##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII AgeI AscI AseI AsnI
AviII AvrII BamHI BciVI BclI
BlnI BsaBI BsmI BspDI BspHI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DrdI FbaI FspI HindIII
HpaI MamI MluI NotI NruI
NspV PacI PinAI PmeI PshBI
PvuI RcaI RsrII SalI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI StuI SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 3679 GGTACC G^GTACC
AccI 983 GTCTAC GT^[AC][GT]AC
AclI 4691 AACGTT AA^CGTT
AflII 4174 CTTAAG C^TTAAG
AhdI 1995 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 291 CCTGAGG CC^T[ACGT]AGG
Aor51HI 523 AGCGCT AGC^GCT
Asp700I 1076 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3679 GGTACC G^GTACC
AspEI 1995 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3051 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 3077 CGAAGAGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4174 CTTAAG C^TTAAG
BglII 3175 AGATCT A^GATCT
BlpI 1482 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1482 GCTCAGC GC^T[ACGT]AGC
BsiWI 2197 CGTACG C^GTACG
BstAPI 1459 GCAAGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2651 GGTGACC G^GT[ACGT]ACC
BstPI 2651 GGTGACC G^GT[ACGT]ACC
Bsu36I 291 CCTGAGG CC^T[ACGT]AGG
CelII 1482 GCTCAGC GC^T[ACGT]AGC
DraIII 3315 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1995 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 523 AGCGCT AGC^GCT
Eco81I 291 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2651 GGTGACC G^GT[ACGT]ACC
EcoRV 407 GATATC GAT^ATC
EcoT22I 2722 ATGCAT ATGCA^T
HincII 91 GTCAAC GT[CT]^[GA]AC
HindII 91 GTCAAC GT[CT]^[GA]AC
KpnI 3679 GGTACC GGTAC^C
MfeI 4592 CAATTG C^AATTG
MunI 4592 CAATTG C^AATTG
NdeI 4396 CATATG CA^TATG
NheI 4086 GCTAGC G^CTAGC
NsiI 2722 ATGCAT ATGCA^T
PaeR7I 2775 CTCGAG C^TCGAG
PflFI 3051 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1839 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 2096 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4691 AACGTT AA^CGTT
SbfI 3350 CCTGCAGG CCTGCA^GG
Sse8387I 3350 CCTGCAGG CCTGCA^GG
SspI 4322 AATATT AAT^ATT
Tth111I 3051 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 114 TCTAGA T^CTAGA
XcmI 2098 CCAACGTCCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2775 CTCGAG C^TCGAG
XmnI 1076 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 1190 TCCGGA T^CCGGA
2116 TCCGGA
Alw44I 1223 GTGCAC G^TGCAC
3924 GTGCAC
ApaLI 1223 GTGCAC G^TGCAC
3924 GTGCAC
BbeI 2796 GGCGCC GGCGC^C
3666 GGCGCC
BbrPI 610 CACGTG CAC^GTG
1736 CACGTG
BbvCI 2710 CCTCAGC CCTCAGC(-5/-2)
2938 CCTCAGC
BglI 1410 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3447 GCCGCGGCGGC
BsaAI 610 CACGTG [CT]AC^GT[GA]
1736 CACGTG
BsaI 4431 GAGACC GGTCTC(1/5)
2834 GGTCTC
BseAI 1190 TCCGGA T^CCGGA
2116 TCCGGA
Bsp1407I 802 TGTACA T^GTACA
2068 TGTACA
BspEI 1190 TCCGGA T^CCGGA
2116 TCCGGA
BspLU11I 19 ACATGT A^CATGT
2510 ACATGT
BsrDI 2725 CATTGC GCAATG(2/0)
1767 GCAATG
BsrGI 802 TGTACA T^GTACA
2068 TGTACA
EcoNI 2282 CCTCGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2675 CCTCTCGGAGG
EcoRI 683 GAATTC G^AATTC
991 GAATTC
FseI 2438 GGCCGGCC GGCCGG^CC
3032 GGCCGGCC
KasI 2796 GGCGCC G^GCGCC
3666 GGCGCC
MroI 1190 TCCGGA T^CCGGA
2116 TCCGGA
NarI 2796 GGCGCC GG^CGCC
3666 GGCGCC
PmaCI 610 CACGTG CAC^GTG
1736 CACGTG
PmlI 610 CACGTG CAC^GTG
1736 CACGTG
SacI 3618 GAGCTC GAGCT^C
3749 GAGCTC
SapI 48 GAAGAGC GCTCTTC(1/4)
2274 GAAGAGC
SfoI 2796 GGCGCC GGC^GCC
3666 GGCGCC
SmaI 2452 CCCGGG CCC^GGG
2823 CCCGGG
SphI 2174 GCATGC GCATG^C
3627 GCATGC
SspBI 802 TGTACA T^GTACA
2068 TGTACA
XmaCI 2452 CCCGGG C^CCGGG
2823 CCCGGG
XmaI 2452 CCCGGG C^CCGGG
2823 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII