##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AhdI AocI Aor51HI
AscI AseI AsnI AspEI AviII
AvrII BamHI BglI BlnI BsiWI
Bsp1407I BspDI BsrGI BssHII Bsu36I
ClaI CpoI DrdI Eam1105I Eco47III
Eco81I EcoNI EcoRV FseI FspI
KspI MfeI MluI MunI NcoI
NotI NruI PacI PflMI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
TfiI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 69 enzymes) #
###################################
Acc65I 4385 GGTACC G^GTACC
AccII 2779 CGCG CG^CG
AccIII 4469 TCCGGA T^CCGGA
AflII 4654 CTTAAG C^TTAAG
AflIII 4011 ACATGT A^C[GA][CT]GT
AgeI 1265 ACCGGT A^CCGGT
Asp700I 3369 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4385 GGTACC G^GTACC
AspI 2707 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2765 TGGCCA TGG^CCA
BbrPI 3430 CACGTG CAC^GTG
BciVI 2686 GGATAC GTATCC(6/5)
BclI 1763 TGATCA T^GATCA
BfrI 4654 CTTAAG C^TTAAG
BglII 3474 AGATCT A^GATCT
BlpI 1800 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1800 GCTGAGC GC^T[ACGT]AGC
BsaAI 3430 CACGTG [CT]AC^GT[GA]
BsaBI 4473 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4469 TCCGGA T^CCGGA
BsgI 3541 CTGCAC GTGCAG(16/14)
BsmBI 1228 GAGACG CGTCTC(1/5)
BspEI 4469 TCCGGA T^CCGGA
BspHI 2742 TCATGA T^CATGA
BspLU11I 4011 ACATGT A^CATGT
BsrBI 301 GAGCGG CCGCTC(-3/-3)
BssSI 1545 CACGAG CACGAG(-5/-1)
Bst1107I 830 GTATAC GTA^TAC
BstEII 1161 GGTGACC G^GT[ACGT]ACC
BstPI 1161 GGTGACC G^GT[ACGT]ACC
BstUI 2779 CGCG CG^CG
BstZ17I 830 GTATAC GTA^TAC
CelII 1800 GCTGAGC GC^T[ACGT]AGC
EcoO65I 1161 GGTGACC G^GT[ACGT]ACC
EcoT22I 2125 ATGCAT ATGCA^T
FbaI 1763 TGATCA T^GATCA
HincII 3805 GTTAAC GT[CT]^[GA]AC
HindII 3805 GTTAAC GT[CT]^[GA]AC
HpaI 3805 GTTAAC GTT^AAC
KpnI 4385 GGTACC GGTAC^C
MamI 4473 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2765 TGGCCA TGG^CCA
MroI 4469 TCCGGA T^CCGGA
MscI 2765 TGGCCA TGG^CCA
MvnI 2779 CGCG CG^CG
NaeI 2918 GCCGGC GCC^GGC
NdeI 2845 CATATG CA^TATG
NgoMIV 2918 GCCGGC G^CCGGC
NheI 906 GCTAGC G^CTAGC
NsiI 2125 ATGCAT ATGCA^T
PaeR7I 1390 CTCGAG C^TCGAG
PflFI 2707 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1265 ACCGGT A^CCGGT
PmaCI 3430 CACGTG CAC^GTG
PmlI 3430 CACGTG CAC^GTG
PpuMI 2150 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1145 GACACCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2742 TCATGA T^CATGA
SacI 3846 GAGCTC GAGCT^C
SapI 3550 GAAGAGC GCTCTTC(1/4)
ScaI 1532 AGTACT AGT^ACT
SexAI 1493 ACCTGGT A^CC[AT]GGT
StuI 2380 AGGCCT AGG^CCT
SwaI 4419 ATTTAAAT ATTT^AAAT
Tth111I 2707 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 444 TCTAGA T^CTAGA
XcmI 2606 CCAGTGGCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1390 CTCGAG C^TCGAG
XmnI 3369 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 830 GTATAC GT^[AC][GT]AC
1075 GTCTAC
ApaI 19 GGGCCC GGGCC^C
3465 GGGCCC
BbeI 1258 GGCGCC GGCGC^C
2611 GGCGCC
BbvCI 1795 CCTCAGC CCTCAGC(-5/-2)
1168 GCTGAGG
BsaI 3724 GAGACC GGTCTC(1/5)
1497 GGTCTC
BsmI 4215 GAATGC GAATGC(1/-1)
703 GCATTC
BspMI 3640 ACCTGC ACCTGC(4/8)
4050 GCAGGT
BstBI 233 TTCGAA TT^CGAA
661 TTCGAA
BstXI 1934 CCAGCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1988 CCACACTGATGG
DraI 3409 TTTAAA TTT^AAA
4420 TTTAAA
EagI 1958 CGGCCG C^GGCCG
2677 CGGCCG
EclXI 1958 CGGCCG C^GGCCG
2677 CGGCCG
Eco52I 1958 CGGCCG C^GGCCG
2677 CGGCCG
EcoT14I 24 CCAAGG C^C[AT][AT]GG
1781 CCAAGG
HindIII 3370 AAGCTT A^AGCTT
4657 AAGCTT
KasI 1258 GGCGCC G^GCGCC
2611 GGCGCC
NarI 1258 GGCGCC GG^CGCC
2611 GGCGCC
NspV 233 TTCGAA TT^CGAA
661 TTCGAA
SfoI 1258 GGCGCC GGC^GCC
2611 GGCGCC
SfuI 233 TTCGAA TT^CGAA
661 TTCGAA
SphI 2123 GCATGC GCATG^C
2958 GCATGC
StyI 24 CCAAGG C^C[AT][AT]GG
1781 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcgI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII