##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvrII BalI BamHI BanI BbeI
BbvCI BcgI BclI BcnI BglI
BglII BlnI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI BspDI BspEI
BspHI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstZ17I
Cac8I Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRV FauI FbaI FseI
HaeII HapII Hin1I HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI PacI PaeR7I
PflFI PinAI PleI PpuMI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SphI Sse8387I StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AlwNI 2501 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2590 CCTGAGG CC^T[ACGT]AGG
AviII 1671 TGCGCA TGC^GCA
BanII 2346 GGGCTC G[GA]GC[CT]^C
BbrPI 1707 CACGTG CAC^GTG
BbsI 119 GAAGAC GAAGAC(2/6)
BbvI 3180 GCTGC GCAGC(8/12)
BciVI 2366 GTATCC GTATCC(6/5)
BlpI 2151 GCTTAGC GC^T[ACGT]AGC
BmyI 2346 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 2151 GCTTAGC GC^T[ACGT]AGC
BpuAI 119 GAAGAC GAAGAC(2/6)
BseRI 1907 CTCCTC GAGGAG(10/8)
BsmI 1833 GCATTC GAATGC(1/-1)
Bsp1286I 2346 GGGCTC G[AGT]GC[ACT]^C
BspMI 2509 GCAGGT ACCTGC(4/8)
BstBI 3061 TTCGAA TT^CGAA
BstXI 2557 CCAACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1222 AGATCC [GA]^GATC[CT]
Bsu36I 2590 CCTGAGG CC^T[ACGT]AGG
CelII 2151 GCTTAGC GC^T[ACGT]AGC
Eco81I 2590 CCTGAGG CC^T[ACGT]AGG
EcoT14I 2573 CCAAGG C^C[AT][AT]GG
EcoT22I 2385 ATGCAT ATGCA^T
Fnu4HI 3180 GCTGC GC^[ACGT]GC
FspI 1671 TGCGCA TGC^GCA
HgaI 3183 GCGTC GACGC(5/10)
HincII 3301 GTTGAC GT[CT]^[GA]AC
HindII 3301 GTTGAC GT[CT]^[GA]AC
ItaI 3180 GCTGC GC^[ACGT]GC
MflI 1222 AGATCC [GA]^GATC[CT]
NsiI 2385 ATGCAT ATGCA^T
NspV 3061 TTCGAA TT^CGAA
PflMI 2732 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1707 CACGTG CAC^GTG
PmeI 1311 GTTTAAAC GTTT^AAAC
PmlI 1707 CACGTG CAC^GTG
PshAI 1883 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2507 CTGCAG CTGCA^G
SfuI 3061 TTCGAA TT^CGAA
SnaBI 74 TACGTA TAC^GTA
SspI 2886 AATATT AAT^ATT
StyI 2573 CCAAGG C^C[AT][AT]GG
TaqI 3062 TCGA T^CGA
TseI 3180 GCTGC G^C[AT]GC
Tsp45I 1881 GTGAC ^GT[GC]AC
TspRI 934 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 3062 TCGA T^CGA
Van91I 2732 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1222 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 2004 GTAGAC GT^[AC][GT]AC
2226 GTCTAC
AccII 1892 CGCG CG^CG
2534 CGCG
AflII 2318 CTTAAG C^TTAAG
3450 CTTAAG
AlwI 1223 GATCC GGATC(4/5)
2584 GGATC
BfrI 2318 CTTAAG C^TTAAG
3450 CTTAAG
BsaAI 74 TACGTA [CT]AC^GT[GA]
1707 CACGTG
BsgI 243 CTGCAC GTGCAG(16/14)
2868 GTGCAG
BsrDI 2768 CATTGC GCAATG(2/0)
457 GCAATG
BstUI 1892 CGCG CG^CG
2534 CGCG
CfoI 1672 GCGC GCG^C
2533 GCGC
EcoNI 1142 CCTTTAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3320 CCTGAAGTAGG
HhaI 1672 GCGC GCG^C
2533 GCGC
HinP1I 1672 GCGC G^CGC
2533 GCGC
HindIII 487 AAGCTT A^AGCTT
1410 AAGCTT
MvnI 1892 CGCG CG^CG
2534 CGCG
SfaNI 3263 GATGC GCATC(5/9)
3316 GATGC
SfcI 1828 CTACAG C^T[GA][CT]AG
2507 CTGCAG
SpeI 560 ACTAGT A^CTAGT
1483 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApoI AvaII BfaI BpmI BsaJI
BsiYI BslI BsmAI Bsp1407I BspLU11I
BsrGI BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII DraI EarI
EcoRI EcoRII FokI HaeIII HinfI
HphI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MvaI NdeII NlaIII NspI RsaI
Sau3AI Sau96I ScrFI SmlI SspBI
TfiI Tru9I Tsp509I