##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AatII       AccIII      AflIII      AgeI        AhdI        
Aor51HI     AscI        AseI        AsnI        AspEI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BglI        BseAI       BsiEI       BsiWI       BspDI       
BspEI       BspLU11I    BssHII      BssSI       BstAPI      
BstBI       BstEII      BstPI       ClaI        CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRV       
FseI        HaeII       HpaI        KasI        KspI        
MfeI        MluI        MroI        MunI        NarI        
NotI        NruI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       PvuI        RsrII       SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       Sse8387I    
SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I       1865  GGTACC               G^GTACC
AccII        1156  CGCG                 CG^CG
AclI         2744  AACGTT               AA^CGTT
AcyI          935  GACGCC               G[GA]^CG[CT]C
AflII        2884  CTTAAG               C^TTAAG
Alw44I       3183  GTGCAC               G^TGCAC
AlwNI        3572  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2246  CCTAAGG              CC^T[ACGT]AGG
ApaLI        3183  GTGCAC               G^TGCAC
Asp700I       771  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1865  GGTACC               G^GTACC
AspI         1190  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2264  TGCGCA               TGC^GCA
BamHI        1008  GGATCC               G^GATCC
BcnI         1603  CCGGG                CC^[GC]GG
BfrI         2884  CTTAAG               C^TTAAG
BsaAI        2053  TACGTG               [CT]AC^GT[GA]
BsaHI         935  GACGCC               G[GA]^CG[CT]C
BsaWI        1584  ACCGGA               [AT]^CCGG[AT]
Bsp1407I     3478  TGTACA               T^GTACA
BspHI        1950  TCATGA               T^CATGA
BsrBI         546  CCGCTC               CCGCTC(-3/-3)
BsrGI        3478  TGTACA               T^GTACA
BstUI        1156  CGCG                 CG^CG
Bsu36I       2246  CCTAAGG              CC^T[ACGT]AGG
Eco81I       2246  CCTAAGG              CC^T[ACGT]AGG
EcoRI        2660  GAATTC               G^AATTC
FspI         2264  TGCGCA               TGC^GCA
Hin1I         935  GACGCC               G[GA]^CG[CT]C
HindIII      1294  AAGCTT               A^AGCTT
KpnI         1865  GGTACC               GGTAC^C
MvnI         1156  CGCG                 CG^CG
NciI         1603  CCGGG                CC^[GC]GG
NcoI         2801  CCATGG               C^CATGG
NdeI         4214  CATATG               CA^TATG
PflFI        1190  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2006  CCACAGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I     2744  AACGTT               AA^CGTT
RcaI         1950  TCATGA               T^CATGA
SacI         1928  GAGCTC               GAGCT^C
ScaI         2968  AGTACT               AGT^ACT
SpeI          208  ACTAGT               A^CTAGT
SphI         2467  GCATGC               GCATG^C
SspBI        3478  TGTACA               T^GTACA
Tth111I      1190  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2006  CCACAGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          771  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI         3846  GTATAC               GT^[AC][GT]AC
             4346  GTATAC              
ApaI         1718  GGGCCC               GGGCC^C
             3656  GGGCCC              
AvrII        2694  CCTAGG               C^CTAGG
             3903  CCTAGG              
BclI          681  TGATCA               T^GATCA
             2543  TGATCA              
BglII        1046  AGATCT               A^GATCT
             1467  AGATCT              
BlnI         2694  CCTAGG               C^CTAGG
             3903  CCTAGG              
BlpI         3301  GCTCAGC              GC^T[ACGT]AGC
             3618  GCTTAGC             
Bpu1102I     3301  GCTCAGC              GC^T[ACGT]AGC
             3618  GCTTAGC             
BsaI         1628  GAGACC               GGTCTC(1/5)
             4694  GAGACC              
BsmBI         933  GAGACG               CGTCTC(1/5)
             3707  GAGACG              
BsmI         2083  GAATGC               GAATGC(1/-1)
             3518  GCATTC              
BsrDI        3759  CATTGC               GCAATG(2/0)
             1764  GCAATG              
Bst1107I     3846  GTATAC               GTA^TAC
             4346  GTATAC              
BstZ17I      3846  GTATAC               GTA^TAC
             4346  GTATAC              
CelII        3301  GCTCAGC              GC^T[ACGT]AGC
             3618  GCTTAGC             
DraI         2388  TTTAAA               TTT^AAA
             4650  TTTAAA              
DsaI           87  CCGTGG               C^C[GA][CT]GG
             2801  CCATGG              
EcoT22I       199  ATGCAT               ATGCA^T
             2452  ATGCAT              
FbaI          681  TGATCA               T^GATCA
             2543  TGATCA              
HgaI          935  GACGC                GACGC(5/10)
             3709  GACGC               
NaeI         1653  GCCGGC               GCC^GGC
             1883  GCCGGC              
NgoMIV       1653  GCCGGC               G^CCGGC
             1883  GCCGGC              
NsiI          199  ATGCAT               ATGCA^T
             2452  ATGCAT              
SspI         4472  AATATT               AAT^ATT
             4568  AATATT              
StuI         1219  AGGCCT               AGG^CCT
             2761  AGGCCT              
XbaI          277  TCTAGA               T^CTAGA
             3897  TCTAGA              
XcmI         1770  CCAGTGGAGGGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2145  CCACCACTGAATTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BfaI        
BmyI        BpmI        BpuAI       BsaBI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NheI        NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII