##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AflII       
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BbeI        BciVI       BclI        
BfrI        BglII       BlnI        BsaBI       BsaHI       
BseAI       BsiEI       BsiWI       Bsp1407I    BspDI       
BspEI       BspHI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstZ17I     ClaI        
CpoI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRV       FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NotI        NruI        PacI        PflFI       PmeI        
PshBI       PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SfiI        SfoI        
SgrAI       SnaBI       SphI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI         2230  GTCTAC               GT^[AC][GT]AC
AccII          47  CGCG                 CG^CG
AclI         1472  AACGTT               AA^CGTT
AflIII        765  ACATGT               A^C[GA][CT]GT
AgeI         1017  ACCGGT               A^CCGGT
ApaI          921  GGGCCC               GGGCC^C
Asp700I      2869  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         3091  TGGCCA               TGG^CCA
BamHI         114  GGATCC               G^GATCC
BbrPI        3087  CACGTG               CAC^GTG
BbsI         2869  GAAGAC               GAAGAC(2/6)
BbvCI        1546  CCTCAGC              CCTCAGC(-5/-2)
BcgI         2847  GCAGACTTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         1746  GCCAGGTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        2869  GAAGAC               GAAGAC(2/6)
BsaAI        3087  CACGTG               [CT]AC^GT[GA]
BsaWI        1017  ACCGGT               [AT]^CCGG[AT]
BsmBI        1928  CGTCTC               CGTCTC(1/5)
BspLU11I      765  ACATGT               A^CATGT
BsrBI        1769  CCGCTC               CCGCTC(-3/-3)
BsrFI        1017  ACCGGT               [GA]^CCGG[CT]
BstEII       3115  GGTGACC              G^GT[ACGT]ACC
BstPI        3115  GGTGACC              G^GT[ACGT]ACC
BstUI          47  CGCG                 CG^CG
BstYI         114  GGATCC               [GA]^GATC[CT]
Cfr10I       1017  ACCGGT               [GA]^CCGG[CT]
DraI         2802  TTTAAA               TTT^AAA
DraIII       2483  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         3091  TGGCCA               [CT]^GGCC[GA]
EcoNI        2128  CCTTTGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      3115  GGTGACC              G^GT[ACGT]ACC
EcoT22I       711  ATGCAT               ATGCA^T
HpaI         1469  GTTAAC               GTT^AAC
MflI          114  GGATCC               [GA]^GATC[CT]
MluNI        3091  TGGCCA               TGG^CCA
MscI         3091  TGGCCA               TGG^CCA
MvnI           47  CGCG                 CG^CG
NheI         2642  GCTAGC               G^CTAGC
NsiI          711  ATGCAT               ATGCA^T
PinAI        1017  ACCGGT               A^CCGGT
PmaCI        3087  CACGTG               CAC^GTG
PmlI         3087  CACGTG               CAC^GTG
PshAI        2238  GACAGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1472  AACGTT               AA^CGTT
PstI         3080  CTGCAG               CTGCA^G
SapI         1455  GCTCTTC              GCTCTTC(1/4)
SexAI        2930  ACCTGGT              A^CC[AT]GGT
SpeI         1854  ACTAGT               A^CTAGT
XhoII         114  GGATCC               [GA]^GATC[CT]
XmnI         2869  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AlwI          115  GATCC                GGATC(4/5)
              114  GGATC               
AocI          618  CCTCAGG              CC^T[ACGT]AGG
             1224  CCTCAGG             
BsmI         1987  GAATGC               GAATGC(1/-1)
             2808  GAATGC              
BspMI        1809  GCAGGT               ACCTGC(4/8)
             3039  GCAGGT              
BstBI         264  TTCGAA               TT^CGAA
              744  TTCGAA              
BstXI         637  CCACATGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1356  CCATCGGACTGG        
Bsu36I        618  CCTCAGG              CC^T[ACGT]AGG
             1224  CCTCAGG             
CfoI           46  GCGC                 GCG^C
             2419  GCGC                
DrdI          760  GACCAACATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2754  GACGGGAAAGTC        
Eco81I        618  CCTCAGG              CC^T[ACGT]AGG
             1224  CCTCAGG             
EcoRI        1256  GAATTC               G^AATTC
             1587  GAATTC              
HhaI           46  GCGC                 GCG^C
             2419  GCGC                
HinP1I         46  GCGC                 G^CGC
             2419  GCGC                
HincII        139  GTCAAC               GT[CT]^[GA]AC
             1469  GTTAAC              
HindII        139  GTCAAC               GT[CT]^[GA]AC
             1469  GTTAAC              
NcoI          477  CCATGG               C^CATGG
             1615  CCATGG              
NspI          765  ACATGT               [GA]CATG^[CT]
             2601  ACATGC              
NspV          264  TTCGAA               TT^CGAA
              744  TTCGAA              
PaeR7I        105  CTCGAG               C^TCGAG
              876  CTCGAG              
SfuI          264  TTCGAA               TT^CGAA
              744  TTCGAA              
SmaI          231  CCCGGG               CCC^GGG
              360  CCCGGG              
XcmI          634  CCACCACATGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2108  CCAGCTCTCTTCTGG     
XhoI          105  CTCGAG               C^TCGAG
              876  CTCGAG              
XmaCI         231  CCCGGG               C^CCGGG
              360  CCCGGG              
XmaI          231  CCCGGG               C^CCGGG
              360  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwNI       
ApoI        AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BfaI        BlpI        
BmyI        BpmI        Bpu1102I    BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       CelII       
Cfr13I      DdeI        DpnII       DraII       DsaI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PpuMI       PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I