##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BcgI BciVI BfaI
BfrI BlnI BseAI BsiWI BsmI
BspEI BspHI BspLU11I BssHII Bst1107I
BstBI BstZ17I CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoT22I
FseI HincII HindII HindIII HpaI
KpnI KspI MaeI MfeI MluI
MroI MunI NheI NotI NruI
NsiI NspV PacI PflFI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AgeI 1585 ACCGGT A^CCGGT
AviII 1869 TGCGCA TGC^GCA
BbrPI 1226 CACGTG CAC^GTG
BclI 1780 TGATCA T^GATCA
BglI 1282 GCCTGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1904 AGATCT A^GATCT
BsaBI 1682 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 876 CGGTCG CG[GA][CT]^CG
BspDI 1109 ATCGAT AT^CGAT
BssSI 1031 CACGAG CACGAG(-5/-1)
BstAPI 1594 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1234 GGTCACC G^GT[ACGT]ACC
BstPI 1234 GGTCACC G^GT[ACGT]ACC
ClaI 1109 ATCGAT AT^CGAT
DraI 362 TTTAAA TTT^AAA
DraIII 1726 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1234 GGTCACC G^GT[ACGT]ACC
EcoRV 688 GATATC GAT^ATC
FbaI 1780 TGATCA T^GATCA
FspI 1869 TGCGCA TGC^GCA
MamI 1682 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2092 GCCGGC GCC^GGC
NcoI 1572 CCATGG C^CATGG
NdeI 737 CATATG CA^TATG
NgoMIV 2092 GCCGGC G^CCGGC
NspI 1560 GCATGT [GA]CATG^[CT]
PaeR7I 1913 CTCGAG C^TCGAG
PinAI 1585 ACCGGT A^CCGGT
PmaCI 1226 CACGTG CAC^GTG
PmlI 1226 CACGTG CAC^GTG
PshAI 2619 GACGTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 185 CAGCTG CAG^CTG
SapI 2342 GAAGAGC GCTCTTC(1/4)
SbfI 2213 CCTGCAGG CCTGCA^GG
SmaI 1754 CCCGGG CCC^GGG
Sse8387I 2213 CCTGCAGG CCTGCA^GG
SspI 64 AATATT AAT^ATT
StuI 1328 AGGCCT AGG^CCT
XhoI 1913 CTCGAG C^TCGAG
XmaCI 1754 CCCGGG C^CCGGG
XmaI 1754 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 434 GACGTC GACGT^C
2619 GACGTC
Alw44I 488 GTGCAC G^TGCAC
1028 GTGCAC
AocI 1765 CCTCAGG CC^T[ACGT]AGG
2270 CCTGAGG
ApaLI 488 GTGCAC G^TGCAC
1028 GTGCAC
BbeI 141 GGCGCC GGCGC^C
2095 GGCGCC
BbvCI 2561 CCTCAGC CCTCAGC(-5/-2)
1513 GCTGAGG
BlpI 199 GCTGAGC GC^T[ACGT]AGC
1402 GCTGAGC
Bpu1102I 199 GCTGAGC GC^T[ACGT]AGC
1402 GCTGAGC
BsaAI 806 TACGTG [CT]AC^GT[GA]
1226 CACGTG
BsaI 828 GAGACC GGTCTC(1/5)
1774 GGTCTC
BsmBI 1370 CGTCTC CGTCTC(1/5)
884 GAGACG
Bsp1407I 726 TGTACA T^GTACA
2553 TGTACA
BspMI 1221 ACCTGC ACCTGC(4/8)
2782 ACCTGC
BsrBI 1088 CCGCTC CCGCTC(-3/-3)
1442 GAGCGG
BsrDI 1186 CATTGC GCAATG(2/0)
2128 CATTGC
BsrGI 726 TGTACA T^GTACA
2553 TGTACA
Bsu36I 1765 CCTCAGG CC^T[ACGT]AGG
2270 CCTGAGG
CelII 199 GCTGAGC GC^T[ACGT]AGC
1402 GCTGAGC
DrdI 1790 GACTCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2166 GACTGCTGGGTC
Eco81I 1765 CCTCAGG CC^T[ACGT]AGG
2270 CCTGAGG
EcoNI 1543 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2182 CCTGAATCAGG
EcoT14I 588 CCAAGG C^C[AT][AT]GG
1572 CCATGG
HaeII 141 GGCGCC [GA]GCGC^[CT]
2095 GGCGCC
KasI 141 GGCGCC G^GCGCC
2095 GGCGCC
MspA1I 185 CAGCTG C[AC]G^C[GT]G
1454 CAGCGG
NarI 141 GGCGCC GG^CGCC
2095 GGCGCC
PflMI 1462 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1975 CCAGAGTGTGG
SacI 1405 GAGCTC GAGCT^C
2468 GAGCTC
SfoI 141 GGCGCC GGC^GCC
2095 GGCGCC
SspBI 726 TGTACA T^GTACA
2553 TGTACA
StyI 588 CCAAGG C^C[AT][AT]GG
1572 CCATGG
Van91I 1462 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1975 CCAGAGTGTGG
XcmI 466 CCAGGTTCTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
814 CCACAGTGGCATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII