##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AatII       AflII       AgeI        AocI        AscI        
AseI        AsnI        AviII       BbvCI       BciVI       
BfrI        BglII       BsaBI       BsiEI       BsiWI       
BspDI       BspLU11I    BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     Bsu36I      ClaI        CpoI        
DrdI        EagI        EclXI       Eco52I      Eco81I      
FspI        HincII      HindII      HindIII     HpaI        
KspI        MamI        MluI        NdeI        NheI        
NotI        NruI        NspI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshBI       PvuI        
RsrII       SacII       SalI        SbfI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SphI        
Sse8387I    SspI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       3312  GGTACC               G^GTACC
AccI         3584  GTCTAC               GT^[AC][GT]AC
AclI         2880  AACGTT               AA^CGTT
AcsI         2489  GAATTC               [GA]^AATT[CT]
AhdI         1823  GACTGTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1937  GTGCAC               G^TGCAC
Aor51HI      3534  AGCGCT               AGC^GCT
ApaLI        1937  GTGCAC               G^TGCAC
ApoI         2489  GAATTC               [GA]^AATT[CT]
Asp718I      3312  GGTACC               G^GTACC
AspEI        1823  GACTGTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        1590  CCTAGG               C^CTAGG
BclI          789  TGATCA               T^GATCA
BlnI         1590  CCTAGG               C^CTAGG
BsaI          216  GAGACC               GGTCTC(1/5)
BsgI          798  CTGCAC               GTGCAG(16/14)
BsmI         2793  GAATGC               GAATGC(1/-1)
Bsp1407I      275  TGTACA               T^GTACA
BsrBI         365  GAGCGG               CCGCTC(-3/-3)
BsrGI         275  TGTACA               T^GTACA
BstAPI       3022  GCAGACCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        499  GGTGACC              G^GT[ACGT]ACC
BstPI         499  GGTGACC              G^GT[ACGT]ACC
DraIII        676  CACTACGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     1823  GACTGTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     3534  AGCGCT               AGC^GCT
EcoO65I       499  GGTGACC              G^GT[ACGT]ACC
EcoRI        2489  GAATTC               G^AATTC
EcoRV        1140  GATATC               GAT^ATC
EcoT22I      2948  ATGCAT               ATGCA^T
FbaI          789  TGATCA               T^GATCA
KpnI         3312  GGTACC               GGTAC^C
MfeI          591  CAATTG               C^AATTG
MunI          591  CAATTG               C^AATTG
NsiI         2948  ATGCAT               ATGCA^T
PshAI         460  GACGTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     2880  AACGTT               AA^CGTT
SacI         3457  GAGCTC               GAGCT^C
SapI         1969  GCTCTTC              GCTCTTC(1/4)
ScaI         2530  AGTACT               AGT^ACT
SpeI          706  ACTAGT               A^CTAGT
SspBI         275  TGTACA               T^GTACA
StuI         2989  AGGCCT               AGG^CCT
XbaI         2146  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII         403  CGCG                 CG^CG
             1453  CGCG                
AccIII       3223  TCCGGA               T^CCGGA
             3547  TCCGGA              
AcyI         1998  GGCGCC               G[GA]^CG[CT]C
             2729  GGCGCC              
BamHI        1011  GGATCC               G^GATCC
             2153  GGATCC              
BbeI         1998  GGCGCC               GGCGC^C
             2729  GGCGCC              
BcgI          411  CGAGCGACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              429  CGAAGAGACTGC        
BglI         2021  GCCGCAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2162  GCCACACTGGC         
BlpI          164  GCTGAGC              GC^T[ACGT]AGC
             2421  GCTGAGC             
Bpu1102I      164  GCTGAGC              GC^T[ACGT]AGC
             2421  GCTGAGC             
BsaHI        1998  GGCGCC               G[GA]^CG[CT]C
             2729  GGCGCC              
BseAI        3223  TCCGGA               T^CCGGA
             3547  TCCGGA              
BspEI        3223  TCCGGA               T^CCGGA
             3547  TCCGGA              
BspHI        1158  TCATGA               T^CATGA
             3111  TCATGA              
BsrDI         952  CATTGC               GCAATG(2/0)
             1039  CATTGC              
BstUI         403  CGCG                 CG^CG
             1453  CGCG                
BstXI         855  CCAACATTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1044  CCAGTCATGTGG        
CelII         164  GCTGAGC              GC^T[ACGT]AGC
             2421  GCTGAGC             
DraI         1606  TTTAAA               TTT^AAA
             3562  TTTAAA              
EcoNI        1246  CCTGCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1942  CCTGCCCCAGG         
FseI         1255  GGCCGGCC             GGCCGG^CC
             2170  GGCCGGCC            
Hin1I        1998  GGCGCC               G[GA]^CG[CT]C
             2729  GGCGCC              
KasI         1998  GGCGCC               G^GCGCC
             2729  GGCGCC              
MroI         3223  TCCGGA               T^CCGGA
             3547  TCCGGA              
MvnI          403  CGCG                 CG^CG
             1453  CGCG                
NarI         1998  GGCGCC               GG^CGCC
             2729  GGCGCC              
NcoI         2687  CCATGG               C^CATGG
             2922  CCATGG              
RcaI         1158  TCATGA               T^CATGA
             3111  TCATGA              
SfoI         1998  GGCGCC               GGC^GCC
             2729  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AciI        AfaI        AflIII      AluI        AlwI        
AlwNI       ApaI        Asp700I     AspHI       AspI        
AvaI        AvaII       BalI        BanI        BanII       
BbrPI       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaAI       BsaJI       
BsaWI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
PflFI       PflMI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XcmI        XhoII       XmaCI       
XmaI        XmnI