##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI PvuI RsrII SacII
SalI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SspBI StuI
SwaI Tth111I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1202 GTAGAC GT^[AC][GT]AC
AciI 1375 CCGC CCGC(-3/-1)
AclI 1457 AACGTT AA^CGTT
AcyI 1136 GGCGCC G[GA]^CG[CT]C
AflIII 1313 ACATGT A^C[GA][CT]GT
AlwNI 1572 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1622 GGGCCC GGGCC^C
AspHI 354 GAGCTC G[AT]GC[AT]^C
AvaII 170 GGACC G^G[AT]CC
BanI 1136 GGCGCC G^G[CT][GA]CC
BbeI 1136 GGCGCC GGCGC^C
BbsI 993 GAAGAC GAAGAC(2/6)
BbvCI 1352 GCTGAGG CCTCAGC(-5/-2)
BpuAI 993 GAAGAC GAAGAC(2/6)
BsaHI 1136 GGCGCC G[GA]^CG[CT]C
BsiHKAI 354 GAGCTC G[AT]GC[AT]^C
BspHI 445 TCATGA T^CATGA
BspLU11I 1313 ACATGT A^CATGT
BstYI 985 GGATCT [GA]^GATC[CT]
DsaI 27 CCATGG C^C[GA][CT]GG
FauI 1374 CCCGC CCCGC(4/6)
Hin1I 1136 GGCGCC G[GA]^CG[CT]C
KasI 1136 GGCGCC G^GCGCC
MflI 985 GGATCT [GA]^GATC[CT]
MspA1I 1614 CAGCTG C[AC]G^C[GT]G
NarI 1136 GGCGCC GG^CGCC
NcoI 27 CCATGG C^CATGG
PflMI 1613 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1457 AACGTT AA^CGTT
PvuII 1614 CAGCTG CAG^CTG
RcaI 445 TCATGA T^CATGA
SacI 354 GAGCTC GAGCT^C
SbfI 15 CCTGCAGG CCTGCA^GG
SfoI 1136 GGCGCC GGC^GCC
SphI 584 GCATGC GCATG^C
Sse8387I 15 CCTGCAGG CCTGCA^GG
SspI 1539 AATATT AAT^ATT
Van91I 1613 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1519 TCTAGA T^CTAGA
XhoII 985 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 1 CGCG CG^CG
1376 CGCG
AcsI 645 AAATTC [GA]^AATT[CT]
826 GAATTT
AlwI 985 GGATC GGATC(4/5)
1591 GGATC
ApoI 645 AAATTC [GA]^AATT[CT]
826 GAATTT
BalI 24 TGGCCA TGG^CCA
564 TGGCCA
BcnI 53 CCGGG CC^[GC]GG
1256 CCCGG
BpmI 351 CTGGAG CTGGAG(16/14)
1395 CTGGAG
BsmAI 1310 GAGAC GTCTC(1/5)
506 GTCTC
BsmFI 1060 GGGAC GGGAC(10/14)
1484 GTCCC
BstUI 1 CGCG CG^CG
1376 CGCG
EcoT14I 27 CCATGG C^C[AT][AT]GG
45 CCAAGG
HaeII 31 GGCGCT [GA]GCGC^[CT]
1136 GGCGCC
HindIII 129 AAGCTT A^AGCTT
569 AAGCTT
MluNI 24 TGGCCA TGG^CCA
564 TGGCCA
MscI 24 TGGCCA TGG^CCA
564 TGGCCA
MslI 614 CATTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
958 CATGTTAGTG
MvnI 1 CGCG CG^CG
1376 CGCG
NciI 53 CCGGG CC^[GC]GG
1256 CCCGG
SapI 929 GCTCTTC GCTCTTC(1/4)
1022 GCTCTTC
ScaI 1153 AGTACT AGT^ACT
1719 AGTACT
SmlI 71 CTCAAG C^T[CT][GA]AG
1723 CTCAAG
StyI 27 CCATGG C^C[AT][AT]GG
45 CCAAGG
TaqI 490 TCGA T^CGA
1159 TCGA
TthHB8I 490 TCGA T^CGA
1159 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AfaI AluI Asp700I BanII BbvI
BfaI BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NdeI
NdeII NlaIII NlaIV NspI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XmnI