##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflIII      AgeI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspI        AvrII       BbeI        BbrPI       
BbsI        BcgI        BclI        BlnI        BpuAI       
BsaAI       BsaBI       BsaHI       BseAI       BsiEI       
BsiWI       Bsp1407I    BspEI       BspHI       BspLU11I    
BspMI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstEII      BstPI       BstZ17I     Bsu36I      
Cfr10I      CpoI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRV       
FbaI        FseI        HaeII       Hin1I       HpaI        
KasI        KpnI        KspI        MamI        MluI        
MroI        NaeI        NarI        NgoMIV      NotI        
NruI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfoI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI         2238  GTCTAC               GT^[AC][GT]AC
AccII        3215  CGCG                 CG^CG
AflII        1479  CTTAAG               C^TTAAG
AhdI            7  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI           7  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         3183  CCCGGG               C^[CT]CG[GA]G
AviII         860  TGCGCA               TGC^GCA
BbvCI        3026  GCTGAGG              CCTCAGC(-5/-2)
BciVI         891  GTATCC               GTATCC(6/5)
BfrI         1479  CTTAAG               C^TTAAG
BglI         1963  GCCACGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         2698  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2698  GCTCAGC              GC^T[ACGT]AGC
BsaI         3059  GAGACC               GGTCTC(1/5)
BsaWI        1999  ACCGGA               [AT]^CCGG[AT]
BsmBI        1607  GAGACG               CGTCTC(1/5)
BsoBI        3183  CCCGGG               C^[CT]CG[GA]G
BspDI         865  ATCGAT               AT^CGAT
BsrBI         537  CCGCTC               CCGCTC(-3/-3)
BsrDI          45  GCAATG               GCAATG(2/0)
BstBI         809  TTCGAA               TT^CGAA
BstUI        3215  CGCG                 CG^CG
CelII        2698  GCTCAGC              GC^T[ACGT]AGC
ClaI          865  ATCGAT               AT^CGAT
DraI          264  TTTAAA               TTT^AAA
DraII        2801  GGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI          720  GACATTTCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I        7  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     2801  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT22I      1728  ATGCAT               ATGCA^T
FspI          860  TGCGCA               TGC^GCA
HgaI          401  GACGC                GACGC(5/10)
HindIII       452  AAGCTT               A^AGCTT
MspA1I        116  CAGCTG               C[AC]G^C[GT]G
MvnI         3215  CGCG                 CG^CG
NdeI         2871  CATATG               CA^TATG
NheI         3071  GCTAGC               G^CTAGC
NsiI         1728  ATGCAT               ATGCA^T
NspV          809  TTCGAA               TT^CGAA
PpuMI        2801  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII         116  CAGCTG               CAG^CTG
SacI         2245  GAGCTC               GAGCT^C
SfcI          668  CTACAG               C^T[GA][CT]AG
SfiI         1962  GGCCACGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          809  TTCGAA               TT^CGAA
SmaI         3183  CCCGGG               CCC^GGG
SphI         3128  GCATGC               GCATG^C
XbaI         2787  TCTAGA               T^CTAGA
XmaCI        3183  CCCGGG               C^CCGGG
XmaI         3183  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwNI        1696  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1718  CAGTGGCTG           
AspHI        2245  GAGCTC               G[AT]GC[AT]^C
             2926  GTGCTC              
BalI         1739  TGGCCA               TGG^CCA
             1961  TGGCCA              
BamHI        2002  GGATCC               G^GATCC
             2449  GGATCC              
BanI         1215  GGCACC               G^G[CT][GA]CC
             1618  GGTGCC              
BpmI          838  CTCCAG               CTGGAG(16/14)
             1543  CTGGAG              
BsgI           23  GTGCAG               GTGCAG(16/14)
             1310  GTGCAG              
BsiHKAI      2245  GAGCTC               G[AT]GC[AT]^C
             2926  GTGCTC              
DraIII       1207  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2920  CACTCAGTG           
EaeI         1739  TGGCCA               [CT]^GGCC[GA]
             1961  TGGCCA              
FauI          705  GCGGG                CCCGC(4/6)
             3032  GCGGG               
HincII        427  GTCAAC               GT[CT]^[GA]AC
              879  GTCAAC              
HindII        427  GTCAAC               GT[CT]^[GA]AC
              879  GTCAAC              
MfeI          155  CAATTG               C^AATTG
             1562  CAATTG              
MluNI        1739  TGGCCA               TGG^CCA
             1961  TGGCCA              
MscI         1739  TGGCCA               TGG^CCA
             1961  TGGCCA              
MslI          547  CATGGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              957  CATCCTGGTG          
MunI          155  CAATTG               C^AATTG
             1562  CAATTG              
PflMI        1742  CCAACCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2356  CCAAGGTTTGG         
SapI         1524  GAAGAGC              GCTCTTC(1/4)
             2247  GCTCTTC             
StuI         1787  AGGCCT               AGG^CCT
             2552  AGGCCT              
Van91I       1742  CCAACCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2356  CCAAGGTTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AvaII       BanII       BbvI        
BcnI        BfaI        BglII       BmyI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BsrI        BssKI       BssSI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EarI        EcoRI       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
XmnI