##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       AclI        AflII       AhdI        Alw44I      
ApaLI       AscI        AspEI       AspI        AvrII       
BamHI       BfrI        BglI        BlnI        BsiEI       
BsiWI       BsmI        BspDI       BspMI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        EagI        
Eam1105I    EclXI       Eco52I      EcoO65I     EcoRV       
FseI        HpaI        KspI        MluI        NaeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PmeI        Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
SexAI       SfiI        SfuI        SnaBI       SpeI        
Sse8387I    SspI        StuI        SwaI        Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I       1667  GGTACC               G^GTACC
AccII        1118  CGCG                 CG^CG
AccIII       2720  TCCGGA               T^CCGGA
AcyI         1390  GGCGCC               G[GA]^CG[CT]C
AgeI         1420  ACCGGT               A^CCGGT
Aor51HI       536  AGCGCT               AGC^GCT
Asp700I      2404  GAATTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1667  GGTACC               G^GTACC
BbeI         1390  GGCGCC               GGCGC^C
BbrPI        2626  CACGTG               CAC^GTG
BbvCI         754  GCTGAGG              CCTCAGC(-5/-2)
BcgI         2909  GCAAATTCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI          63  GGATAC               GTATCC(6/5)
BsaHI        1390  GGCGCC               G[GA]^CG[CT]C
BseAI        2720  TCCGGA               T^CCGGA
BspEI        2720  TCCGGA               T^CCGGA
BsrBI         778  GAGCGG               CCGCTC(-3/-3)
BsrDI        1769  GCAATG               GCAATG(2/0)
BstUI        1118  CGCG                 CG^CG
DrdI         2029  GACCCAGTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      536  AGCGCT               AGC^GCT
EcoRI        2854  GAATTC               G^AATTC
EcoT22I      1981  ATGCAT               ATGCA^T
Hin1I        1390  GGCGCC               G[GA]^CG[CT]C
HindIII      2675  AAGCTT               A^AGCTT
KasI         1390  GGCGCC               G^GCGCC
KpnI         1667  GGTACC               GGTAC^C
MfeI         1733  CAATTG               C^AATTG
MroI         2720  TCCGGA               T^CCGGA
MunI         1733  CAATTG               C^AATTG
MvnI         1118  CGCG                 CG^CG
NarI         1390  GGCGCC               GG^CGCC
NdeI         3049  CATATG               CA^TATG
NsiI         1981  ATGCAT               ATGCA^T
PflMI         897  CCACATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1420  ACCGGT               A^CCGGT
PmaCI        2626  CACGTG               CAC^GTG
PmlI         2626  CACGTG               CAC^GTG
SapI         2329  GCTCTTC              GCTCTTC(1/4)
ScaI          251  AGTACT               AGT^ACT
SfoI         1390  GGCGCC               GGC^GCC
SgrAI        1419  CACCGGTG             C[GA]^CCGG[CT]G
SmaI         2849  CCCGGG               CCC^GGG
SphI          792  GCATGC               GCATG^C
Van91I        897  CCACATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        2849  CCCGGG               C^CCGGG
XmaI         2849  CCCGGG               C^CCGGG
XmnI         2404  GAATTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   44 enzymes) #
###################################

AccI         2506  GTCTAC               GT^[AC][GT]AC
             3610  GTCTAC              
AlwNI         815  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1002  CAGCATCTG           
AocI          283  CCTGAGG              CC^T[ACGT]AGG
             3626  CCTCAGG             
ApaI         2388  GGGCCC               GGGCC^C
             3253  GGGCCC              
AseI          921  ATTAAT               AT^TAAT
             1060  ATTAAT              
AsnI          921  ATTAAT               AT^TAAT
             1060  ATTAAT              
AvaI         1763  CCCGAG               C^[CT]CG[GA]G
             2849  CCCGGG              
AviII         716  TGCGCA               TGC^GCA
             1880  TGCGCA              
BalI         1661  TGGCCA               TGG^CCA
             2609  TGGCCA              
BfaI         1145  CTAG                 C^TAG
             1246  CTAG                
BlpI           41  GCTGAGC              GC^T[ACGT]AGC
             2125  GCTGAGC             
Bpu1102I       41  GCTGAGC              GC^T[ACGT]AGC
             2125  GCTGAGC             
BsaAI        2181  TACGTG               [CT]AC^GT[GA]
             2626  CACGTG              
BsaBI        2010  GATAGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2227  GATTGGGATC          
BsaWI        1420  ACCGGT               [AT]^CCGG[AT]
             2720  TCCGGA              
BsoBI        1763  CCCGAG               C^[CT]CG[GA]G
             2849  CCCGGG              
Bsp1407I     1502  TGTACA               T^GTACA
             2663  TGTACA              
BspHI         201  TCATGA               T^CATGA
             2858  TCATGA              
BsrFI         842  ACCGGC               [GA]^CCGG[CT]
             1420  ACCGGT              
BsrGI        1502  TGTACA               T^GTACA
             2663  TGTACA              
BstXI        1913  CCACCAAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2285  CCAACATTCTGG        
Bsu36I        283  CCTGAGG              CC^T[ACGT]AGG
             3626  CCTCAGG             
CelII          41  GCTGAGC              GC^T[ACGT]AGC
             2125  GCTGAGC             
Cfr10I        842  ACCGGC               [GA]^CCGG[CT]
             1420  ACCGGT              
DraIII        819  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
              898  CACATTGTG           
Eco81I        283  CCTGAGG              CC^T[ACGT]AGG
             3626  CCTCAGG             
FauI         2870  CCCGC                CCCGC(4/6)
             2471  GCGGG               
FspI          716  TGCGCA               TGC^GCA
             1880  TGCGCA              
HgaI         1116  GACGC                GACGC(5/10)
             2585  GCGTC               
HincII       1843  GTCAAC               GT[CT]^[GA]AC
             1888  GTCAAC              
HindII       1843  GTCAAC               GT[CT]^[GA]AC
             1888  GTCAAC              
MaeI         1145  CTAG                 C^TAG
             1246  CTAG                
MamI         2010  GATAGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2227  GATTGGGATC          
MluNI        1661  TGGCCA               TGG^CCA
             2609  TGGCCA              
MscI         1661  TGGCCA               TGG^CCA
             2609  TGGCCA              
NcoI          477  CCATGG               C^CATGG
             3308  CCATGG              
PpuMI        3466  GGGTCCC              [GA]G^G[AT]CC[CT]
             3565  GGGACCT             
PshAI         618  GACAGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3337  GACCTCTGTC          
PshBI         921  ATTAAT               AT^TAAT
             1060  ATTAAT              
PvuII         707  CAGCTG               CAG^CTG
             3443  CAGCTG              
RcaI          201  TCATGA               T^CATGA
             2858  TCATGA              
SmlI         2074  CTCAAG               C^T[CT][GA]AG
             2134  CTTGAG              
SspBI        1502  TGTACA               T^GTACA
             2663  TGTACA              
XcmI         2285  CCAACATTCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3548  CCATGCTGCTTCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AspHI       AvaII       BanI        
BanII       BbsI        BbvI        BclI        BcnI        
BglII       BmyI        BpmI        BpuAI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       Bsp1286I    
BspLU11I    BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FbaI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII