 316 GTGCAC G[AT]GC[AT]^C
562 GAGCAC
BsmI 1431 GAATGC GAATGC(1/-1)
1443 GCATTC
BsoBI 757 CCCGGG C^[CT]CG[GA]G
1330 CCCGAG
BsrFI 466 ACCGGC [GA]^CCGG[CT]
2001 GCCGGC
BstUI 72 CGCG CG^CG
602 CGCG
Cfr10I 466 ACCGGC [GA]^CCGG[CT]
2001 GCCGGC
EcoRI 270 GAATTC G^AATTC
2379 GAATTC
FauI 70 CCCGC CCCGC(4/6)
1534 GCGGG
HaeII 1559 GGCGCT [GA]GCGC^[CT]
1610 GGCGCT
HincII 844 GTTAAC GT[CT]^[GA]AC
2356 GTTGAC
HindII 844 GTTAAC GT[CT]^[GA]AC
2356 GTTGAC
MluNI 86 TGGCCA TGG^CCA
1528 TGGCCA
MscI 86 TGGCCA TGG^CCA
1528 TGGCCA
MslI 1448 CACACGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1450 CACGTGTGTG
MvnI 72 CGCG CG^CG
602 CGCG
NciI 757 CCCGG CC^[GC]GG
758 CCGGG
NcoI 89 CCATGG C^CATGG
484 CCATGG
PpuMI 66 GGGTCCC [GA]G^G[AT]CC[CT]
1281 GGGTCCT
PvuI 32 CGATCG CGAT^CG
329 CGATCG
StuI 657 AGGCCT AGG^CCT
2138 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BbvI BfaI
BmyI BsaJI BsgI BsiEI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII