##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      ApaLI       AscI        Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BbeI        BciVI       BfrI        BlnI        
BsiWI       BsmBI       BsmI        BspDI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoO65I     EcoRV       
EcoT22I     FseI        FspI        KasI        KpnI        
KspI        MfeI        MluI        MluNI       MscI        
MunI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PshAI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SphI        SwaI        
Tth111I     Van91I      XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccII        1007  CGCG                 CG^CG
AccIII       1487  TCCGGA               T^CCGGA
AcyI         1202  GACGCC               G[GA]^CG[CT]C
AocI         3335  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1379  AGCGCT               AGC^GCT
AseI         1874  ATTAAT               AT^TAAT
AsnI         1874  ATTAAT               AT^TAAT
BanI          102  GGCACC               G^G[CT][GA]CC
BbrPI        3386  CACGTG               CAC^GTG
BcgI         1266  GCAAAGCCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         2999  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2999  GCTCAGC              GC^T[ACGT]AGC
BsaHI        1202  GACGCC               G[GA]^CG[CT]C
BseAI        1487  TCCGGA               T^CCGGA
BsiEI         895  CGGTCG               CG[GA][CT]^CG
Bsp1407I     4360  TGTACA               T^GTACA
BspEI        1487  TCCGGA               T^CCGGA
BspHI        1248  TCATGA               T^CATGA
BspLU11I     3867  ACATGT               A^CATGT
BsrBI         884  GAGCGG               CCGCTC(-3/-3)
BsrFI        1352  GCCGGT               [GA]^CCGG[CT]
BsrGI        4360  TGTACA               T^GTACA
BssSI        2286  CACGAG               CACGAG(-5/-1)
BstAPI        446  GCAAATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1007  CGCG                 CG^CG
Bsu36I       3335  CCTGAGG              CC^T[ACGT]AGG
CelII        2999  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1352  GCCGGT               [GA]^CCGG[CT]
EaeI          955  TGGCCG               [CT]^GGCC[GA]
Eco47III     1379  AGCGCT               AGC^GCT
Eco81I       3335  CCTGAGG              CC^T[ACGT]AGG
EcoRI        2645  GAATTC               G^AATTC
FauI          725  GCGGG                CCCGC(4/6)
HgaI         1202  GACGC                GACGC(5/10)
Hin1I        1202  GACGCC               G[GA]^CG[CT]C
HindIII      3295  AAGCTT               A^AGCTT
HpaI         2270  GTTAAC               GTT^AAC
MroI         1487  TCCGGA               T^CCGGA
MvnI         1007  CGCG                 CG^CG
PmaCI        3386  CACGTG               CAC^GTG
PmlI         3386  CACGTG               CAC^GTG
PshBI        1874  ATTAAT               AT^TAAT
RcaI         1248  TCATGA               T^CATGA
SacI         2354  GAGCTC               GAGCT^C
SbfI         2380  CCTGCAGG             CCTGCA^GG
ScaI         2636  AGTACT               AGT^ACT
SexAI        2521  ACCTGGT              A^CC[AT]GGT
SpeI         2976  ACTAGT               A^CTAGT
Sse8387I     2380  CCTGCAGG             CCTGCA^GG
SspBI        4360  TGTACA               T^GTACA
StuI         1020  AGGCCT               AGG^CCT
TaqI         1418  TCGA                 T^CGA
TthHB8I      1418  TCGA                 T^CGA
XbaI         3895  TCTAGA               T^CTAGA
XcmI          531  CCAGCAAGAGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AlwNI         329  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              421  CAGAAGCTG           
ApaI          385  GGGCCC               GGGCC^C
             3308  GGGCCC              
Asp700I       623  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3532  GAATAATTTC          
BbvCI         520  GCTGAGG              CCTCAGC(-5/-2)
              649  GCTGAGG             
BglII        1086  AGATCT               A^GATCT
             1341  AGATCT              
BsaAI        1178  TACGTG               [CT]AC^GT[GA]
             3386  CACGTG              
BsaWI        1487  TCCGGA               [AT]^CCGG[AT]
             2628  ACCGGA              
BsrDI         922  CATTGC               GCAATG(2/0)
             3797  CATTGC              
BstXI          34  CCACCGCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              363  CCAGTGAGCTGG        
CfoI         1380  GCGC                 GCG^C
             2251  GCGC                
HaeII        1379  AGCGCT               [GA]GCGC^[CT]
             2250  GGCGCT              
HhaI         1380  GCGC                 GCG^C
             2251  GCGC                
HinP1I       1380  GCGC                 G^CGC
             2251  GCGC                
NcoI          912  CCATGG               C^CATGG
             3059  CCATGG              
NspI         1281  ACATGC               [GA]CATG^[CT]
             3867  ACATGT              
PvuII        1424  CAGCTG               CAG^CTG
             3274  CAGCTG              
XmnI          623  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3532  GAATAATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        ApoI        AspHI       AvaI        
AvaII       BanII       BbsI        BbvI        BclI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaBI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FbaI        Fnu4HI      FokI        HaeIII      
HapII       HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XhoII