##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AviII AvrII BbeI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsiWI BspDI BsrBI BssHII Bst1107I
BstBI BstZ17I CelII ClaI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoT22I FbaI FseI FspI HincII
HindII HpaI KasI KpnI KspI
MfeI MluI MunI NarI NcoI
NdeI NotI NruI NsiI NspV
PinAI PmeI PshAI SacII SalI
SapI SbfI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 1744 TCCGGA T^CCGGA
AclI 110 AACGTT AA^CGTT
AflIII 2169 ACATGT A^C[GA][CT]GT
AseI 3040 ATTAAT AT^TAAT
AsnI 3040 ATTAAT AT^TAAT
Asp700I 824 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2633 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1476 GGATCC G^GATCC
BbrPI 2417 CACGTG CAC^GTG
BciVI 1097 GTATCC GTATCC(6/5)
BsaBI 895 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1744 TCCGGA [AT]^CCGG[AT]
BseAI 1744 TCCGGA T^CCGGA
BsiEI 481 CGATCG CG[GA][CT]^CG
BsmBI 1574 GAGACG CGTCTC(1/5)
BspEI 1744 TCCGGA T^CCGGA
BspLU11I 2169 ACATGT A^CATGT
BsrDI 1236 GCAATG GCAATG(2/0)
BssSI 1343 CACGAG CACGAG(-5/-1)
BstEII 1750 GGTGACC G^GT[ACGT]ACC
BstPI 1750 GGTGACC G^GT[ACGT]ACC
CpoI 1784 CGGACCG CG^G[AT]CCG
EcoNI 3241 CCTTTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1750 GGTGACC G^GT[ACGT]ACC
EcoRV 805 GATATC GAT^ATC
HindIII 532 AAGCTT A^AGCTT
MamI 895 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1744 TCCGGA T^CCGGA
PacI 3037 TTAATTAA TTAAT^TAA
PaeR7I 2010 CTCGAG C^TCGAG
PflFI 2633 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2417 CACGTG CAC^GTG
PmlI 2417 CACGTG CAC^GTG
PshBI 3040 ATTAAT AT^TAAT
Psp1406I 110 AACGTT AA^CGTT
PvuI 481 CGATCG CGAT^CG
RsrII 1784 CGGACCG CG^G[AT]CCG
SacI 23 GAGCTC GAGCT^C
ScaI 1446 AGTACT AGT^ACT
SexAI 1068 ACCTGGT A^CC[AT]GGT
SfiI 2422 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 2633 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2010 CTCGAG C^TCGAG
XmnI 824 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1568 GTCTAC GT^[AC][GT]AC
1868 GTCTAC
AcyI 2457 GACGCC G[GA]^CG[CT]C
2897 GACGCC
AocI 2136 CCTTAGG CC^T[ACGT]AGG
3274 CCTTAGG
ApaI 862 GGGCCC GGGCC^C
2647 GGGCCC
BglI 2423 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2460 GCCAACTTGGC
BsaAI 1862 TACGTG [CT]AC^GT[GA]
2417 CACGTG
BsaHI 2457 GACGCC G[GA]^CG[CT]C
2897 GACGCC
BsaI 398 GAGACC GGTCTC(1/5)
519 GAGACC
Bsp1407I 631 TGTACA T^GTACA
1806 TGTACA
BspHI 700 TCATGA T^CATGA
1830 TCATGA
BsrGI 631 TGTACA T^GTACA
1806 TGTACA
BstAPI 778 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2195 GCAGTGCCTGC
BstYI 1476 GGATCC [GA]^GATC[CT]
2488 GGATCT
Bsu36I 2136 CCTTAGG CC^T[ACGT]AGG
3274 CCTTAGG
DraI 91 TTTAAA TTT^AAA
3676 TTTAAA
DraIII 938 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
1067 CACCTGGTG
DrdI 791 GACTTGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1559 GACCTTGCAGTC
EarI 1766 CTCTTC CTCTTC(1/4)
2478 GAAGAG
Eco81I 2136 CCTTAGG CC^T[ACGT]AGG
3274 CCTTAGG
Hin1I 2457 GACGCC G[GA]^CG[CT]C
2897 GACGCC
Ksp632I 1766 CTCTTC CTCTTC(1/4)
2478 GAAGAG
MflI 1476 GGATCC [GA]^GATC[CT]
2488 GGATCT
NheI 241 GCTAGC G^CTAGC
434 GCTAGC
RcaI 700 TCATGA T^CATGA
1830 TCATGA
SphI 275 GCATGC GCATG^C
2544 GCATGC
SspBI 631 TGTACA T^GTACA
1806 TGTACA
StuI 2430 AGGCCT AGG^CCT
2768 AGGCCT
XhoII 1476 GGATCC [GA]^GATC[CT]
2488 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI