##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AhdI        AscI        AseI        AsnI        Asp700I     
AspEI       AvrII       BciVI       BclI        BfrI        
BglII       BlnI        BsaBI       BseAI       BseRI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BsrGI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        Eam1105I    EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HincII      HindII      HindIII     HpaI        
MamI        MfeI        MluI        MroI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        SalI        
SexAI       SfiI        SfuI        SnaBI       SpeI        
SphI        SspBI       SspI        SwaI        XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AflIII       2815  ACATGT               A^C[GA][CT]GT
AgeI         1981  ACCGGT               A^CCGGT
Alw44I        710  GTGCAC               G^TGCAC
Aor51HI       982  AGCGCT               AGC^GCT
ApaLI         710  GTGCAC               G^TGCAC
AspI         1107  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcgI         2071  CGATCTACATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI        1981  ACCGGT               [AT]^CCGG[AT]
BspLU11I     2815  ACATGT               A^CATGT
BsrDI        3345  GCAATG               GCAATG(2/0)
BssSI        3079  CTCGTG               CACGAG(-5/-1)
BstAPI       2923  GCAAGCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI         2329  CGGTCCG              CG^G[AT]CCG
DraI         3155  TTTAAA               TTT^AAA
DraIII        771  CACGGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III      982  AGCGCT               AGC^GCT
PflFI        1107  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1981  ACCGGT               A^CCGGT
RsrII        2329  CGGTCCG              CG^G[AT]CCG
SapI         1301  GCTCTTC              GCTCTTC(1/4)
SbfI         2714  CCTGCAGG             CCTGCA^GG
SfaNI         229  GATGC                GCATC(5/9)
SgrAI        1980  CACCGGTG             C[GA]^CCGG[CT]G
SmlI         1356  CTCAAG               C^T[CT][GA]AG
Sse8387I     2714  CCTGCAGG             CCTGCA^GG
Tth111I      1107  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

Acc65I       1035  GGTACC               G^GTACC
             1785  GGTACC              
AocI         1701  CCTGAGG              CC^T[ACGT]AGG
             1881  CCTGAGG             
Asp718I      1035  GGTACC               G^GTACC
             1785  GGTACC              
AviII         126  TGCGCA               TGC^GCA
              783  TGCGCA              
BamHI        2100  GGATCC               G^GATCC
             2408  GGATCC              
BbeI          531  GGCGCC               GGCGC^C
              562  GGCGCC              
BbvCI        1457  CCTCAGC              CCTCAGC(-5/-2)
             2959  CCTCAGC             
BlpI          111  GCTGAGC              GC^T[ACGT]AGC
             2013  GCTGAGC             
Bpu1102I      111  GCTGAGC              GC^T[ACGT]AGC
             2013  GCTGAGC             
BsaI         2682  GAGACC               GGTCTC(1/5)
             1769  GGTCTC              
BsgI          777  GTGCAG               GTGCAG(16/14)
             2897  GTGCAG              
BsrBI        1836  CCGCTC               CCGCTC(-3/-3)
             2117  CCGCTC              
BssHII        510  GCGCGC               G^CGCGC
              738  GCGCGC              
Bsu36I       1701  CCTGAGG              CC^T[ACGT]AGG
             1881  CCTGAGG             
CelII         111  GCTGAGC              GC^T[ACGT]AGC
             2013  GCTGAGC             
DrdI           50  GACTCAGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1500  GACCCGGAGGTC        
EagI          617  CGGCCG               C^GGCCG
              633  CGGCCG              
EclXI         617  CGGCCG               C^GGCCG
              633  CGGCCG              
Eco52I        617  CGGCCG               C^GGCCG
              633  CGGCCG              
Eco81I       1701  CCTGAGG              CC^T[ACGT]AGG
             1881  CCTGAGG             
FspI          126  TGCGCA               TGC^GCA
              783  TGCGCA              
KasI          531  GGCGCC               G^GCGCC
              562  GGCGCC              
KpnI         1035  GGTACC               GGTAC^C
             1785  GGTACC              
MslI           84  CACGTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              789  CACAGCTGTG          
NarI          531  GGCGCC               GG^CGCC
              562  GGCGCC              
NcoI            1  CCATGG               C^CATGG
             1169  CCATGG              
NspI         2077  ACATGC               [GA]CATG^[CT]
             2815  ACATGT              
SacI         1824  GAGCTC               GAGCT^C
             2051  GAGCTC              
ScaI          280  AGTACT               AGT^ACT
             3372  AGTACT              
SfoI          531  GGCGCC               GGC^GCC
              562  GGCGCC              
StuI          952  AGGCCT               AGG^CCT
             2671  AGGCCT              
TaqI          227  TCGA                 T^CGA
             1531  TCGA                
TthHB8I       227  TCGA                 T^CGA
             1531  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbrPI       BbsI        BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaAI       BsaHI       BsaJI       BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       PflMI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        SacII       
Sau3AI      Sau96I      ScrFI       SfcI        SmaI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Van91I      XcmI        XhoII       
XmaCI       XmaI