##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AgeI        AhdI        AocI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BalI        
BamHI       BanII       BbrPI       BcgI        BclI        
BglI        BglII       BlnI        BsaAI       BsaBI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmI        BspDI       BspEI       BspHI       
BsrBI       BsrFI       BssHII      BssSI       BstBI       
BstEII      BstPI       BstUI       BstYI       Bsu36I      
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoT14I     
FbaI        FseI        FspI        HindIII     KpnI        
Ksp632I     KspI        MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MunI        MvnI        NaeI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SfaNI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    StuI        StyI        
SwaI        XbaI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AflII         636  CTTAAG               C^TTAAG
AflIII        228  ACATGT               A^C[GA][CT]GT
Alw44I        766  GTGCAC               G^TGCAC
AlwNI         260  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         766  GTGCAC               G^TGCAC
Asp700I       319  GAACTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         766  GTGCAC               G[AT]GC[AT]^C
AspI          735  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          837  CCCGAG               C^[CT]CG[GA]G
AvaII          61  GGTCC                G^G[AT]CC
BanI          857  GGCGCC               G^G[CT][GA]CC
BbeI          857  GGCGCC               GGCGC^C
BbvCI         828  CCTCAGC              CCTCAGC(-5/-2)
BbvI         1179  GCAGC                GCAGC(8/12)
BciVI          27  GGATAC               GTATCC(6/5)
BfaI          392  CTAG                 C^TAG
BfrI          636  CTTAAG               C^TTAAG
BlpI         1337  GCTGAGC              GC^T[ACGT]AGC
BpmI          761  CTGGAG               CTGGAG(16/14)
Bpu1102I     1337  GCTGAGC              GC^T[ACGT]AGC
BsiHKAI       766  GTGCAC               G[AT]GC[AT]^C
BsiYI          51  CCATGCATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           51  CCATGCATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         848  GGGAC                GGGAC(10/14)
BsoBI         837  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      420  TGTACA               T^GTACA
BspLU11I      228  ACATGT               A^CATGT
BspMI          34  GCAGGT               ACCTGC(4/8)
BsrGI         420  TGTACA               T^GTACA
Bst1107I     1322  GTATAC               GTA^TAC
BstAPI       1275  GCAGGGTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      1322  GTATAC               GTA^TAC
CelII        1337  GCTGAGC              GC^T[ACGT]AGC
DraI          606  TTTAAA               TTT^AAA
EcoRV        1254  GATATC               GAT^ATC
FauI          958  CCCGC                CCCGC(4/6)
Fnu4HI       1179  GCAGC                GC^[ACGT]GC
HaeII         857  GGCGCC               [GA]GCGC^[CT]
HgaI          869  GACGC                GACGC(5/10)
HincII       1021  GTTAAC               GT[CT]^[GA]AC
HindII       1021  GTTAAC               GT[CT]^[GA]AC
HpaI         1021  GTTAAC               GTT^AAC
ItaI         1179  GCAGC                GC^[ACGT]GC
KasI          857  GGCGCC               G^GCGCC
MaeI          392  CTAG                 C^TAG
MaeII         614  ACGT                 A^CGT
NarI          857  GGCGCC               GG^CGCC
NdeI           21  CATATG               CA^TATG
PflFI         735  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI          51  CCATGCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          626  CTGCAG               CTGCA^G
ScaI         1263  AGTACT               AGT^ACT
SexAI         913  ACCAGGT              A^CC[AT]GGT
SfoI          857  GGCGCC               GGC^GCC
SspBI         420  TGTACA               T^GTACA
SspI         1373  AATATT               AAT^ATT
TaqI          934  TCGA                 T^CGA
TseI         1179  GCAGC                G^C[AT]GC
Tth111I       735  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       934  TCGA                 T^CGA
Van91I         51  CCATGCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          450  CCAGACCAGTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          319  GAACTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI         1300  GTCTAC               GT^[AC][GT]AC
             1322  GTATAC              
AciI          955  CCGC                 CCGC(-3/-1)
              959  CCGC                
AcyI          857  GGCGCC               G[GA]^CG[CT]C
              869  GACGCC              
AfaI          421  GTAC                 GT^AC
             1264  GTAC                
AlwI          952  GATCC                GGATC(4/5)
              527  GGATC               
BbsI          546  GAAGAC               GAAGAC(2/6)
             1105  GTCTTC              
BmyI          766  GTGCAC               G[AGT]GC[ACT]^C
             1004  GTGCCC              
BpuAI         546  GAAGAC               GAAGAC(2/6)
             1105  GTCTTC              
BsaHI         857  GGCGCC               G[GA]^CG[CT]C
              869  GACGCC              
BsaI          631  GAGACC               GGTCTC(1/5)
              930  GGTCTC              
BsmBI         733  GAGACG               CGTCTC(1/5)
              900  GAGACG              
Bsp1286I      766  GTGCAC               G[AGT]GC[ACT]^C
             1004  GTGCCC              
BsrDI         531  CATTGC               GCAATG(2/0)
              383  GCAATG              
Cac8I         792  GCAAGC               GC[ACGT]^[ACGT]GC
              957  GCCCGC              
CfoI          858  GCGC                 GCG^C
             1395  GCGC                
Cfr13I         61  GGTCC                G^G[ACGT]CC
             1289  GGCCC               
EcoT22I        53  ATGCAT               ATGCA^T
             1079  ATGCAT              
HhaI          858  GCGC                 GCG^C
             1395  GCGC                
Hin1I         857  GGCGCC               G[GA]^CG[CT]C
              869  GACGCC              
HinP1I        858  GCGC                 G^CGC
             1395  GCGC                
HphI          752  TCACC                GGTGA(8/7)
              911  TCACC               
MwoI         1275  GCAGGGTTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1395  GCGCAAGGTGC         
NlaIV          60  GGGTCC               GG[ACGT]^[ACGT]CC
              857  GGCGCC              
NsiI           53  ATGCAT               ATGCA^T
             1079  ATGCAT              
NspI          228  ACATGT               [GA]CATG^[CT]
             1081  GCATGT              
PleI          250  GAGTC                GAGTC(4/5)
              739  GAGTC               
RsaI          421  GTAC                 GT^AC
             1264  GTAC                
Sau96I         61  GGTCC                G^G[ACGT]CC
             1289  GGCCC               
SmlI          443  CTCAAG               C^T[CT][GA]AG
              636  CTTAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AluI        BcnI        BsaJI       BsmAI       BsrI        
BssKI       BstF5I      BstNI       BstXI       DdeI        
DpnII       EcoRII      FokI        HaeIII      HapII       
HinfI       HpaII       MaeIII      MboI        MboII       
MnlI        MseI        MspI        MvaI        NciI        
NdeII       NlaIII      Sau3AI      ScrFI       SfcI        
TfiI        Tru9I       Tsp45I      Tsp509I     TspRI