##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BclI BcnI BfrI BglI
BglII BlnI BsaAI BsaBI BsaI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspMI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstYI BstZ17I Bsu36I CfoI
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MroI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
Sse8387I SspI SwaI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 353 GTCGAC GT^[AC][GT]AC
AflIII 977 ACATGT A^C[GA][CT]GT
AgeI 925 ACCGGT A^CCGGT
ApaI 839 GGGCCC GGGCC^C
AspHI 386 GAGCAC G[AT]GC[AT]^C
AvaI 381 CTCGGG C^[CT]CG[GA]G
BalI 666 TGGCCA TGG^CCA
BanI 302 GGCACC G^G[CT][GA]CC
BanII 839 GGGCCC G[GA]GC[CT]^C
BbsI 56 GAAGAC GAAGAC(2/6)
BcgI 303 GCACCGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 489 GGATAC GTATCC(6/5)
BfaI 132 CTAG C^TAG
BlpI 13 GCTGAGC GC^T[ACGT]AGC
BpmI 209 CTGGAG CTGGAG(16/14)
Bpu1102I 13 GCTGAGC GC^T[ACGT]AGC
BpuAI 56 GAAGAC GAAGAC(2/6)
BsaWI 925 ACCGGT [AT]^CCGG[AT]
BsgI 818 CTGCAC GTGCAG(16/14)
BsiHKAI 386 GAGCAC G[AT]GC[AT]^C
BsoBI 381 CTCGGG C^[CT]CG[GA]G
BspLU11I 977 ACATGT A^CATGT
BsrDI 457 CATTGC GCAATG(2/0)
BsrFI 925 ACCGGT [GA]^CCGG[CT]
BstEII 685 GGTGACC G^GT[ACGT]ACC
BstPI 685 GGTGACC G^GT[ACGT]ACC
BstXI 669 CCAGCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 13 GCTGAGC GC^T[ACGT]AGC
Cfr10I 925 ACCGGT [GA]^CCGG[CT]
DpnII 310 GATC ^GATC
DraI 920 TTTAAA TTT^AAA
DraII 838 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 601 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 76 CCGTGG C^C[GA][CT]GG
EcoO109I 838 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 685 GGTGACC G^GT[ACGT]ACC
HapII 926 CCGG C^CGG
HinfI 861 GATTC G^A[ACGT]TC
HpaII 926 CCGG C^CGG
MaeI 132 CTAG C^TAG
MaeII 707 ACGT A^CGT
MboI 310 GATC ^GATC
MluNI 666 TGGCCA TGG^CCA
MscI 666 TGGCCA TGG^CCA
MslI 169 CATCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 926 CCGG C^CGG
NdeII 310 GATC ^GATC
PinAI 925 ACCGGT A^CCGGT
SalI 353 GTCGAC G^TCGAC
Sau3AI 310 GATC ^GATC
SpeI 131 ACTAGT A^CTAGT
SphI 796 GCATGC GCATG^C
StuI 654 AGGCCT AGG^CCT
TfiI 861 GATTC G^A[AT]TC
XcmI 144 CCAGGGTCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 141 CCGC CCGC(-3/-1)
23 GCGG
AvaII 138 GGTCC G^G[AT]CC
739 GGACC
BmyI 386 GAGCAC G[AGT]GC[ACT]^C
839 GGGCCC
BseRI 285 GAGGAG GAGGAG(10/8)
650 GAGGAG
BsmAI 767 GAGAC GTCTC(1/5)
847 GAGAC
BsmFI 197 GGGAC GGGAC(10/14)
587 GTCCC
Bsp1286I 386 GAGCAC G[AGT]GC[ACT]^C
839 GGGCCC
Bsp1407I 33 TGTACA T^GTACA
974 TGTACA
BsrGI 33 TGTACA T^GTACA
974 TGTACA
BsrI 770 ACTGG ACTGG(1/-1)
258 CCAGT
DdeI 14 CTGAG C^T[ACGT]AG
572 CTAAG
EaeI 73 TGGCCG [CT]^GGCC[GA]
666 TGGCCA
EcoT14I 298 CCTTGG C^C[AT][AT]GG
494 CCAAGG
HincII 245 GTTGAC GT[CT]^[GA]AC
353 GTCGAC
HindII 245 GTTGAC GT[CT]^[GA]AC
353 GTCGAC
HphI 290 GGTGA GGTGA(8/7)
685 GGTGA
MwoI 461 GCTGTAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
609 GCAAGATTGGC
SspBI 33 TGTACA T^GTACA
974 TGTACA
StyI 298 CCTTGG C^C[AT][AT]GG
494 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AcyI AfaI AluI ApoI
BbvI BsaHI BsaJI BsiYI BslI
BssKI BstF5I BstNI Cac8I Cfr13I
EarI EcoRII Fnu4HI FokI HaeIII
HgaI Hin1I ItaI Ksp632I MaeIII
MboII MnlI MseI MvaI NlaIII
NlaIV NspI RsaI Sau96I ScrFI
SfaNI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I