##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
AviII AvrII BbrPI BbvCI BciVI
BclI BfrI BlnI BsaBI BseAI
BsiWI BsmI Bsp1407I BspEI BspHI
BsrDI BsrGI Bst1107I BstBI BstZ17I
DraI DrdI EcoRV EcoT22I FbaI
FspI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RcaI SacI SalI ScaI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI Van91I XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 981 GGTACC G^GTACC
AccI 1903 GTCTAC GT^[AC][GT]AC
AflIII 1775 ACATGT A^C[GA][CT]GT
AhdI 305 GACTCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 981 GGTACC G^GTACC
AspEI 305 GACTCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1845 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1526 GGATCC G^GATCC
BglII 264 AGATCT A^GATCT
BsaAI 2572 TACGTG [CT]AC^GT[GA]
BsaWI 2904 TCCGGT [AT]^CCGG[AT]
BspDI 2563 ATCGAT AT^CGAT
BspLU11I 1775 ACATGT A^CATGT
BstAPI 1224 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2412 GGTGACC G^GT[ACGT]ACC
BstPI 2412 GGTGACC G^GT[ACGT]ACC
ClaI 2563 ATCGAT AT^CGAT
CpoI 1058 CGGACCG CG^G[AT]CCG
DraIII 2202 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 305 GACTCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2412 GGTGACC G^GT[ACGT]ACC
EcoRI 2536 GAATTC G^AATTC
FseI 2364 GGCCGGCC GGCCGG^CC
HindIII 1183 AAGCTT A^AGCTT
HpaI 2494 GTTAAC GTT^AAC
KpnI 981 GGTACC GGTAC^C
MseI 2495 TTAA T^TAA
NspI 1775 ACATGT [GA]CATG^[CT]
PaeR7I 1582 CTCGAG C^TCGAG
PflFI 1845 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 138 CGATCG CGAT^CG
RsrII 1058 CGGACCG CG^G[AT]CCG
SapI 1797 GAAGAGC GCTCTTC(1/4)
SbfI 272 CCTGCAGG CCTGCA^GG
SexAI 1139 ACCTGGT A^CC[AT]GGT
SmaI 226 CCCGGG CCC^GGG
Sse8387I 272 CCTGCAGG CCTGCA^GG
Tru9I 2495 TTAA T^TAA
Tth111I 1845 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1582 CTCGAG C^TCGAG
XmaCI 226 CCCGGG C^CCGGG
XmaI 226 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 1552 AAATTC [GA]^AATT[CT]
2536 GAATTC
AocI 1111 CCTGAGG CC^T[ACGT]AGG
2468 CCTTAGG
ApoI 1552 AAATTC [GA]^AATT[CT]
2536 GAATTC
Asp700I 319 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
825 GAACTACTTC
AspHI 112 GTGCTC G[AT]GC[AT]^C
3213 GTGCTC
BfaI 2150 CTAG C^TAG
2790 CTAG
BsiHKAI 112 GTGCTC G[AT]GC[AT]^C
3213 GTGCTC
BsrBI 1267 CCGCTC CCGCTC(-3/-3)
2278 GAGCGG
BssSI 109 CTCGTG CACGAG(-5/-1)
3253 CTCGTG
BstXI 122 CCAACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1433 CCAGCATGATGG
Bsu36I 1111 CCTGAGG CC^T[ACGT]AGG
2468 CCTTAGG
Eco81I 1111 CCTGAGG CC^T[ACGT]AGG
2468 CCTTAGG
HincII 2494 GTTAAC GT[CT]^[GA]AC
3053 GTTGAC
HindII 2494 GTTAAC GT[CT]^[GA]AC
3053 GTTGAC
MaeI 2150 CTAG C^TAG
2790 CTAG
NaeI 671 GCCGGC GCC^GGC
2365 GCCGGC
NgoMIV 671 GCCGGC G^CCGGC
2365 GCCGGC
PvuII 1720 CAGCTG CAG^CTG
2440 CAGCTG
SfiI 156 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2043 GGCCCCGGTGGCC
Tsp509I 1553 AATT ^AATT
2537 AATT
XcmI 542 CCAGCTTCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2501 CCACGGTGGCTGTGG
XmnI 319 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
825 GAACTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcgI BcnI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XhoII