##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
Alw44I ApaLI ApoI AscI Asp700I
AvrII BamHI BciVI BclI BfrI
BlnI BsaBI BsiWI BsmI BspDI
BspHI BspLU11I BssHII Bst1107I BstBI
BstZ17I ClaI EcoRI EcoT22I FbaI
FseI HindIII HpaI MamI MfeI
MluI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI Psp1406I PvuI RcaI
SalI SbfI SfuI SgrAI SnaBI
Sse8387I SspI SwaI Tsp509I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 3848 GGTACC G^GTACC
AccI 819 GTAGAC GT^[AC][GT]AC
AhdI 1503 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3828 AGCGCT AGC^GCT
AseI 2754 ATTAAT AT^TAAT
AsnI 2754 ATTAAT AT^TAAT
Asp718I 3848 GGTACC G^GTACC
AspEI 1503 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2739 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Bsp1407I 5077 TGTACA T^GTACA
BsrGI 5077 TGTACA T^GTACA
BssSI 2382 CACGAG CACGAG(-5/-1)
CpoI 1215 CGGACCG CG^G[AT]CCG
DraI 4987 TTTAAA TTT^AAA
DraIII 1630 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2787 GACCACGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3976 CGGCCG C^GGCCG
Eam1105I 1503 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3976 CGGCCG C^GGCCG
Eco47III 3828 AGCGCT AGC^GCT
Eco52I 3976 CGGCCG C^GGCCG
EcoNI 4888 CCTCCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 279 GATATC GAT^ATC
HincII 5119 GTTGAC GT[CT]^[GA]AC
HindII 5119 GTTGAC GT[CT]^[GA]AC
KpnI 3848 GGTACC GGTAC^C
NaeI 2409 GCCGGC GCC^GGC
NgoMIV 2409 GCCGGC G^CCGGC
NspI 1894 GCATGC [GA]CATG^[CT]
PflFI 2739 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4690 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2754 ATTAAT AT^TAAT
RsrII 1215 CGGACCG CG^G[AT]CCG
SapI 2175 GAAGAGC GCTCTTC(1/4)
ScaI 4078 AGTACT AGT^ACT
SexAI 457 ACCTGGT A^CC[AT]GGT
SpeI 5065 ACTAGT A^CTAGT
SphI 1894 GCATGC GCATG^C
SspBI 5077 TGTACA T^GTACA
Tth111I 2739 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccIII 3929 TCCGGA T^CCGGA
4427 TCCGGA
AflIII 265 ACGTGT A^C[GA][CT]GT
679 ACGTGT
AviII 184 TGCGCA TGC^GCA
382 TGCGCA
BalI 235 TGGCCA TGG^CCA
3902 TGGCCA
BglII 91 AGATCT A^GATCT
1846 AGATCT
BseAI 3929 TCCGGA T^CCGGA
4427 TCCGGA
BspEI 3929 TCCGGA T^CCGGA
4427 TCCGGA
BstEII 461 GGTGACC G^GT[ACGT]ACC
2468 GGTCACC
BstPI 461 GGTGACC G^GT[ACGT]ACC
2468 GGTCACC
EcoO65I 461 GGTGACC G^GT[ACGT]ACC
2468 GGTCACC
FspI 184 TGCGCA TGC^GCA
382 TGCGCA
KspI 3129 CCGCGG CCGC^GG
3347 CCGCGG
MluNI 235 TGGCCA TGG^CCA
3902 TGGCCA
MroI 3929 TCCGGA T^CCGGA
4427 TCCGGA
MscI 235 TGGCCA TGG^CCA
3902 TGGCCA
MslI 2414 CACTGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4646 CACCCCTGTG
PaeR7I 972 CTCGAG C^TCGAG
1272 CTCGAG
SacII 3129 CCGCGG CCGC^GG
3347 CCGCGG
TfiI 827 GAATC G^A[AT]TC
3882 GAATC
XhoI 972 CTCGAG C^TCGAG
1272 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SfoI SmaI SmlI StuI
StyI TaqI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI