##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AgeI        
Alw44I      ApaLI       AscI        AspI        AvaII       
AviII       AvrII       BbeI        BbrPI       BbvCI       
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BspDI       BspEI       BsrBI       
BsrDI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     CelII       ClaI        
CpoI        DraIII      DrdI        DsaI        EagI        
EclXI       Eco52I      EcoO65I     EcoT14I     FauI        
FseI        FspI        Hin1I       HpaI        KasI        
KspI        MluI        MroI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspI        StuI        
StyI        SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

Acc65I        269  GGTACC               G^GTACC
AccII         932  CGCG                 CG^CG
AhdI         2205  GACGAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        2603  CAGGTACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          680  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      3392  AGCGCT               AGC^GCT
Asp718I       269  GGTACC               G^GTACC
AspEI        2205  GACGAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1772  TGGCCA               TGG^CCA
BcgI         2527  GCAACACTATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         945  GGATAC               GTATCC(6/5)
BclI         1577  TGATCA               T^GATCA
BsaBI         519  GATAAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1960  GAGACC               GGTCTC(1/5)
BsaWI         949  ACCGGA               [AT]^CCGG[AT]
BstAPI        737  GCAGGGTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         932  CGCG                 CG^CG
Bsu36I        680  CCTGAGG              CC^T[ACGT]AGG
Eam1105I     2205  GACGAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     3392  AGCGCT               AGC^GCT
Eco81I        680  CCTGAGG              CC^T[ACGT]AGG
FbaI         1577  TGATCA               T^GATCA
HaeII        3392  AGCGCT               [GA]GCGC^[CT]
KpnI          269  GGTACC               GGTAC^C
MamI          519  GATAAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1772  TGGCCA               TGG^CCA
MscI         1772  TGGCCA               TGG^CCA
MspA1I       2083  CAGCTG               C[AC]G^C[GT]G
MvnI          932  CGCG                 CG^CG
NaeI          812  GCCGGC               GCC^GGC
NgoMIV        812  GCCGGC               G^CCGGC
PflMI         256  CCACCACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII        2083  CAGCTG               CAG^CTG
SapI         1939  GCTCTTC              GCTCTTC(1/4)
Van91I        256  CCACCACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AccI         1212  GTAGAC               GT^[AC][GT]AC
             1524  GTAGAC              
AflII        1110  CTTAAG               C^TTAAG
             1871  CTTAAG              
AflIII       1116  ACATGT               A^C[GA][CT]GT
             3101  ACATGT              
ApaI         1799  GGGCCC               GGGCC^C
             2342  GGGCCC              
AseI         1718  ATTAAT               AT^TAAT
             2705  ATTAAT              
AsnI         1718  ATTAAT               AT^TAAT
             2705  ATTAAT              
BamHI         401  GGATCC               G^GATCC
              893  GGATCC              
BfrI         1110  CTTAAG               C^TTAAG
             1871  CTTAAG              
BpmI          308  CTCCAG               CTGGAG(16/14)
              475  CTGGAG              
BspHI          73  TCATGA               T^CATGA
             2951  TCATGA              
BspLU11I     1116  ACATGT               A^CATGT
             3101  ACATGT              
BspMI         326  ACCTGC               ACCTGC(4/8)
             1454  ACCTGC              
BsrFI         812  GCCGGC               [GA]^CCGG[CT]
             1047  ACCGGC              
CfoI         2129  GCGC                 GCG^C
             3393  GCGC                
Cfr10I        812  GCCGGC               [GA]^CCGG[CT]
             1047  ACCGGC              
DraII        1799  GGGCCCT              [GA]G^G[ACGT]CC[CT]
             2342  GGGCCCT             
EaeI         1049  CGGCCA               [CT]^GGCC[GA]
             1772  TGGCCA              
EcoO109I     1799  GGGCCCT              [GA]G^G[ACGT]CC[CT]
             2342  GGGCCCT             
EcoRV        1017  GATATC               GAT^ATC
             3885  GATATC              
EcoT22I      3408  ATGCAT               ATGCA^T
             3695  ATGCAT              
HgaI         1008  GACGC                GACGC(5/10)
             2075  GCGTC               
HhaI         2129  GCGC                 GCG^C
             3393  GCGC                
HinP1I       2129  GCGC                 G^CGC
             3393  GCGC                
HincII       3366  GTCAAC               GT[CT]^[GA]AC
             3506  GTTGAC              
HindII       3366  GTCAAC               GT[CT]^[GA]AC
             3506  GTTGAC              
NsiI         3408  ATGCAT               ATGCA^T
             3695  ATGCAT              
PleI         2303  GACTC                GAGTC(4/5)
             2674  GAGTC               
PshBI        1718  ATTAAT               AT^TAAT
             2705  ATTAAT              
PstI         2386  CTGCAG               CTGCA^G
             3957  CTGCAG              
RcaI           73  TCATGA               T^CATGA
             2951  TCATGA              
SacI          305  GAGCTC               GAGCT^C
             3355  GAGCTC              
SexAI        1391  ACCAGGT              A^CC[AT]GGT
             2601  ACCAGGT             
SmaI         1754  CCCGGG               CCC^GGG
             2044  CCCGGG              
SphI         1967  GCATGC               GCATG^C
             3808  GCATGC              
Tsp45I        166  GTGAC                ^GT[GC]AC
             3950  GTGAC               
XcmI          256  CCACCACTTGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              324  CCACCTGCCTACTGG     
XmaCI        1754  CCCGGG               C^CCGGG
             2044  CCCGGG              
XmaI         1754  CCCGGG               C^CCGGG
             2044  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       AvaI        BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpuAI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
EarI        EcoNI       EcoRI       EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MfeI        
MflI        MnlI        MseI        MslI        MspI        
MunI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     XhoII       XmnI