##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AocI AscI Asp718I AspEI
AviII AvrII BalI BamHI BglII
BlnI BsaBI BseAI BspDI BspEI
BspHI BspLU11I BsrDI BstBI Bsu36I
ClaI CpoI DrdI Eam1105I Eco81I
EcoRI EcoRV FseI FspI HincII
HindII HpaI KpnI MamI MfeI
MluNI MroI MscI MunI NcoI
NdeI NheI NotI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AflII 3465 CTTAAG C^TTAAG
Alw44I 3193 GTGCAC G^TGCAC
AlwI 1036 GGATC GGATC(4/5)
ApaI 1194 GGGCCC GGGCC^C
ApaLI 3193 GTGCAC G^TGCAC
Asp700I 3312 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 350 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 2156 GTCTTC GAAGAC(2/6)
BciVI 1180 GTATCC GTATCC(6/5)
BclI 627 TGATCA T^GATCA
BfrI 3465 CTTAAG C^TTAAG
BglI 477 GCCTGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1411 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1411 GCTCAGC GC^T[ACGT]AGC
BpuAI 2156 GTCTTC GAAGAC(2/6)
BsaWI 720 ACCGGA [AT]^CCGG[AT]
BsiWI 2261 CGTACG C^GTACG
BssHII 454 GCGCGC G^CGCGC
BssSI 3035 CACGAG CACGAG(-5/-1)
BstEII 2511 GGTCACC G^GT[ACGT]ACC
BstPI 2511 GGTCACC G^GT[ACGT]ACC
BstYI 1036 GGATCT [GA]^GATC[CT]
CelII 1411 GCTCAGC GC^T[ACGT]AGC
DraI 1810 TTTAAA TTT^AAA
EcoO65I 2511 GGTCACC G^GT[ACGT]ACC
EcoT22I 1820 ATGCAT ATGCA^T
FbaI 627 TGATCA T^GATCA
HindIII 2728 AAGCTT A^AGCTT
KspI 56 CCGCGG CCGC^GG
MflI 1036 GGATCT [GA]^GATC[CT]
MluI 1221 ACGCGT A^CGCGT
NruI 2036 TCGCGA TCG^CGA
NsiI 1820 ATGCAT ATGCA^T
PflFI 350 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 56 CCGCGG CCGC^GG
ScaI 1070 AGTACT AGT^ACT
SexAI 2493 ACCAGGT A^CC[AT]GGT
SphI 181 GCATGC GCATG^C
StuI 2650 AGGCCT AGG^CCT
TaqI 450 TCGA T^CGA
TfiI 1797 GATTC G^A[AT]TC
Tth111I 350 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 450 TCGA T^CGA
XbaI 1694 TCTAGA T^CTAGA
XhoII 1036 GGATCT [GA]^GATC[CT]
XmnI 3312 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcsI 2583 GAATTT [GA]^AATT[CT]
2840 AAATTT
Aor51HI 734 AGCGCT AGC^GCT
1323 AGCGCT
ApoI 2583 GAATTT [GA]^AATT[CT]
2840 AAATTT
AspHI 3193 GTGCAC G[AT]GC[AT]^C
3483 GTGCTC
BbeI 78 GGCGCC GGCGC^C
647 GGCGCC
BbrPI 877 CACGTG CAC^GTG
1031 CACGTG
BbvCI 256 CCTCAGC CCTCAGC(-5/-2)
1784 GCTGAGG
BcgI 850 CGACCACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
441 GCAGCCCCATCG
BsaAI 877 CACGTG [CT]AC^GT[GA]
1031 CACGTG
BsiEI 59 CGGCCG CG[GA][CT]^CG
3179 CGGCCG
BsiHKAI 3193 GTGCAC G[AT]GC[AT]^C
3483 GTGCTC
BsmBI 355 CGTCTC CGTCTC(1/5)
140 GAGACG
Bsp1407I 495 TGTACA T^GTACA
2831 TGTACA
BspMI 2520 ACCTGC ACCTGC(4/8)
1288 GCAGGT
BsrBI 431 GAGCGG CCGCTC(-3/-3)
602 GAGCGG
BsrGI 495 TGTACA T^GTACA
2831 TGTACA
Bst1107I 1457 GTATAC GTA^TAC
2281 GTATAC
BstZ17I 1457 GTATAC GTA^TAC
2281 GTATAC
EagI 59 CGGCCG C^GGCCG
3179 CGGCCG
EarI 3357 CTCTTC CTCTTC(1/4)
1465 GAAGAG
EclXI 59 CGGCCG C^GGCCG
3179 CGGCCG
Eco47III 734 AGCGCT AGC^GCT
1323 AGCGCT
Eco52I 59 CGGCCG C^GGCCG
3179 CGGCCG
EcoNI 933 CCTCTCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3116 CCTGGGACAGG
KasI 78 GGCGCC G^GCGCC
647 GGCGCC
Ksp632I 3357 CTCTTC CTCTTC(1/4)
1465 GAAGAG
NaeI 572 GCCGGC GCC^GGC
811 GCCGGC
NarI 78 GGCGCC GG^CGCC
647 GGCGCC
NgoMIV 572 GCCGGC G^CCGGC
811 GCCGGC
PflMI 2018 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2726 CCAAGCTTTGG
PmaCI 877 CACGTG CAC^GTG
1031 CACGTG
PmlI 877 CACGTG CAC^GTG
1031 CACGTG
PpuMI 330 AGGACCT [GA]G^G[AT]CC[CT]
3184 GGGTCCC
SfoI 78 GGCGCC GGC^GCC
647 GGCGCC
SmlI 1390 CTTGAG C^T[CT][GA]AG
3465 CTTAAG
SspBI 495 TGTACA T^GTACA
2831 TGTACA
Van91I 2018 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2726 CCAAGCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwNI AseI AsnI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XmaCI XmaI