##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccI AccIII Aor51HI ApaI
AscI AseI AsnI AviII BamHI
BglII BsaBI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoRI EcoRV FseI
FspI HpaI MamI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PmeI PshBI PvuI
RsrII SalI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 1389 GGTACC G^GTACC
AcyI 1672 GGCGCC G[GA]^CG[CT]C
AgeI 256 ACCGGT A^CCGGT
AhdI 2130 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3567 GTGCAC G^TGCAC
ApaLI 3567 GTGCAC G^TGCAC
Asp718I 1389 GGTACC G^GTACC
AspEI 2130 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4634 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2707 CCTAGG C^CTAGG
BbeI 1672 GGCGCC GGCGC^C
BclI 2690 TGATCA T^GATCA
BlnI 2707 CCTAGG C^CTAGG
BsaHI 1672 GGCGCC G[GA]^CG[CT]C
BsaI 535 GAGACC GGTCTC(1/5)
BsmI 4054 GCATTC GAATGC(1/-1)
Bsp1407I 4227 TGTACA T^GTACA
BsrBI 1754 CCGCTC CCGCTC(-3/-3)
BsrGI 4227 TGTACA T^GTACA
BstYI 3468 GGATCT [GA]^GATC[CT]
Eam1105I 2130 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2690 TGATCA T^GATCA
HaeII 1672 GGCGCC [GA]GCGC^[CT]
Hin1I 1672 GGCGCC G[GA]^CG[CT]C
HindIII 4257 AAGCTT A^AGCTT
KasI 1672 GGCGCC G^GCGCC
KpnI 1389 GGTACC GGTAC^C
MflI 3468 GGATCT [GA]^GATC[CT]
NarI 1672 GGCGCC GG^CGCC
NspI 2357 ACATGC [GA]CATG^[CT]
PflFI 4634 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 256 ACCGGT A^CCGGT
PshAI 868 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 3297 GCTCTTC GCTCTTC(1/4)
SbfI 114 CCTGCAGG CCTGCA^GG
ScaI 1076 AGTACT AGT^ACT
SfiI 19 GGCCCCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1672 GGCGCC GGC^GCC
Sse8387I 114 CCTGCAGG CCTGCA^GG
SspBI 4227 TGTACA T^GTACA
Tth111I 4634 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 3468 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflII 2179 CTTAAG C^TTAAG
3556 CTTAAG
AflIII 1383 ACACGT A^C[GA][CT]GT
4351 ACACGT
AlwI 3468 GGATC GGATC(4/5)
3599 GGATC
AocI 1738 CCTGAGG CC^T[ACGT]AGG
3334 CCTAAGG
Asp700I 1242 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2279 GAAATAATTC
BbrPI 1384 CACGTG CAC^GTG
4352 CACGTG
BbvCI 392 CCTCAGC CCTCAGC(-5/-2)
3150 CCTCAGC
BcgI 552 CGAGATGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1932 GCACCTGGGTCG
BfrI 2179 CTTAAG C^TTAAG
3556 CTTAAG
BglI 20 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
397 GCCTCTATGGC
BsaAI 1384 CACGTG [CT]AC^GT[GA]
4352 CACGTG
BsaWI 256 ACCGGT [AT]^CCGG[AT]
1868 TCCGGT
BsrDI 1394 CATTGC GCAATG(2/0)
4166 CATTGC
BsrFI 256 ACCGGT [GA]^CCGG[CT]
1985 ACCGGC
BstEII 2875 GGTGACC G^GT[ACGT]ACC
3486 GGTCACC
BstPI 2875 GGTGACC G^GT[ACGT]ACC
3486 GGTCACC
Bsu36I 1738 CCTGAGG CC^T[ACGT]AGG
3334 CCTAAGG
Cfr10I 256 ACCGGT [GA]^CCGG[CT]
1985 ACCGGC
Eco81I 1738 CCTGAGG CC^T[ACGT]AGG
3334 CCTAAGG
EcoO65I 2875 GGTGACC G^GT[ACGT]ACC
3486 GGTCACC
EcoT22I 1641 ATGCAT ATGCA^T
2586 ATGCAT
HgaI 211 GACGC GACGC(5/10)
3994 GACGC
HincII 1021 GTCAAC GT[CT]^[GA]AC
2859 GTTGAC
HindII 1021 GTCAAC GT[CT]^[GA]AC
2859 GTTGAC
NsiI 1641 ATGCAT ATGCA^T
2586 ATGCAT
PflMI 123 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3246 CCAAGAAATGG
PmaCI 1384 CACGTG CAC^GTG
4352 CACGTG
PmlI 1384 CACGTG CAC^GTG
4352 CACGTG
PpuMI 1154 AGGACCT [GA]G^G[AT]CC[CT]
2308 GGGACCC
PvuII 641 CAGCTG CAG^CTG
1315 CAGCTG
Van91I 123 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3246 CCAAGAAATGG
XmnI 1242 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2279 GAAATAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AclI AcsI AfaI
AluI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BciVI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI Psp1406I PstI RcaI RsaI
SacI SacII Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XmaCI XmaI