##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AflIII      AhdI        AscI        AseI        AsnI        
Asp700I     AspEI       BbeI        BbrPI       BcgI        
BclI        BfrI        BsaAI       BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoNI       EcoRI       FbaI        FseI        
HpaI        KasI        KspI        MluI        MroI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       Sse8387I    SspBI       
SwaI        XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I       3811  GGTACC               G^GTACC
AcyI           11  GACGCC               G[GA]^CG[CT]C
AgeI         3664  ACCGGT               A^CCGGT
AocI           47  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      3817  AGCGCT               AGC^GCT
Asp718I      3811  GGTACC               G^GTACC
AspI          199  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1757  TGCGCA               TGC^GCA
AvrII        3747  CCTAGG               C^CTAGG
BamHI        1364  GGATCC               G^GATCC
BciVI         812  GTATCC               GTATCC(6/5)
BglII        1025  AGATCT               A^GATCT
BlnI         3747  CCTAGG               C^CTAGG
BsaHI          11  GACGCC               G[GA]^CG[CT]C
BsaWI        3664  ACCGGT               [AT]^CCGG[AT]
BsmI         1788  GCATTC               GAATGC(1/-1)
BsrDI        3680  GCAATG               GCAATG(2/0)
BssSI        1918  CACGAG               CACGAG(-5/-1)
BstAPI        328  GCACCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        450  GGTAACC              G^GT[ACGT]ACC
BstPI         450  GGTAACC              G^GT[ACGT]ACC
Bsu36I         47  CCTCAGG              CC^T[ACGT]AGG
DraI          399  TTTAAA               TTT^AAA
Eco47III     3817  AGCGCT               AGC^GCT
Eco81I         47  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       450  GGTAACC              G^GT[ACGT]ACC
EcoRV        2131  GATATC               GAT^ATC
EcoT22I       214  ATGCAT               ATGCA^T
FauI         1086  GCGGG                CCCGC(4/6)
FspI         1757  TGCGCA               TGC^GCA
HaeII        3817  AGCGCT               [GA]GCGC^[CT]
Hin1I          11  GACGCC               G[GA]^CG[CT]C
HindIII       692  AAGCTT               A^AGCTT
KpnI         3811  GGTACC               GGTAC^C
NsiI          214  ATGCAT               ATGCA^T
PflFI         199  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        3664  ACCGGT               A^CCGGT
SacI         3533  GAGCTC               GAGCT^C
SapI         2903  GCTCTTC              GCTCTTC(1/4)
ScaI         3037  AGTACT               AGT^ACT
Tth111I       199  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII        1394  CGCG                 CG^CG
             1999  CGCG                
AcsI         1011  AAATTC               [GA]^AATT[CT]
             4601  AAATTT              
ApoI         1011  AAATTC               [GA]^AATT[CT]
             4601  AAATTT              
BglI         1120  GCCACTAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1729  GCCAGTGAGGC         
BlpI          481  GCTCAGC              GC^T[ACGT]AGC
             2727  GCTTAGC             
Bpu1102I      481  GCTCAGC              GC^T[ACGT]AGC
             2727  GCTTAGC             
BsaBI        1252  GATGACTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1467  GATCCCCATC          
BseRI        1684  CTCCTC               GAGGAG(10/8)
             2255  GAGGAG              
BsmFI         346  GTCCC                GGGAC(10/14)
             1864  GTCCC               
BsrFI         272  ACCGGC               [GA]^CCGG[CT]
             3664  ACCGGT              
BstUI        1394  CGCG                 CG^CG
             1999  CGCG                
BstXI          74  CCAGAAAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              555  CCATGAGGGTGG        
CelII         481  GCTCAGC              GC^T[ACGT]AGC
             2727  GCTTAGC             
Cfr10I        272  ACCGGC               [GA]^CCGG[CT]
             3664  ACCGGT              
HincII        268  GTCAAC               GT[CT]^[GA]AC
             2242  GTCAAC              
HindII        268  GTCAAC               GT[CT]^[GA]AC
             2242  GTCAAC              
MaeII        1202  ACGT                 A^CGT
             2240  ACGT                
MamI         1252  GATGACTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1467  GATCCCCATC          
MfeI          635  CAATTG               C^AATTG
              869  CAATTG              
MunI          635  CAATTG               C^AATTG
              869  CAATTG              
MvnI         1394  CGCG                 CG^CG
             1999  CGCG                
SmaI          141  CCCGGG               CCC^GGG
             1295  CCCGGG              
SmlI         1213  CTTGAG               C^T[CT][GA]AG
             1942  CTCAAG              
SpeI         2488  ACTAGT               A^CTAGT
             3934  ACTAGT              
SphI         1951  GCATGC               GCATG^C
             2525  GCATGC              
SspI          622  AATATT               AAT^ATT
             1520  AATATT              
XmaCI         141  CCCGGG               C^CCGGG
             1295  CCCGGG              
XmaI          141  CCCGGG               C^CCGGG
             1295  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AciI        AfaI        AluI        Alw44I      AlwI        
AlwNI       ApaI        ApaLI       AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII