##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AflII AflIII AgeI AscI
AseI AsnI Asp700I Asp718I BamHI
BbrPI BcgI BfrI BsaAI BsaBI
BsiEI BsiWI BspDI BspHI BspLU11I
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI EagI EclXI Eco52I EcoRI
EcoRV EcoT22I FseI HpaI KpnI
MamI MluI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI PvuI
RcaI RsrII SacI SalI SapI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SspI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 4901 GACGTC GACGT^C
AccIII 2123 TCCGGA T^CCGGA
AclI 3054 AACGTT AA^CGTT
AhdI 4850 GACGTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4526 GTGCAC G^TGCAC
Aor51HI 670 AGCGCT AGC^GCT
ApaLI 4526 GTGCAC G^TGCAC
AspEI 4850 GACGTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3389 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2440 TGCGCA TGC^GCA
AvrII 4116 CCTAGG C^CTAGG
BbeI 1060 GGCGCC GGCGC^C
BciVI 1831 GTATCC GTATCC(6/5)
BclI 487 TGATCA T^GATCA
BglII 5086 AGATCT A^GATCT
BlnI 4116 CCTAGG C^CTAGG
BseAI 2123 TCCGGA T^CCGGA
BsmI 417 GAATGC GAATGC(1/-1)
Bsp1407I 4630 TGTACA T^GTACA
BspEI 2123 TCCGGA T^CCGGA
BsrGI 4630 TGTACA T^GTACA
BssSI 1570 CTCGTG CACGAG(-5/-1)
BstEII 3691 GGTGACC G^GT[ACGT]ACC
BstPI 3691 GGTGACC G^GT[ACGT]ACC
Eam1105I 4850 GACGTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 670 AGCGCT AGC^GCT
EcoO65I 3691 GGTGACC G^GT[ACGT]ACC
FbaI 487 TGATCA T^GATCA
FspI 2440 TGCGCA TGC^GCA
HincII 42 GTTGAC GT[CT]^[GA]AC
HindII 42 GTTGAC GT[CT]^[GA]AC
KasI 1060 GGCGCC G^GCGCC
KspI 1409 CCGCGG CCGC^GG
MfeI 2841 CAATTG C^AATTG
MroI 2123 TCCGGA T^CCGGA
MunI 2841 CAATTG C^AATTG
NarI 1060 GGCGCC GG^CGCC
NheI 3194 GCTAGC G^CTAGC
PflFI 3389 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 3054 AACGTT AA^CGTT
SacII 1409 CCGCGG CCGC^GG
SexAI 1710 ACCAGGT A^CC[AT]GGT
SfoI 1060 GGCGCC GGC^GCC
SphI 3606 GCATGC GCATG^C
SspBI 4630 TGTACA T^GTACA
SwaI 4646 ATTTAAAT ATTT^AAAT
Tth111I 3389 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1094 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 3307 GATCC GGATC(4/5)
2196 GGATC
BglI 1104 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1243 GCCCAGGGGGC
BlpI 811 GCTCAGC GC^T[ACGT]AGC
4332 GCTAAGC
Bpu1102I 811 GCTCAGC GC^T[ACGT]AGC
4332 GCTAAGC
BsaWI 2123 TCCGGA [AT]^CCGG[AT]
2344 TCCGGT
BsrDI 260 CATTGC GCAATG(2/0)
4971 GCAATG
BstAPI 3502 GCACCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4284 GCAAGCCGTGC
BstYI 2196 GGATCT [GA]^GATC[CT]
5086 AGATCT
CelII 811 GCTCAGC GC^T[ACGT]AGC
4332 GCTAAGC
DrdI 1035 GACAAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1305 GACCACCTCGTC
MflI 2196 GGATCT [GA]^GATC[CT]
5086 AGATCT
NcoI 2256 CCATGG C^CATGG
4145 CCATGG
PshAI 1167 GACTGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1268 GACCCCGGTC
SbfI 2487 CCTGCAGG CCTGCA^GG
4338 CCTGCAGG
Sse8387I 2487 CCTGCAGG CCTGCA^GG
4338 CCTGCAGG
XhoII 2196 GGATCT [GA]^GATC[CT]
5086 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XmaCI XmaI