##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaHI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspMI
BsrBI BsrGI BssHII BssSI BstBI
BstEII BstPI BstUI BstXI Bsu36I
CelII ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
FauI FbaI FseI FspI HgaI
Hin1I HindIII KasI KpnI KspI
MluI MluNI MroI MscI MspA1I
MvnI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SpeI Sse8387I
SspBI SwaI Tth111I Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 2504 GTATAC GT^[AC][GT]AC
AgeI 207 ACCGGT A^CCGGT
AvaII 1869 GGACC G^G[AT]CC
BanI 2719 GGTGCC G^G[CT][GA]CC
BciVI 1749 GTATCC GTATCC(6/5)
BcnI 1796 CCCGG CC^[GC]GG
BglII 386 AGATCT A^GATCT
BsaBI 1551 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 207 ACCGGT [AT]^CCGG[AT]
BsmFI 875 GTCCC GGGAC(10/14)
BsmI 313 GAATGC GAATGC(1/-1)
BspHI 1144 TCATGA T^CATGA
Bst1107I 2504 GTATAC GTA^TAC
BstYI 386 AGATCT [GA]^GATC[CT]
BstZ17I 2504 GTATAC GTA^TAC
CfoI 437 GCGC GCG^C
EcoRI 1839 GAATTC G^AATTC
EcoT14I 1986 CCATGG C^C[AT][AT]GG
EcoT22I 571 ATGCAT ATGCA^T
HaeII 436 GGCGCT [GA]GCGC^[CT]
HhaI 437 GCGC GCG^C
HinP1I 437 GCGC G^CGC
HpaI 1885 GTTAAC GTT^AAC
MamI 1551 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 386 AGATCT [GA]^GATC[CT]
NciI 1796 CCCGG CC^[GC]GG
NcoI 1986 CCATGG C^CATGG
NdeI 1825 CATATG CA^TATG
NheI 527 GCTAGC G^CTAGC
NsiI 571 ATGCAT ATGCA^T
PinAI 207 ACCGGT A^CCGGT
RcaI 1144 TCATGA T^CATGA
SnaBI 2917 TACGTA TAC^GTA
SphI 1791 GCATGC GCATG^C
SspI 772 AATATT AAT^ATT
StuI 1628 AGGCCT AGG^CCT
StyI 1986 CCATGG C^C[AT][AT]GG
Tsp45I 1333 GTGAC ^GT[GC]AC
XhoII 386 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AciI 12 CCGC CCGC(-3/-1)
15 CCGC
AflIII 1232 ACATGT A^C[GA][CT]GT
2775 ACATGT
AlwI 885 GATCC GGATC(4/5)
2514 GATCC
AlwNI 682 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2688 CAGAGACTG
BbsI 2618 GAAGAC GAAGAC(2/6)
694 GTCTTC
BpuAI 2618 GAAGAC GAAGAC(2/6)
694 GTCTTC
BsaAI 1864 TACGTG [CT]AC^GT[GA]
2917 TACGTA
BsaI 393 GAGACC GGTCTC(1/5)
1959 GGTCTC
BsiYI 1193 CCTGAGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2710 CCTTTTGAAGG
BslI 1193 CCTGAGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2710 CCTTTTGAAGG
BspLU11I 1232 ACATGT A^CATGT
2775 ACATGT
BsrFI 23 GCCGGT [GA]^CCGG[CT]
207 ACCGGT
Cfr10I 23 GCCGGT [GA]^CCGG[CT]
207 ACCGGT
Cfr13I 320 GGCCC G^G[ACGT]CC
1869 GGACC
DsaI 878 CCGTGG C^C[GA][CT]GG
1986 CCATGG
EarI 53 CTCTTC CTCTTC(1/4)
1247 GAAGAG
EcoNI 1193 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2710 CCTTTTGAAGG
EcoRV 672 GATATC GAT^ATC
1936 GATATC
HincII 536 GTTGAC GT[CT]^[GA]AC
1885 GTTAAC
HindII 536 GTTGAC GT[CT]^[GA]AC
1885 GTTAAC
Ksp632I 53 CTCTTC CTCTTC(1/4)
1247 GAAGAG
MaeII 1865 ACGT A^CGT
2918 ACGT
MfeI 1950 CAATTG C^AATTG
2509 CAATTG
MslI 296 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2724 CATTCTTATG
MunI 1950 CAATTG C^AATTG
2509 CAATTG
SacI 835 GAGCTC GAGCT^C
846 GAGCTC
Sau96I 320 GGCCC G^G[ACGT]CC
1869 GGACC
SfcI 1537 CTACAG C^T[GA][CT]AG
1546 CTATAG
TaqI 272 TCGA T^CGA
1837 TCGA
TspRI 334 CACTG CA[GC]TG[ACGT][ACGT]^
986 CACTG
TthHB8I 272 TCGA T^CGA
1837 TCGA
XbaI 389 TCTAGA T^CTAGA
2308 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
AspHI BanII BbvI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsmAI
Bsp1286I BsrDI BsrI BssKI BstAPI
BstF5I BstNI Cac8I DdeI DpnII
DraI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI ScrFI SfaNI SmlI TfiI
Tru9I TseI Tsp509I XcmI XmnI