##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AccIII AclI AflIII AhdI
AocI Aor51HI AspEI BamHI BcgI
BglII BlpI Bpu1102I BsaBI BsaI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BsrBI BssSI BstBI
Bsu36I CelII ClaI CpoI DraIII
DrdI Eam1105I Eco47III Eco81I EcoRI
FseI HpaI MamI MluI MroI
NdeI NruI NspV PacI PaeR7I
PmeI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SnaBI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 2522 GGTACC G^GTACC
AflII 2153 CTTAAG C^TTAAG
AgeI 1545 ACCGGT A^CCGGT
Alw44I 173 GTGCAC G^TGCAC
ApaI 373 GGGCCC GGGCC^C
ApaLI 173 GTGCAC G^TGCAC
Asp700I 3835 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2522 GGTACC G^GTACC
AspI 969 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1124 TGCGCA TGC^GCA
AvrII 3830 CCTAGG C^CTAGG
BalI 420 TGGCCA TGG^CCA
BbeI 84 GGCGCC GGCGC^C
BbrPI 1508 CACGTG CAC^GTG
BbvCI 642 CCTCAGC CCTCAGC(-5/-2)
BfrI 2153 CTTAAG C^TTAAG
BlnI 3830 CCTAGG C^CTAGG
BsmI 3453 GAATGC GAATGC(1/-1)
Bsp1407I 2367 TGTACA T^GTACA
BsrGI 2367 TGTACA T^GTACA
Bst1107I 3333 GTATAC GTA^TAC
BstEII 1788 GGTCACC G^GT[ACGT]ACC
BstPI 1788 GGTCACC G^GT[ACGT]ACC
BstXI 2331 CCACAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 846 AGATCC [GA]^GATC[CT]
BstZ17I 3333 GTATAC GTA^TAC
EcoNI 1839 CCTATGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1788 GGTCACC G^GT[ACGT]ACC
EcoRV 863 GATATC GAT^ATC
EcoT22I 2775 ATGCAT ATGCA^T
FspI 1124 TGCGCA TGC^GCA
HincII 2749 GTCAAC GT[CT]^[GA]AC
HindII 2749 GTCAAC GT[CT]^[GA]AC
KasI 84 GGCGCC G^GCGCC
KpnI 2522 GGTACC GGTAC^C
MfeI 2926 CAATTG C^AATTG
MflI 846 AGATCC [GA]^GATC[CT]
MluNI 420 TGGCCA TGG^CCA
MscI 420 TGGCCA TGG^CCA
MunI 2926 CAATTG C^AATTG
NaeI 653 GCCGGC GCC^GGC
NarI 84 GGCGCC GG^CGCC
NcoI 317 CCATGG C^CATGG
NgoMIV 653 GCCGGC G^CCGGC
NotI 1 GCGGCCGC GC^GGCCGC
NsiI 2775 ATGCAT ATGCA^T
PflFI 969 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3508 CCAGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1545 ACCGGT A^CCGGT
PmaCI 1508 CACGTG CAC^GTG
PmlI 1508 CACGTG CAC^GTG
SfoI 84 GGCGCC GGC^GCC
SgrAI 678 CGCCGGTG C[GA]^CCGG[CT]G
SphI 1890 GCATGC GCATG^C
SspBI 2367 TGTACA T^GTACA
StuI 3441 AGGCCT AGG^CCT
Tth111I 969 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3508 CCAGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3121 TCTAGA T^CTAGA
XcmI 1267 CCACGCGAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 846 AGATCC [GA]^GATC[CT]
XmnI 3835 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1808 GTCTAC GT^[AC][GT]AC
3333 GTATAC
AcyI 61 GGCGTC G[GA]^CG[CT]C
84 GGCGCC
AlwI 742 GATCC GGATC(4/5)
847 GATCC
AscI 99 GGCGCGCC GG^CGCGCC
756 GGCGCGCC
AseI 2782 ATTAAT AT^TAAT
3698 ATTAAT
AsnI 2782 ATTAAT AT^TAAT
3698 ATTAAT
BciVI 580 GGATAC GTATCC(6/5)
3021 GTATCC
BclI 1696 TGATCA T^GATCA
2930 TGATCA
BglI 53 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
76 GCCGGGGAGGC
BsaAI 1193 TACGTG [CT]AC^GT[GA]
1508 CACGTG
BsaHI 61 GGCGTC G[GA]^CG[CT]C
84 GGCGCC
BsaWI 1545 ACCGGT [AT]^CCGG[AT]
1800 ACCGGA
EarI 549 CTCTTC CTCTTC(1/4)
2690 GAAGAG
EcoT14I 317 CCATGG C^C[AT][AT]GG
3830 CCTAGG
FbaI 1696 TGATCA T^GATCA
2930 TGATCA
HaeII 84 GGCGCC [GA]GCGC^[CT]
1343 AGCGCC
HgaI 3232 GACGC GACGC(5/10)
62 GCGTC
Hin1I 61 GGCGTC G[GA]^CG[CT]C
84 GGCGCC
Ksp632I 549 CTCTTC CTCTTC(1/4)
2690 GAAGAG
NheI 2096 GCTAGC G^CTAGC
3301 GCTAGC
PshAI 3232 GACGCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3933 GACACTGGTC
PshBI 2782 ATTAAT AT^TAAT
3698 ATTAAT
SacI 1076 GAGCTC GAGCT^C
1085 GAGCTC
SmaI 294 CCCGGG CCC^GGG
2035 CCCGGG
SpeI 2761 ACTAGT A^CTAGT
3610 ACTAGT
StyI 317 CCATGG C^C[AT][AT]GG
3830 CCTAGG
TfiI 1826 GAATC G^A[AT]TC
3543 GATTC
XmaCI 294 CCCGGG C^CCGGG
2035 CCCGGG
XmaI 294 CCCGGG C^CCGGG
2035 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI KspI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I