##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AclI AgeI AhdI AocI
ApaI AscI AspEI BbeI BbrPI
BcgI BcnI BsaAI BsaBI BsiEI
BsiWI BspDI BsrDI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoT22I FseI
HpaI KasI KspI MamI MfeI
MluI MunI NarI NciI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 4774 GGTACC G^GTACC
AccII 2009 CGCG CG^CG
AccIII 2554 TCCGGA T^CCGGA
AflII 1789 CTTAAG C^TTAAG
Aor51HI 2579 AGCGCT AGC^GCT
AseI 319 ATTAAT AT^TAAT
AsnI 319 ATTAAT AT^TAAT
Asp700I 519 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4774 GGTACC G^GTACC
AspI 1957 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 787 CCCGAG C^[CT]CG[GA]G
AviII 1973 TGCGCA TGC^GCA
AvrII 3265 CCTAGG C^CTAGG
BamHI 2405 GGATCC G^GATCC
BciVI 3215 GGATAC GTATCC(6/5)
BclI 3489 TGATCA T^GATCA
BfrI 1789 CTTAAG C^TTAAG
BglII 2532 AGATCT A^GATCT
BlnI 3265 CCTAGG C^CTAGG
BlpI 104 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 104 GCTGAGC GC^T[ACGT]AGC
BsaWI 2554 TCCGGA [AT]^CCGG[AT]
BseAI 2554 TCCGGA T^CCGGA
BsmBI 1183 GAGACG CGTCTC(1/5)
BsoBI 787 CCCGAG C^[CT]CG[GA]G
BspEI 2554 TCCGGA T^CCGGA
BspHI 959 TCATGA T^CATGA
BsrBI 930 CCGCTC CCGCTC(-3/-3)
BssSI 2712 CACGAG CACGAG(-5/-1)
BstAPI 2057 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2009 CGCG CG^CG
CelII 104 GCTGAGC GC^T[ACGT]AGC
DrdI 2786 GACAGGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2579 AGCGCT AGC^GCT
EcoRI 665 GAATTC G^AATTC
FbaI 3489 TGATCA T^GATCA
FspI 1973 TGCGCA TGC^GCA
KpnI 4774 GGTACC GGTAC^C
MaeII 2516 ACGT A^CGT
MroI 2554 TCCGGA T^CCGGA
MvnI 2009 CGCG CG^CG
PflFI 1957 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 2049 GTTTAAAC GTTT^AAAC
PshBI 319 ATTAAT AT^TAAT
RcaI 959 TCATGA T^CATGA
SacI 382 GAGCTC GAGCT^C
SphI 3357 GCATGC GCATG^C
StuI 1606 AGGCCT AGG^CCT
Tth111I 1957 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 519 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 1185 GACGCC G[GA]^CG[CT]C
3596 GGCGTC
Alw44I 907 GTGCAC G^TGCAC
4003 GTGCAC
ApaLI 907 GTGCAC G^TGCAC
4003 GTGCAC
BalI 3518 TGGCCA TGG^CCA
4330 TGGCCA
BanI 1660 GGCACC G^G[CT][GA]CC
4774 GGTACC
BbvCI 1889 CCTCAGC CCTCAGC(-5/-2)
3308 GCTGAGG
BsaHI 1185 GACGCC G[GA]^CG[CT]C
3596 GGCGTC
BsaI 1955 GAGACC GGTCTC(1/5)
2172 GGTCTC
BseRI 1443 CTCCTC GAGGAG(10/8)
3311 GAGGAG
BsmI 393 GCATTC GAATGC(1/-1)
1635 GCATTC
Bsp1407I 2264 TGTACA T^GTACA
4172 TGTACA
BsrFI 275 GCCGGC [GA]^CCGG[CT]
3544 GCCGGC
BsrGI 2264 TGTACA T^GTACA
4172 TGTACA
BstXI 1481 CCACTACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2989 CCAGGAACCTGG
Cfr10I 275 GCCGGC [GA]^CCGG[CT]
3544 GCCGGC
DraIII 1199 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2515 CACGTTGTG
EcoRV 1390 GATATC GAT^ATC
1807 GATATC
HaeII 1317 GGCGCT [GA]GCGC^[CT]
2579 AGCGCT
HgaI 1185 GACGC GACGC(5/10)
3597 GCGTC
Hin1I 1185 GACGCC G[GA]^CG[CT]C
3596 GGCGTC
HincII 412 GTTGAC GT[CT]^[GA]AC
4087 GTCAAC
HindII 412 GTTGAC GT[CT]^[GA]AC
4087 GTCAAC
HindIII 337 AAGCTT A^AGCTT
989 AAGCTT
MluNI 3518 TGGCCA TGG^CCA
4330 TGGCCA
MscI 3518 TGGCCA TGG^CCA
4330 TGGCCA
NaeI 275 GCCGGC GCC^GGC
3544 GCCGGC
NcoI 3515 CCATGG C^CATGG
4333 CCATGG
NgoMIV 275 GCCGGC G^CCGGC
3544 GCCGGC
PpuMI 1672 AGGTCCC [GA]G^G[AT]CC[CT]
3733 GGGTCCT
PstI 1594 CTGCAG CTGCA^G
1932 CTGCAG
SapI 519 GAAGAGC GCTCTTC(1/4)
2113 GAAGAGC
SspBI 2264 TGTACA T^GTACA
4172 TGTACA
SspI 2540 AATATT AAT^ATT
3441 AATATT
TaqI 1695 TCGA T^CGA
4424 TCGA
TthHB8I 1695 TCGA T^CGA
4424 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BanII BbsI BbvI BfaI
BglI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SpeI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII