##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccII AccIII AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AviII AvrII BamHI BbrPI BciVI
BfrI BlnI BsaAI BsaBI BseAI
BsiWI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstUI BstZ17I
Cfr10I CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoT22I FseI FspI
KspI MamI MluI MroI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PinAI PmaCI
PmeI PmlI PshBI RsrII SacII
SalI SbfI SexAI SgrAI SnaBI
SphI Sse8387I SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 3454 GACGTC GACGT^C
Acc65I 3010 GGTACC G^GTACC
AflIII 2295 ACACGT A^C[GA][CT]GT
Asp718I 3010 GGTACC G^GTACC
AspI 4276 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 4 GGCGCC GGCGC^C
BcgI 635 GCACAAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3151 TGATCA T^GATCA
BglI 4022 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 1232 GAGACG CGTCTC(1/5)
Bsp1407I 2123 TGTACA T^GTACA
BspDI 734 ATCGAT AT^CGAT
BspHI 2827 TCATGA T^CATGA
BsrGI 2123 TGTACA T^GTACA
BstBI 770 TTCGAA TT^CGAA
ClaI 734 ATCGAT AT^CGAT
DraIII 4521 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4764 GACTTCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3826 CCATGG C^C[GA][CT]GG
EcoRI 3264 GAATTC G^AATTC
FauI 1270 GCGGG CCCGC(4/6)
FbaI 3151 TGATCA T^GATCA
HincII 2670 GTTAAC GT[CT]^[GA]AC
HindII 2670 GTTAAC GT[CT]^[GA]AC
HindIII 3904 AAGCTT A^AGCTT
HpaI 2670 GTTAAC GTT^AAC
KasI 4 GGCGCC G^GCGCC
KpnI 3010 GGTACC GGTAC^C
MfeI 1903 CAATTG C^AATTG
MslI 2658 CACCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1903 CAATTG C^AATTG
NarI 4 GGCGCC GG^CGCC
NcoI 3826 CCATGG C^CATGG
NspV 770 TTCGAA TT^CGAA
PflFI 4276 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4385 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 1275 CGATCG CGAT^CG
RcaI 2827 TCATGA T^CATGA
SacI 2331 GAGCTC GAGCT^C
SfiI 4021 GGCCACAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4 GGCGCC GGC^GCC
SfuI 770 TTCGAA TT^CGAA
SpeI 2564 ACTAGT A^CTAGT
SspBI 2123 TGTACA T^GTACA
Tth111I 4276 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4385 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3124 CCAATCTGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 3253 GTCTAC GT^[AC][GT]AC
5488 GTAGAC
AclI 2186 AACGTT AA^CGTT
3043 AACGTT
Alw44I 2796 GTGCAC G^TGCAC
3335 GTGCAC
AocI 14 CCTGAGG CC^T[ACGT]AGG
3523 CCTCAGG
ApaI 4029 GGGCCC GGGCC^C
4664 GGGCCC
ApaLI 2796 GTGCAC G^TGCAC
3335 GTGCAC
Asp700I 2250 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5091 GAACAGTTTC
BglII 1209 AGATCT A^GATCT
2345 AGATCT
BlpI 3138 GCTCAGC GC^T[ACGT]AGC
5810 GCTGAGC
Bpu1102I 3138 GCTCAGC GC^T[ACGT]AGC
5810 GCTGAGC
BsaWI 745 ACCGGA [AT]^CCGG[AT]
898 ACCGGA
BsmI 310 GAATGC GAATGC(1/-1)
5470 GCATTC
BsrDI 5058 CATTGC GCAATG(2/0)
5803 CATTGC
BstAPI 1530 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3855 GCACCCTCTGC
BstEII 476 GGTGACC G^GT[ACGT]ACC
1180 GGTCACC
BstPI 476 GGTGACC G^GT[ACGT]ACC
1180 GGTCACC
Bsu36I 14 CCTGAGG CC^T[ACGT]AGG
3523 CCTCAGG
CelII 3138 GCTCAGC GC^T[ACGT]AGC
5810 GCTGAGC
Eco81I 14 CCTGAGG CC^T[ACGT]AGG
3523 CCTCAGG
EcoO65I 476 GGTGACC G^GT[ACGT]ACC
1180 GGTCACC
EcoRV 534 GATATC GAT^ATC
749 GATATC
PaeR7I 1076 CTCGAG C^TCGAG
1118 CTCGAG
Psp1406I 2186 AACGTT AA^CGTT
3043 AACGTT
SapI 3483 GAAGAGC GCTCTTC(1/4)
1986 GCTCTTC
XhoI 1076 CTCGAG C^TCGAG
1118 CTCGAG
XmnI 2250 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5091 GAACAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI