##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AgeI AhdI ApaI AscI AspEI
AviII BbeI BglI BsgI BsiWI
Bsp1407I BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Cfr10I CpoI Eam1105I EcoO65I
FseI FspI HpaI KasI KspI
NaeI NarI NdeI NgoMIV NotI
NruI PaeR7I PinAI PmeI PshAI
PvuI RsrII SacII SalI SbfI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SspBI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 228 GACGTC GACGT^C
Acc65I 4605 GGTACC G^GTACC
AccI 350 GTCTAC GT^[AC][GT]AC
AccIII 4075 TCCGGA T^CCGGA
AclI 4290 AACGTT AA^CGTT
AcyI 228 GACGTC G[GA]^CG[CT]C
Alw44I 5858 GTGCAC G^TGCAC
AocI 1995 CCTTAGG CC^T[ACGT]AGG
Aor51HI 2592 AGCGCT AGC^GCT
ApaLI 5858 GTGCAC G^TGCAC
Asp718I 4605 GGTACC G^GTACC
AspI 1437 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2710 GGATCC G^GATCC
BbrPI 6207 CACGTG CAC^GTG
BlpI 917 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 917 GCTAAGC GC^T[ACGT]AGC
BsaAI 6207 CACGTG [CT]AC^GT[GA]
BsaBI 1403 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 228 GACGTC G[GA]^CG[CT]C
BseAI 4075 TCCGGA T^CCGGA
BsiEI 164 CGGCCG CG[GA][CT]^CG
BspDI 3702 ATCGAT AT^CGAT
BspEI 4075 TCCGGA T^CCGGA
BstBI 4925 TTCGAA TT^CGAA
Bsu36I 1995 CCTTAGG CC^T[ACGT]AGG
CelII 917 GCTAAGC GC^T[ACGT]AGC
ClaI 3702 ATCGAT AT^CGAT
EagI 164 CGGCCG C^GGCCG
EclXI 164 CGGCCG C^GGCCG
Eco47III 2592 AGCGCT AGC^GCT
Eco52I 164 CGGCCG C^GGCCG
Eco81I 1995 CCTTAGG CC^T[ACGT]AGG
EcoT22I 6039 ATGCAT ATGCA^T
HgaI 3161 GCGTC GACGC(5/10)
Hin1I 228 GACGTC G[GA]^CG[CT]C
KpnI 4605 GGTACC GGTAC^C
MamI 1403 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1556 CAATTG C^AATTG
MluI 3159 ACGCGT A^CGCGT
MroI 4075 TCCGGA T^CCGGA
MunI 1556 CAATTG C^AATTG
NcoI 489 CCATGG C^CATGG
NheI 3487 GCTAGC G^CTAGC
NsiI 6039 ATGCAT ATGCA^T
NspV 4925 TTCGAA TT^CGAA
PacI 1754 TTAATTAA TTAAT^TAA
PflFI 1437 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 6207 CACGTG CAC^GTG
PmlI 6207 CACGTG CAC^GTG
Psp1406I 4290 AACGTT AA^CGTT
SacI 1469 GAGCTC GAGCT^C
SapI 875 GAAGAGC GCTCTTC(1/4)
SexAI 1710 ACCTGGT A^CC[AT]GGT
SfuI 4925 TTCGAA TT^CGAA
SspI 1586 AATATT AAT^ATT
SwaI 4164 ATTTAAAT ATTT^AAAT
Tth111I 1437 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5701 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflII 3865 CTTAAG C^TTAAG
5966 CTTAAG
AflIII 3159 ACGCGT A^C[GA][CT]GT
6206 ACACGT
Asp700I 370 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5693 GAAGCATTTC
AvrII 295 CCTAGG C^CTAGG
4766 CCTAGG
BbvCI 97 CCTCAGC CCTCAGC(-5/-2)
2705 GCTGAGG
BfrI 3865 CTTAAG C^TTAAG
5966 CTTAAG
BglII 304 AGATCT A^GATCT
3333 AGATCT
BlnI 295 CCTAGG C^CTAGG
4766 CCTAGG
BsaWI 4075 TCCGGA [AT]^CCGG[AT]
4311 TCCGGT
BsmBI 226 GAGACG CGTCTC(1/5)
4399 GAGACG
BspHI 3493 TCATGA T^CATGA
5019 TCATGA
BstAPI 770 GCATTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1256 GCAAACAGTGC
DraIII 2049 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
6264 CACATTGTG
DrdI 1197 GACAGAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4198 GACGGAAGTGTC
HaeII 2592 AGCGCT [GA]GCGC^[CT]
3484 GGCGCT
RcaI 3493 TCATGA T^CATGA
5019 TCATGA
SpeI 4630 ACTAGT A^CTAGT
5773 ACTAGT
SphI 290 GCATGC GCATG^C
3823 GCATGC
StuI 610 AGGCCT AGG^CCT
2362 AGGCCT
XcmI 315 CCACCACAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3117 CCACCAAGTTCATGG
XmnI 370 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5693 GAAGCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcgI BciVI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRI EcoRII
EcoRV EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII