##################################
# NO CUT ENZYMES (   51 enzymes) #
##################################

AclI        AgeI        AhdI        AscI        AspEI       
AvaI        BamHI       BbeI        BbvCI       BcgI        
BciVI       BsaBI       BsiWI       BsoBI       BspDI       
ClaI        CpoI        DrdI        Eam1105I    FseI        
HpaI        KasI        KspI        MamI        MluI        
NarI        NotI        NruI        PacI        PaeR7I      
PinAI       PmeI        Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SwaI        XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Acc65I       1237  GGTACC               G^GTACC
AccIII       2596  TCCGGA               T^CCGGA
Aor51HI      2684  AGCGCT               AGC^GCT
Asp718I      1237  GGTACC               G^GTACC
AviII        5193  TGCGCA               TGC^GCA
BclI         6908  TGATCA               T^GATCA
BglI         7259  GCCCTGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI        2596  TCCGGA               [AT]^CCGG[AT]
BseAI        2596  TCCGGA               T^CCGGA
BsiEI        1382  CGGCCG               CG[GA][CT]^CG
BsmI         5186  GAATGC               GAATGC(1/-1)
BspEI        2596  TCCGGA               T^CCGGA
BspHI        3776  TCATGA               T^CATGA
BssHII       7218  GCGCGC               G^CGCGC
Bst1107I     2523  GTATAC               GTA^TAC
BstZ17I      2523  GTATAC               GTA^TAC
DraIII       3533  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         1382  CGGCCG               C^GGCCG
EclXI        1382  CGGCCG               C^GGCCG
Eco47III     2684  AGCGCT               AGC^GCT
Eco52I       1382  CGGCCG               C^GGCCG
FbaI         6908  TGATCA               T^GATCA
FspI         5193  TGCGCA               TGC^GCA
HgaI         2224  GACGC                GACGC(5/10)
HincII        541  GTCAAC               GT[CT]^[GA]AC
HindII        541  GTCAAC               GT[CT]^[GA]AC
KpnI         1237  GGTACC               GGTAC^C
MroI         2596  TCCGGA               T^CCGGA
NheI         5588  GCTAGC               G^CTAGC
PshAI        4738  GACATGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         4324  CTGCAG               CTGCA^G
RcaI         3776  TCATGA               T^CATGA
SspI         3973  AATATT               AAT^ATT
StuI         1760  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AatII        1717  GACGTC               GACGT^C
             5891  GACGTC              
AccII        7219  CGCG                 CG^CG
             7272  CGCG                
AcyI         1717  GACGTC               G[GA]^CG[CT]C
             5891  GACGTC              
AflII        3869  CTTAAG               C^TTAAG
             5435  CTTAAG              
ApaI           16  GGGCCC               GGGCC^C
             5087  GGGCCC              
BbrPI        3752  CACGTG               CAC^GTG
             4058  CACGTG              
BbsI          944  GAAGAC               GAAGAC(2/6)
             4735  GAAGAC              
BfrI         3869  CTTAAG               C^TTAAG
             5435  CTTAAG              
BglII        1127  AGATCT               A^GATCT
             1677  AGATCT              
BlpI         2367  GCTCAGC              GC^T[ACGT]AGC
             5213  GCTCAGC             
Bpu1102I     2367  GCTCAGC              GC^T[ACGT]AGC
             5213  GCTCAGC             
BpuAI         944  GAAGAC               GAAGAC(2/6)
             4735  GAAGAC              
BsaAI        3752  CACGTG               [CT]AC^GT[GA]
             4058  CACGTG              
BsaHI        1717  GACGTC               G[GA]^CG[CT]C
             5891  GACGTC              
BsaI          505  GAGACC               GGTCTC(1/5)
             4293  GAGACC              
Bsp1407I     1303  TGTACA               T^GTACA
             6983  TGTACA              
BsrGI        1303  TGTACA               T^GTACA
             6983  TGTACA              
BstBI        3356  TTCGAA               TT^CGAA
             3724  TTCGAA              
BstUI        7219  CGCG                 CG^CG
             7272  CGCG                
CelII        2367  GCTCAGC              GC^T[ACGT]AGC
             5213  GCTCAGC             
EcoNI        2117  CCTCAGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3026  CCTAGGACAGG         
EcoRI        3408  GAATTC               G^AATTC
             7161  GAATTC              
Hin1I        1717  GACGTC               G[GA]^CG[CT]C
             5891  GACGTC              
MfeI          461  CAATTG               C^AATTG
              596  CAATTG              
MunI          461  CAATTG               C^AATTG
              596  CAATTG              
MvnI         7219  CGCG                 CG^CG
             7272  CGCG                
NaeI          603  GCCGGC               GCC^GGC
             7264  GCCGGC              
NgoMIV        603  GCCGGC               G^CCGGC
             7264  GCCGGC              
NspV         3356  TTCGAA               TT^CGAA
             3724  TTCGAA              
PmaCI        3752  CACGTG               CAC^GTG
             4058  CACGTG              
PmlI         3752  CACGTG               CAC^GTG
             4058  CACGTG              
PvuII         842  CAGCTG               CAG^CTG
             5120  CAGCTG              
SacI          129  GAGCTC               GAGCT^C
             3046  GAGCTC              
SapI         1734  GAAGAGC              GCTCTTC(1/4)
             5564  GAAGAGC             
SexAI        1025  ACCAGGT              A^CC[AT]GGT
             5146  ACCTGGT             
SfuI         3356  TTCGAA               TT^CGAA
             3724  TTCGAA              
SspBI        1303  TGTACA               T^GTACA
             6983  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  136 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        Alw44I      AlwI        AlwNI       AocI        
ApaLI       ApoI        AseI        AsnI        Asp700I     
AspHI       AspI        AvaII       AvrII       BalI        
BanI        BanII       BbvI        BcnI        BfaI        
BlnI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       Bsp1286I    BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrI        BssKI       
BssSI       BstAPI      BstEII      BstF5I      BstNI       
BstPI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        EarI        
Eco81I      EcoO109I    EcoO65I     EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PflFI       
PflMI       PleI        PpuMI       PshBI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SphI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XcmI        XhoII       
XmnI