##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AvrII
BamHI BbeI BbvCI BcgI BfrI
BlnI BseAI BsiEI BsiWI Bsp1407I
BspEI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CpoI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I FseI KasI KpnI KspI
MluI MroI NarI NheI NotI
NruI NspV PacI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SexAI SfoI SfuI SgrAI
SmaI SnaBI SspBI SwaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AcyI 1803 GACGCC G[GA]^CG[CT]C
AgeI 490 ACCGGT A^CCGGT
AhdI 2988 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 914 GGGCCC GGGCC^C
AspEI 2988 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 232 TGCGCA TGC^GCA
BglI 1203 GCCATCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2340 AGATCT A^GATCT
BlpI 3159 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3159 GCTGAGC GC^T[ACGT]AGC
BsaBI 644 GATCGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1803 GACGCC G[GA]^CG[CT]C
BsaWI 490 ACCGGT [AT]^CCGG[AT]
BsmI 2373 GAATGC GAATGC(1/-1)
BspDI 1292 ATCGAT AT^CGAT
BspHI 1336 TCATGA T^CATGA
BssHII 2831 GCGCGC G^CGCGC
BssSI 1434 CACGAG CACGAG(-5/-1)
BstYI 2340 AGATCT [GA]^GATC[CT]
CelII 3159 GCTGAGC GC^T[ACGT]AGC
ClaI 1292 ATCGAT AT^CGAT
DraI 2001 TTTAAA TTT^AAA
DraIII 2588 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2988 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1295 GATATC GAT^ATC
EcoT22I 1523 ATGCAT ATGCA^T
FauI 1754 GCGGG CCCGC(4/6)
FspI 232 TGCGCA TGC^GCA
HaeII 1735 AGCGCC [GA]GCGC^[CT]
HgaI 1803 GACGC GACGC(5/10)
Hin1I 1803 GACGCC G[GA]^CG[CT]C
HpaI 3190 GTTAAC GTT^AAC
MamI 644 GATCGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2614 CAATTG C^AATTG
MflI 2340 AGATCT [GA]^GATC[CT]
MunI 2614 CAATTG C^AATTG
NcoI 2329 CCATGG C^CATGG
NdeI 2869 CATATG CA^TATG
NsiI 1523 ATGCAT ATGCA^T
PaeR7I 1778 CTCGAG C^TCGAG
PflMI 1670 CCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 490 ACCGGT A^CCGGT
RcaI 1336 TCATGA T^CATGA
SbfI 1881 CCTGCAGG CCTGCA^GG
ScaI 748 AGTACT AGT^ACT
SfiI 1202 GGCCATCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2034 ACTAGT A^CTAGT
SphI 229 GCATGC GCATG^C
Sse8387I 1881 CCTGCAGG CCTGCA^GG
SspI 3946 AATATT AAT^ATT
StuI 245 AGGCCT AGG^CCT
Van91I 1670 CCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2465 TCTAGA T^CTAGA
XhoI 1778 CTCGAG C^TCGAG
XhoII 2340 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspI 1784 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1895 GACCATGTC
AvaI 1141 CCCGAG C^[CT]CG[GA]G
1778 CTCGAG
BanI 2540 GGTGCC G^G[CT][GA]CC
3707 GGTGCC
BbrPI 131 CACGTG CAC^GTG
182 CACGTG
BbsI 1701 GAAGAC GAAGAC(2/6)
2113 GAAGAC
BclI 1375 TGATCA T^GATCA
3642 TGATCA
BcnI 1445 CCCGG CC^[GC]GG
2027 CCGGG
BpuAI 1701 GAAGAC GAAGAC(2/6)
2113 GAAGAC
BsaAI 131 CACGTG [CT]AC^GT[GA]
182 CACGTG
BsaI 1137 GAGACC GGTCTC(1/5)
1893 GAGACC
BsgI 1796 CTGCAC GTGCAG(16/14)
185 GTGCAG
BsmBI 807 CGTCTC CGTCTC(1/5)
879 CGTCTC
BsoBI 1141 CCCGAG C^[CT]CG[GA]G
1778 CTCGAG
BspLU11I 208 ACATGT A^CATGT
1612 ACATGT
BspMI 3143 ACCTGC ACCTGC(4/8)
1884 GCAGGT
BsrBI 1775 CCGCTC CCGCTC(-3/-3)
1752 GAGCGG
BstAPI 2073 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2268 GCAACACTTGC
BstXI 1723 CCACGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2083 CCAACACCTTGG
DraII 1782 AGGACCT [GA]G^G[ACGT]CC[CT]
2010 AGGTCCC
DrdI 834 GACGGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2133 GACATCTATGTC
EcoO109I 1782 AGGACCT [GA]G^G[ACGT]CC[CT]
2010 AGGTCCC
FbaI 1375 TGATCA T^GATCA
3642 TGATCA
HindIII 2219 AAGCTT A^AGCTT
3032 AAGCTT
NciI 1445 CCCGG CC^[GC]GG
2027 CCGGG
PflFI 1784 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1895 GACCATGTC
PmaCI 131 CACGTG CAC^GTG
182 CACGTG
PmlI 131 CACGTG CAC^GTG
182 CACGTG
PpuMI 1782 AGGACCT [GA]G^G[AT]CC[CT]
2010 AGGTCCC
Tth111I 1784 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1895 GACCATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BalI BanII BbvI
BciVI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI