##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
AocI Aor51HI AseI AsnI Asp718I
AspEI BamHI BbeI BbrPI BbvCI
BclI BglII BsaAI BsaWI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BsrBI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI DraIII Eam1105I
Eco47III Eco81I EcoO65I EcoRV EcoT22I
FbaI HindIII HpaI KasI KpnI
MfeI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII SapI
SbfI ScaI SfiI SfoI SfuI
SnaBI SpeI SphI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 2495 GTCGAC GT^[AC][GT]AC
AscI 1691 GGCGCGCC GG^CGCGCC
AspI 2573 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2775 TGCGCA TGC^GCA
AvrII 2599 CCTAGG C^CTAGG
BanI 1479 GGCACC G^G[CT][GA]CC
BcgI 2032 GCATCAAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 763 GTATCC GTATCC(6/5)
BglI 1321 GCCACACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2599 CCTAGG C^CTAGG
BsaBI 862 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1449 GGTCTC GGTCTC(1/5)
Bsp1407I 642 TGTACA T^GTACA
BspLU11I 639 ACATGT A^CATGT
BsrDI 340 GCAATG GCAATG(2/0)
BsrGI 642 TGTACA T^GTACA
BssSI 1701 CACGAG CACGAG(-5/-1)
CpoI 1909 CGGACCG CG^G[AT]CCG
DraI 2822 TTTAAA TTT^AAA
DrdI 1430 GACGGTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2065 CGGCCG C^GGCCG
EclXI 2065 CGGCCG C^GGCCG
Eco52I 2065 CGGCCG C^GGCCG
EcoRI 174 GAATTC G^AATTC
FseI 1128 GGCCGGCC GGCCGG^CC
FspI 2775 TGCGCA TGC^GCA
HincII 2495 GTCGAC GT[CT]^[GA]AC
HindII 2495 GTCGAC GT[CT]^[GA]AC
MamI 862 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 2573 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1819 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1443 GACGACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 1909 CGGACCG CG^G[AT]CCG
SalI 2495 GTCGAC G^TCGAC
SgrAI 1311 CACCGGCG C[GA]^CCGG[CT]G
SspBI 642 TGTACA T^GTACA
Tsp45I 441 GTCAC ^GT[GC]AC
Tth111I 2573 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 477 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1761 GACGTC GACGT^C
2498 GACGTC
AflII 161 CTTAAG C^TTAAG
2439 CTTAAG
Alw44I 1308 GTGCAC G^TGCAC
1367 GTGCAC
AlwNI 347 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1818 CAGGTCCTG
ApaI 2312 GGGCCC GGGCC^C
2695 GGGCCC
ApaLI 1308 GTGCAC G^TGCAC
1367 GTGCAC
Asp700I 195 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2045 GAAGGAGTTC
BalI 994 TGGCCA TGG^CCA
1528 TGGCCA
BfrI 161 CTTAAG C^TTAAG
2439 CTTAAG
BsiEI 1723 CGGTCG CG[GA][CT]^CG
2065 CGGCCG
BspHI 254 TCATGA T^CATGA
2715 TCATGA
BspMI 844 ACCTGC ACCTGC(4/8)
2056 ACCTGC
BssHII 1510 GCGCGC G^CGCGC
1692 GCGCGC
BstYI 2138 AGATCC [GA]^GATC[CT]
2254 GGATCT
EcoNI 1015 CCTTTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1850 CCTGGCCGAGG
KspI 753 CCGCGG CCGC^GG
1940 CCGCGG
MaeIII 441 GTCAC ^GT[ACGT]AC
1151 GTTAC
MflI 2138 AGATCC [GA]^GATC[CT]
2254 GGATCT
MluI 1363 ACGCGT A^CGCGT
2481 ACGCGT
MluNI 994 TGGCCA TGG^CCA
1528 TGGCCA
MscI 994 TGGCCA TGG^CCA
1528 TGGCCA
MslI 1005 CACCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2298 CATATTAGTG
NcoI 809 CCATGG C^CATGG
1063 CCATGG
NspI 639 ACATGT [GA]CATG^[CT]
1408 ACATGC
PaeR7I 2040 CTCGAG C^TCGAG
2429 CTCGAG
PflMI 667 CCAGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1817 CCAGGTCCTGG
PstI 2582 CTGCAG CTGCA^G
2606 CTGCAG
RcaI 254 TCATGA T^CATGA
2715 TCATGA
SacII 753 CCGCGG CCGC^GG
1940 CCGCGG
SexAI 1300 ACCTGGT A^CC[AT]GGT
1816 ACCAGGT
SfcI 2582 CTGCAG C^T[GA][CT]AG
2606 CTGCAG
StuI 1807 AGGCCT AGG^CCT
2602 AGGCCT
Van91I 667 CCAGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1817 CCAGGTCCTGG
XcmI 1483 CCAGCGACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2466 CCAGGGGTGGGGTGG
XhoI 2040 CTCGAG C^TCGAG
2429 CTCGAG
XhoII 2138 AGATCC [GA]^GATC[CT]
2254 GGATCT
XmnI 195 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2045 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XmaCI
XmaI