##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        Aor51HI     
AscI        AseI        AsnI        Asp718I     AviII       
AvrII       BbeI        BcgI        BclI        BglI        
BlnI        BlpI        Bpu1102I    BseAI       BsiWI       
BsmBI       BspEI       BspHI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstZ17I     
CelII       CpoI        DraI        EagI        EclXI       
Eco47III    Eco52I      EcoNI       FbaI        FseI        
FspI        HaeII       KasI        KpnI        KspI        
MfeI        MluI        MroI        MslI        MunI        
MvnI        NarI        NotI        NruI        NspV        
PacI        PmeI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       Sse8387I    
SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AatII        3949  GACGTC               GACGT^C
AflII         554  CTTAAG               C^TTAAG
AhdI         4037  GACCCGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2459  GTGCAC               G^TGCAC
ApaI         1756  GGGCCC               GGGCC^C
ApaLI        2459  GTGCAC               G^TGCAC
AspEI        4037  GACCCGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3386  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1136  TGGCCA               TGG^CCA
BanI          732  GGCACC               G^G[CT][GA]CC
BbrPI        1166  CACGTG               CAC^GTG
BfrI          554  CTTAAG               C^TTAAG
BglII        2936  AGATCT               A^GATCT
BsaAI        1166  CACGTG               [CT]AC^GT[GA]
BsaBI         894  GATGGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1980  GGTCTC               GGTCTC(1/5)
BsiEI        3224  CGACCG               CG[GA][CT]^CG
BsmI         3939  GAATGC               GAATGC(1/-1)
Bsp1407I      245  TGTACA               T^GTACA
BspDI         216  ATCGAT               AT^CGAT
BspLU11I     2455  ACATGT               A^CATGT
BsrBI        1752  GAGCGG               CCGCTC(-3/-3)
BsrDI        2552  CATTGC               GCAATG(2/0)
BsrGI         245  TGTACA               T^GTACA
BstEII        672  GGTGACC              G^GT[ACGT]ACC
BstPI         672  GGTGACC              G^GT[ACGT]ACC
CfoI         3655  GCGC                 GCG^C
ClaI          216  ATCGAT               AT^CGAT
DrdI          675  GACCTGATAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     4037  GACCCGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       672  GGTGACC              G^GT[ACGT]ACC
EcoRI         694  GAATTC               G^AATTC
EcoRV        3480  GATATC               GAT^ATC
FauI         1754  GCGGG                CCCGC(4/6)
HhaI         3655  GCGC                 GCG^C
HinP1I       3655  GCGC                 G^CGC
HpaI         1935  GTTAAC               GTT^AAC
MamI          894  GATGGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1136  TGGCCA               TGG^CCA
MscI         1136  TGGCCA               TGG^CCA
NaeI         1027  GCCGGC               GCC^GGC
NdeI         2071  CATATG               CA^TATG
NgoMIV       1027  GCCGGC               G^CCGGC
PaeR7I       1837  CTCGAG               C^TCGAG
PflFI        3386  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2934  CCAGATCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1166  CACGTG               CAC^GTG
PmlI         1166  CACGTG               CAC^GTG
PpuMI        2773  AGGACCC              [GA]G^G[AT]CC[CT]
PstI         1817  CTGCAG               CTGCA^G
SapI          605  GAAGAGC              GCTCTTC(1/4)
ScaI         2641  AGTACT               AGT^ACT
SpeI         3324  ACTAGT               A^CTAGT
SspBI         245  TGTACA               T^GTACA
StuI         3154  AGGCCT               AGG^CCT
Tth111I      3386  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2934  CCAGATCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1837  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcyI         1927  GGCGTC               G[GA]^CG[CT]C
             3949  GACGTC              
AflIII       1338  ACACGT               A^C[GA][CT]GT
             2455  ACATGT              
AgeI         1401  ACCGGT               A^CCGGT
             2467  ACCGGT              
AocI         3163  CCTCAGG              CC^T[ACGT]AGG
             4002  CCTCAGG             
BbvCI        1297  CCTCAGC              CCTCAGC(-5/-2)
             1702  CCTCAGC             
BsaHI        1927  GGCGTC               G[GA]^CG[CT]C
             3949  GACGTC              
BsaWI        1401  ACCGGT               [AT]^CCGG[AT]
             2467  ACCGGT              
BsgI         1306  CTGCAC               GTGCAG(16/14)
             1612  CTGCAC              
BstXI         749  CCAGACAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2747  CCAGGAAGATGG        
Bsu36I       3163  CCTCAGG              CC^T[ACGT]AGG
             4002  CCTCAGG             
EaeI         1029  CGGCCA               [CT]^GGCC[GA]
             1136  TGGCCA              
Eco81I       3163  CCTCAGG              CC^T[ACGT]AGG
             4002  CCTCAGG             
EcoT22I       337  ATGCAT               ATGCA^T
             3330  ATGCAT              
HgaI         1928  GCGTC                GACGC(5/10)
             3554  GCGTC               
Hin1I        1927  GGCGTC               G[GA]^CG[CT]C
             3949  GACGTC              
HindIII      1794  AAGCTT               A^AGCTT
             3723  AAGCTT              
NsiI          337  ATGCAT               ATGCA^T
             3330  ATGCAT              
PinAI        1401  ACCGGT               A^CCGGT
             2467  ACCGGT              
SmaI         2222  CCCGGG               CCC^GGG
             2878  CCCGGG              
SphI          688  GCATGC               GCATG^C
             1744  GCATGC              
XmaCI        2222  CCCGGG               C^CCGGG
             2878  CCCGGG              
XmaI         2222  CCCGGG               C^CCGGG
             2878  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaI        AvaII       BamHI       BanII       BbsI        
BbvI        BciVI       BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EarI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NheI        NlaIII      NlaIV       NspI        
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       XmnI