##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI AscI AseI AsnI
Asp718I AspEI AvrII BbsI BcgI
BciVI BfaI BfrI BglII BlnI
BpuAI BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BsrDI BsrGI
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DrdI Eam1105I EcoRI EcoRV
EcoT22I HincII HindII HpaI KpnI
MaeI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI SexAI SfiI
SfuI SnaBI SpeI Sse8387I SspBI
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AgeI 2032 ACCGGT A^CCGGT
Alw44I 2025 GTGCAC G^TGCAC
AocI 943 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2194 AGCGCT AGC^GCT
ApaLI 2025 GTGCAC G^TGCAC
Asp700I 1736 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1084 TGGCCA TGG^CCA
BamHI 407 GGATCC G^GATCC
BbrPI 1404 CACGTG CAC^GTG
BbvCI 696 GCTGAGG CCTCAGC(-5/-2)
BclI 474 TGATCA T^GATCA
BsaAI 1404 CACGTG [CT]AC^GT[GA]
BsmBI 56 CGTCTC CGTCTC(1/5)
BsmI 1836 GAATGC GAATGC(1/-1)
BstAPI 1346 GCACCGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 407 GGATCC [GA]^GATC[CT]
Bsu36I 943 CCTCAGG CC^T[ACGT]AGG
DraI 345 TTTAAA TTT^AAA
DraIII 1347 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 795 CTCTTC CTCTTC(1/4)
Eco47III 2194 AGCGCT AGC^GCT
Eco81I 943 CCTCAGG CC^T[ACGT]AGG
FbaI 474 TGATCA T^GATCA
FseI 1206 GGCCGGCC GGCCGG^CC
HindIII 630 AAGCTT A^AGCTT
Ksp632I 795 CTCTTC CTCTTC(1/4)
KspI 3038 CCGCGG CCGC^GG
MflI 407 GGATCC [GA]^GATC[CT]
MluNI 1084 TGGCCA TGG^CCA
MscI 1084 TGGCCA TGG^CCA
NcoI 1787 CCATGG C^CATGG
PflMI 1087 CCACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2032 ACCGGT A^CCGGT
PmaCI 1404 CACGTG CAC^GTG
PmlI 1404 CACGTG CAC^GTG
SacII 3038 CCGCGG CCGC^GG
SapI 794 GCTCTTC GCTCTTC(1/4)
SgrAI 2219 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1503 CTCAAG C^T[CT][GA]AG
Van91I 1087 CCACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 407 GGATCC [GA]^GATC[CT]
XmnI 1736 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 580 GAATTT [GA]^AATT[CT]
1739 GAATTT
AlwNI 2154 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2823 CAGTCGCTG
ApoI 580 GAATTT [GA]^AATT[CT]
1739 GAATTT
BbeI 2116 GGCGCC GGCGC^C
2550 GGCGCC
BlpI 2 GCTCAGC GC^T[ACGT]AGC
2365 GCTGAGC
Bpu1102I 2 GCTCAGC GC^T[ACGT]AGC
2365 GCTGAGC
BspLU11I 954 ACATGT A^CATGT
1327 ACATGT
BsrBI 279 CCGCTC CCGCTC(-3/-3)
149 GAGCGG
BssHII 1342 GCGCGC G^CGCGC
2287 GCGCGC
BstXI 1123 CCAGCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1213 CCACGGTCATGG
CelII 2 GCTCAGC GC^T[ACGT]AGC
2365 GCTGAGC
EagI 89 CGGCCG C^GGCCG
1205 CGGCCG
EclXI 89 CGGCCG C^GGCCG
1205 CGGCCG
Eco52I 89 CGGCCG C^GGCCG
1205 CGGCCG
EcoNI 398 CCTCATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2768 CCTGTGCCAGG
KasI 2116 GGCGCC G^GCGCC
2550 GGCGCC
NarI 2116 GGCGCC GG^CGCC
2550 GGCGCC
PleI 2560 GACTC GAGTC(4/5)
707 GAGTC
PvuII 1898 CAGCTG CAG^CTG
2154 CAGCTG
ScaI 1369 AGTACT AGT^ACT
1594 AGTACT
SfoI 2116 GGCGCC GGC^GCC
2550 GGCGCC
SphI 1534 GCATGC GCATG^C
1649 GCATGC
StuI 1294 AGGCCT AGG^CCT
2882 AGGCCT
XcmI 736 CCAGGGCTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1027 CCAACCCGGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI ApaI AspHI AspI
AvaI AvaII AviII BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PpuMI PshAI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XmaCI XmaI