##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AviII
AvrII BbeI BfrI BlnI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FseI FspI HgaI
Hin1I HpaI KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SphI Sse8387I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 3187 GTCTAC GT^[AC][GT]AC
AclI 104 AACGTT AA^CGTT
Alw44I 1515 GTGCAC G^TGCAC
ApaLI 1515 GTGCAC G^TGCAC
AspI 1632 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 3014 GGTGCC G^G[CT][GA]CC
BbrPI 1840 CACGTG CAC^GTG
BcgI 144 CGACATTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3462 GGATAC GTATCC(6/5)
BclI 2674 TGATCA T^GATCA
BglI 3017 GCCAAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1840 CACGTG [CT]AC^GT[GA]
BsaBI 984 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 894 CTCCTC GAGGAG(10/8)
BsrBI 2212 GAGCGG CCGCTC(-3/-3)
CfoI 2195 GCGC GCG^C
DrdI 3653 GACATGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1310 CCACGG C^C[GA][CT]GG
FbaI 2674 TGATCA T^GATCA
HaeII 2194 AGCGCC [GA]GCGC^[CT]
HhaI 2195 GCGC GCG^C
HinP1I 2195 GCGC G^CGC
HincII 2065 GTCAAC GT[CT]^[GA]AC
HindII 2065 GTCAAC GT[CT]^[GA]AC
MamI 984 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 466 CACTAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 1289 CATATG CA^TATG
PflFI 1632 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1840 CACGTG CAC^GTG
PmlI 1840 CACGTG CAC^GTG
PpuMI 1138 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 104 AACGTT AA^CGTT
SacI 3149 GAGCTC GAGCT^C
SapI 3264 GCTCTTC GCTCTTC(1/4)
SmaI 1374 CCCGGG CCC^GGG
SpeI 467 ACTAGT A^CTAGT
StuI 3174 AGGCCT AGG^CCT
SwaI 4688 ATTTAAAT ATTT^AAAT
Tth111I 1632 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 9 CCACAGAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1374 CCCGGG C^CCGGG
XmaI 1374 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 1839 ACACGT A^C[GA][CT]GT
1841 ACGTGT
AspHI 1515 GTGCAC G[AT]GC[AT]^C
3149 GAGCTC
AvaI 1374 CCCGGG C^[CT]CG[GA]G
3281 CCCGAG
BalI 3808 TGGCCA TGG^CCA
4281 TGGCCA
BamHI 1325 GGATCC G^GATCC
2557 GGATCC
BbvCI 511 CCTCAGC CCTCAGC(-5/-2)
2603 CCTCAGC
BglII 1151 AGATCT A^GATCT
2859 AGATCT
BsiHKAI 1515 GTGCAC G[AT]GC[AT]^C
3149 GAGCTC
BsoBI 1374 CCCGGG C^[CT]CG[GA]G
3281 CCCGAG
BstXI 268 CCAAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2198 CCAGGTGACTGG
DraII 1138 AGGTCCT [GA]G^G[ACGT]CC[CT]
2599 AGGCCCT
EcoNI 3303 CCTCCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3517 CCTCTAGTAGG
EcoO109I 1138 AGGTCCT [GA]G^G[ACGT]CC[CT]
2599 AGGCCCT
FauI 1960 CCCGC CCCGC(4/6)
70 GCGGG
MaeII 105 ACGT A^CGT
1841 ACGT
MluNI 3808 TGGCCA TGG^CCA
4281 TGGCCA
MscI 3808 TGGCCA TGG^CCA
4281 TGGCCA
PflMI 371 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
487 CCAGCAATTGG
PstI 250 CTGCAG CTGCA^G
2786 CTGCAG
ScaI 1096 AGTACT AGT^ACT
3546 AGTACT
Van91I 371 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
487 CCAGCAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AvaII BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI EaeI EarI EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MfeI MflI MnlI MseI MspA1I
MspI MunI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI