##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII BanI BanII
BbeI BbrPI BbvI BciVI BclI
BcnI BfrI BglI BglII BsaAI
BsaHI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmFI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrDI BsrGI BssHII
BstAPI BstBI BstEII BstPI BstUI
ClaI CpoI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FbaI Fnu4HI FseI FspI HaeII
HgaI Hin1I HindIII ItaI KasI
KpnI KspI MluI MroI MspA1I
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SspBI SwaI TseI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AocI 3010 CCTCAGG CC^T[ACGT]AGG
AvrII 2822 CCTAGG C^CTAGG
BalI 3288 TGGCCA TGG^CCA
BamHI 2405 GGATCC G^GATCC
BbsI 299 GTCTTC GAAGAC(2/6)
BcgI 3190 CGATTCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2822 CCTAGG C^CTAGG
BlpI 2894 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2894 GCTCAGC GC^T[ACGT]AGC
BpuAI 299 GTCTTC GAAGAC(2/6)
BsaBI 323 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2994 GGTCTC GGTCTC(1/5)
BsaWI 3379 TCCGGT [AT]^CCGG[AT]
BsmBI 2964 GAGACG CGTCTC(1/5)
BspMI 2384 ACCTGC ACCTGC(4/8)
BsrFI 2939 ACCGGC [GA]^CCGG[CT]
BssSI 2761 CACGAG CACGAG(-5/-1)
Bsu36I 3010 CCTCAGG CC^T[ACGT]AGG
CelII 2894 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2939 ACCGGC [GA]^CCGG[CT]
DrdI 1570 GACTTCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 3288 TGGCCA [CT]^GGCC[GA]
EarI 2415 GAAGAG CTCTTC(1/4)
Eco81I 3010 CCTCAGG CC^T[ACGT]AGG
EcoNI 2513 CCTTTAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 2822 CCTAGG C^C[AT][AT]GG
EcoT22I 861 ATGCAT ATGCA^T
FauI 3024 CCCGC CCCGC(4/6)
HpaI 3646 GTTAAC GTT^AAC
Ksp632I 2415 GAAGAG CTCTTC(1/4)
MamI 323 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3288 TGGCCA TGG^CCA
MscI 3288 TGGCCA TGG^CCA
NdeI 2111 CATATG CA^TATG
NsiI 861 ATGCAT ATGCA^T
PleI 5 GAGTC GAGTC(4/5)
PshAI 1143 GACTTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2386 CTGCAG CTGCA^G
SbfI 2385 CCTGCAGG CCTGCA^GG
SfaNI 1405 GCATC GCATC(5/9)
Sse8387I 2385 CCTGCAGG CCTGCA^GG
StyI 2822 CCTAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 3025 CCGC CCGC(-3/-1)
3068 CCGC
AfaI 155 GTAC GT^AC
3491 GTAC
AflIII 467 ACATGT A^C[GA][CT]GT
2093 ACATGT
AvaII 161 GGACC G^G[AT]CC
546 GGACC
BbvCI 3028 CCTCAGC CCTCAGC(-5/-2)
3201 CCTCAGC
BmyI 209 GTGCCC G[AGT]GC[ACT]^C
2313 GGGCAC
BsgI 2833 GTGCAG GTGCAG(16/14)
3139 GTGCAG
Bsp1286I 209 GTGCCC G[AGT]GC[ACT]^C
2313 GGGCAC
BspLU11I 467 ACATGT A^CATGT
2093 ACATGT
BstYI 2405 GGATCC [GA]^GATC[CT]
3753 GGATCT
Cfr13I 161 GGACC G^G[ACGT]CC
546 GGACC
DraII 160 AGGACCT [GA]G^G[ACGT]CC[CT]
545 AGGACCT
EcoO109I 160 AGGACCT [GA]G^G[ACGT]CC[CT]
545 AGGACCT
EcoRV 1818 GATATC GAT^ATC
2971 GATATC
MboII 2415 GAAGA GAAGA(8/7)
300 TCTTC
MfeI 1970 CAATTG C^AATTG
2586 CAATTG
MflI 2405 GGATCC [GA]^GATC[CT]
3753 GGATCT
MunI 1970 CAATTG C^AATTG
2586 CAATTG
MwoI 2812 GCTCTTGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2843 GCAGTCTTGGC
NlaIV 110 GGTTCC GG[ACGT]^[ACGT]CC
2405 GGATCC
PpuMI 160 AGGACCT [GA]G^G[AT]CC[CT]
545 AGGACCT
RsaI 155 GTAC GT^AC
3491 GTAC
Sau96I 161 GGACC G^G[ACGT]CC
546 GGACC
SfcI 33 CTGTAG C^T[GA][CT]AG
2386 CTGCAG
StuI 1218 AGGCCT AGG^CCT
1302 AGGCCT
TaqI 658 TCGA T^CGA
1078 TCGA
TthHB8I 658 TCGA T^CGA
1078 TCGA
XhoII 2405 GGATCC [GA]^GATC[CT]
3753 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccI AcsI AluI AlwI ApoI
Asp700I BfaI BpmI BsaJI BsiYI
BslI BsmAI BsmI BspHI BsrI
BssKI Bst1107I BstF5I BstNI BstXI
BstZ17I Cac8I CfoI DdeI DpnII
DraI EcoRI EcoRII FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI MaeI MaeII
MaeIII MboI MnlI MseI MslI
MspI MvaI NdeII NlaIII NspI
RcaI Sau3AI ScrFI SphI SspI
TfiI Tru9I Tsp45I Tsp509I TspRI
XmnI