##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        AspEI       AviII       AvrII       BalI        
BamHI       BanII       BbrPI       BcgI        BciVI       
BclI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DpnII       DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HgaI        HincII      HindII      
HindIII     HpaI        KspI        MaeII       MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MspA1I      MunI        NaeI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
SspBI       SspI        SwaI        XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I        168  GGTACC               G^GTACC
AcsI         1165  GAATTT               [GA]^AATT[CT]
AcyI            9  GGCGCC               G[GA]^CG[CT]C
AflII         283  CTTAAG               C^TTAAG
ApoI         1165  GAATTT               [GA]^AATT[CT]
Asp700I       299  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       168  GGTACC               G^GTACC
AspHI         546  GAGCAC               G[AT]GC[AT]^C
AspI          732  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          341  CTCGGG               C^[CT]CG[GA]G
BbeI            9  GGCGCC               GGCGC^C
BbsI          775  GAAGAC               GAAGAC(2/6)
BcnI          711  CCGGG                CC^[GC]GG
BfrI          283  CTTAAG               C^TTAAG
BglI          197  GCCAGGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         775  GAAGAC               GAAGAC(2/6)
BsaHI           9  GGCGCC               G[GA]^CG[CT]C
BsiHKAI       546  GAGCAC               G[AT]GC[AT]^C
BsmBI         568  CGTCTC               CGTCTC(1/5)
BsoBI         341  CTCGGG               C^[CT]CG[GA]G
BstAPI        365  GCAGGAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         581  CCATATTCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI          808  TTTAAA               TTT^AAA
DsaI          764  CCACGG               C^C[GA][CT]GG
EarI          571  CTCTTC               CTCTTC(1/4)
Hin1I           9  GGCGCC               G[GA]^CG[CT]C
KasI            9  GGCGCC               G^GCGCC
KpnI          168  GGTACC               GGTAC^C
Ksp632I       571  CTCTTC               CTCTTC(1/4)
NarI            9  GGCGCC               GG^CGCC
NciI          711  CCGGG                CC^[GC]GG
NspI          991  GCATGT               [GA]CATG^[CT]
PflFI         732  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         764  CCACGGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfcI          525  CTACAG               C^T[GA][CT]AG
SfoI            9  GGCGCC               GGC^GCC
SpeI          438  ACTAGT               A^CTAGT
StuI          143  AGGCCT               AGG^CCT
Tth111I       732  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        764  CCACGGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          299  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII         446  CGCG                 CG^CG
              820  CGCG                
AfaI          169  GTAC                 GT^AC
              442  GTAC                
AlwNI          41  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              538  CAGAGCCTG           
AvaII         534  GGACC                G^G[AT]CC
             1110  GGTCC               
BbvCI          32  CCTCAGC              CCTCAGC(-5/-2)
              405  CCTCAGC             
BfaI          439  CTAG                 C^TAG
              910  CTAG                
BpmI          394  CTGGAG               CTGGAG(16/14)
              415  CTGGAG              
BseRI         617  CTCCTC               GAGGAG(10/8)
              319  GAGGAG              
BsrDI        1018  CATTGC               GCAATG(2/0)
              470  GCAATG              
BstF5I        756  CATCC                GGATG(2/0)
             1189  GGATG               
BstUI         446  CGCG                 CG^CG
              820  CGCG                
EcoT14I       462  CCTTGG               C^C[AT][AT]GG
              485  CCAAGG              
FokI          756  CATCC                GGATG(9/13)
             1189  GGATG               
HaeII           9  GGCGCC               [GA]GCGC^[CT]
              882  GGCGCT              
HapII          13  CCGG                 C^CGG
              711  CCGG                
HpaII          13  CCGG                 C^CGG
              711  CCGG                
MaeI          439  CTAG                 C^TAG
              910  CTAG                
MslI          351  CACTGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              582  CATATTCATG          
MspI           13  CCGG                 C^CGG
              711  CCGG                
MvnI          446  CGCG                 CG^CG
              820  CGCG                
RsaI          169  GTAC                 GT^AC
              442  GTAC                
SfaNI         324  GATGC                GCATC(5/9)
              595  GATGC               
SmlI          103  CTTGAG               C^T[CT][GA]AG
              283  CTTAAG              
StyI          462  CCTTGG               C^C[AT][AT]GG
              485  CCAAGG              
Tsp509I       864  AATT                 ^AATT
             1166  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AluI        BanI        BbvI        BmyI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstNI       Cac8I       
CfoI        Cfr13I      DdeI        EcoRII      Fnu4HI      
HaeIII      HhaI        HinP1I      HinfI       HphI        
ItaI        MaeIII      MboII       MnlI        MseI        
MvaI        MwoI        NlaIII      NlaIV       Sau96I      
ScrFI       TaqI        TfiI        Tru9I       TseI        
Tsp45I      TspRI       TthHB8I