##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AgeI        AhdI        AlwNI       AocI        Aor51HI     
ApaI        AscI        AspEI       AvaI        AviII       
AvrII       BalI        BamHI       BanII       BbeI        
BcgI        BclI        BcnI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       BspDI       BspEI       
BsrFI       BssHII      BssSI       BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraII       DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRV       FbaI        
FseI        FspI        HaeII       HapII       HhaI        
Hin1I       HinP1I      HindIII     HpaI        HpaII       
KasI        KspI        MflI        MluI        MluNI       
MroI        MscI        MspI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PaeR7I      PinAI       
PmeI        PpuMI       PshAI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    StuI        
XbaI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I       3753  GGTACC               G^GTACC
AflII        2199  CTTAAG               C^TTAAG
AlwI         3150  GGATC                GGATC(4/5)
Asp718I      3753  GGTACC               G^GTACC
AspI         3480  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        3433  CACGTG               CAC^GTG
BbvCI        3644  GCTGAGG              CCTCAGC(-5/-2)
BfrI         2199  CTTAAG               C^TTAAG
BsaAI        3433  CACGTG               [CT]AC^GT[GA]
BsaBI        3775  GATTCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1979  GAGACC               GGTCTC(1/5)
Bsp1407I     2124  TGTACA               T^GTACA
BsrBI         881  GAGCGG               CCGCTC(-3/-3)
BsrGI        2124  TGTACA               T^GTACA
Bst1107I     2655  GTATAC               GTA^TAC
BstAPI       1846  GCATGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      2655  GTATAC               GTA^TAC
DsaI         1759  CCATGG               C^C[GA][CT]GG
EcoRI        3192  GAATTC               G^AATTC
EcoT22I      2986  ATGCAT               ATGCA^T
FauI         2010  CCCGC                CCCGC(4/6)
HgaI         2152  GACGC                GACGC(5/10)
KpnI         3753  GGTACC               GGTAC^C
MamI         3775  GATTCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2945  CAATTG               C^AATTG
MspA1I        532  CAGCTG               C[AC]G^C[GT]G
MunI         2945  CAATTG               C^AATTG
NcoI         1759  CCATGG               C^CATGG
NsiI         2986  ATGCAT               ATGCA^T
PacI         2616  TTAATTAA             TTAAT^TAA
PflFI        3480  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        3433  CACGTG               CAC^GTG
PmlI         3433  CACGTG               CAC^GTG
PstI         3253  CTGCAG               CTGCA^G
PvuII         532  CAGCTG               CAG^CTG
ScaI         3297  AGTACT               AGT^ACT
SfcI         3253  CTGCAG               C^T[GA][CT]AG
SphI         2988  GCATGC               GCATG^C
SspBI        2124  TGTACA               T^GTACA
SwaI         3909  ATTTAAAT             ATTT^AAAT
Tsp45I       3394  GTGAC                ^GT[GC]AC
Tth111I      3480  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          943  CCAAAAATCTAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

Alw44I       3430  GTGCAC               G^TGCAC
             3845  GTGCAC              
ApaLI        3430  GTGCAC               G^TGCAC
             3845  GTGCAC              
AspHI        3430  GTGCAC               G[AT]GC[AT]^C
             3845  GTGCAC              
AvaII        2488  GGTCC                G^G[AT]CC
             2603  GGACC               
BanI         2430  GGCACC               G^G[CT][GA]CC
             3753  GGTACC              
BbvI          531  GCAGC                GCAGC(8/12)
             2846  GCTGC               
BpmI         3488  CTCCAG               CTGGAG(16/14)
             3047  CTGGAG              
BsiHKAI      3430  GTGCAC               G[AT]GC[AT]^C
             3845  GTGCAC              
BsrDI         297  CATTGC               GCAATG(2/0)
             1252  CATTGC              
MaeII        1555  ACGT                 A^CGT
             3434  ACGT                
PflMI        2420  CCATCACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2971  CCATAAAATGG         
SapI          878  GAAGAGC              GCTCTTC(1/4)
             1383  GAAGAGC             
SmlI         1733  CTTGAG               C^T[CT][GA]AG
             2199  CTTAAG              
TseI          531  GCAGC                G^C[AT]GC
             2846  GCTGC               
Van91I       2420  CCATCACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2971  CCATAAAATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        ApoI        AseI        AsnI        Asp700I     
BbsI        BciVI       BfaI        BmyI        BpuAI       
BsaJI       BseRI       BsiYI       BslI        BsmAI       
Bsp1286I    BspHI       BspLU11I    BspMI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        EarI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HincII      
HindII      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MslI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PshBI       
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SspI        StyI        TaqI        TfiI        
Tru9I       Tsp509I     TspRI       TthHB8I     XmnI