##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccI AccIII AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspEI AviII
BamHI BbrPI BclI BfrI BglII
BsaAI BsaBI BseAI BsiWI BspDI
BspEI BspHI BspLU11I Bst1107I BstBI
BstZ17I ClaI Eam1105I Eco47III EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII KspI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
PvuI PvuII RcaI SacII SalI
SbfI SfiI SfuI SnaBI SpeI
SphI Sse8387I StuI SwaI TaqI
TthHB8I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 602 GGTACC G^GTACC
AclI 1027 AACGTT AA^CGTT
AlwNI 44 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 602 GGTACC G^GTACC
AvrII 2136 CCTAGG C^CTAGG
BbsI 877 GTCTTC GAAGAC(2/6)
BcgI 2167 CGATCCTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2733 GTATCC GTATCC(6/5)
BlnI 2136 CCTAGG C^CTAGG
BlpI 2884 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2884 GCTGAGC GC^T[ACGT]AGC
BpuAI 877 GTCTTC GAAGAC(2/6)
BsaWI 627 TCCGGT [AT]^CCGG[AT]
BsiEI 23 CGGCCG CG[GA][CT]^CG
BsmI 3064 GAATGC GAATGC(1/-1)
BssSI 445 CTCGTG CACGAG(-5/-1)
BstEII 1083 GGTTACC G^GT[ACGT]ACC
BstPI 1083 GGTTACC G^GT[ACGT]ACC
BstYI 588 GGATCT [GA]^GATC[CT]
CelII 2884 GCTGAGC GC^T[ACGT]AGC
CpoI 629 CGGTCCG CG^G[AT]CCG
DraIII 820 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 23 CGGCCG C^GGCCG
EclXI 23 CGGCCG C^GGCCG
Eco52I 23 CGGCCG C^GGCCG
EcoO65I 1083 GGTTACC G^GT[ACGT]ACC
KpnI 602 GGTACC GGTAC^C
MaeII 1028 ACGT A^CGT
MflI 588 GGATCT [GA]^GATC[CT]
NcoI 2728 CCATGG C^CATGG
NspI 2441 GCATGT [GA]CATG^[CT]
PshAI 2818 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1027 AACGTT AA^CGTT
RsrII 629 CGGTCCG CG^G[AT]CCG
SacI 247 GAGCTC GAGCT^C
ScaI 3371 AGTACT AGT^ACT
SexAI 821 ACCAGGT A^CC[AT]GGT
SspI 3302 AATATT AAT^ATT
XcmI 3238 CCATAAAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 588 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 1362 AAATTT [GA]^AATT[CT]
1661 AAATTT
ApaI 705 GGGCCC GGGCC^C
2723 GGGCCC
ApoI 1362 AAATTT [GA]^AATT[CT]
1661 AAATTT
AspHI 247 GAGCTC G[AT]GC[AT]^C
2940 GAGCAC
AspI 1753 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2116 GACAGGGTC
BalI 1939 TGGCCA TGG^CCA
2662 TGGCCA
BsiHKAI 247 GAGCTC G[AT]GC[AT]^C
2940 GAGCAC
BsmBI 1801 CGTCTC CGTCTC(1/5)
1751 GAGACG
Bsp1407I 1062 TGTACA T^GTACA
3036 TGTACA
BspMI 810 GCAGGT ACCTGC(4/8)
836 GCAGGT
BsrDI 994 CATTGC GCAATG(2/0)
1565 GCAATG
BsrGI 1062 TGTACA T^GTACA
3036 TGTACA
BsrI 911 ACTGG ACTGG(1/-1)
2665 CCAGT
DrdI 48 GACTGCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
532 GACGCGCTTGTC
EcoNI 337 CCTCAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1989 CCTCCCAAAGG
HincII 1173 GTTAAC GT[CT]^[GA]AC
1453 GTTAAC
HindII 1173 GTTAAC GT[CT]^[GA]AC
1453 GTTAAC
HpaI 1173 GTTAAC GTT^AAC
1453 GTTAAC
MluNI 1939 TGGCCA TGG^CCA
2662 TGGCCA
MscI 1939 TGGCCA TGG^CCA
2662 TGGCCA
MslI 1285 CATAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2624 CACTCTGGTG
PflFI 1753 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2116 GACAGGGTC
PflMI 819 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3238 CCATAAAGTGG
PpuMI 578 GGGTCCT [GA]G^G[AT]CC[CT]
638 GGGTCCC
SapI 1108 GAAGAGC GCTCTTC(1/4)
567 GCTCTTC
SgrAI 75 CGCCGGCG C[GA]^CCGG[CT]G
452 CGCCGGCG
SmlI 2034 CTCAAG C^T[CT][GA]AG
2161 CTCAAG
SspBI 1062 TGTACA T^GTACA
3036 TGTACA
Tth111I 1753 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2116 GACAGGGTC
Van91I 819 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3238 CCATAAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AocI Asp700I AvaI AvaII
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XmaCI XmaI XmnI