##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AclI AflII AgeI Aor51HI
AscI Asp718I AviII AvrII BamHI
BbvCI BcgI BciVI BclI BfrI
BlnI BsiWI BspDI BspHI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FbaI
FseI FspI HpaI KpnI MluI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI Psp1406I PvuI
RcaI RsrII SalI SfiI SfuI
SgrAI SnaBI SpeI SspI StuI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 2249 GACGTC GACGT^C
AccI 3812 GTAGAC GT^[AC][GT]AC
AhdI 1088 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1592 GTGCAC G^TGCAC
AocI 193 CCTGAGG CC^T[ACGT]AGG
ApaLI 1592 GTGCAC G^TGCAC
AseI 3043 ATTAAT AT^TAAT
AsnI 3043 ATTAAT AT^TAAT
AspEI 1088 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 915 CCCGGG C^[CT]CG[GA]G
BalI 1371 TGGCCA TGG^CCA
BbeI 59 GGCGCC GGCGC^C
BsaBI 3192 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 322 GAATGC GAATGC(1/-1)
BsoBI 915 CCCGGG C^[CT]CG[GA]G
BsrBI 707 GAGCGG CCGCTC(-3/-3)
BssSI 3161 CACGAG CACGAG(-5/-1)
BstAPI 693 GCAAGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 193 CCTGAGG CC^T[ACGT]AGG
DraIII 3161 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 128 GACATTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1088 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 193 CCTGAGG CC^T[ACGT]AGG
EcoT22I 3416 ATGCAT ATGCA^T
HindIII 2225 AAGCTT A^AGCTT
KasI 59 GGCGCC G^GCGCC
KspI 155 CCGCGG CCGC^GG
MamI 3192 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 291 CAATTG C^AATTG
MluNI 1371 TGGCCA TGG^CCA
MscI 1371 TGGCCA TGG^CCA
MunI 291 CAATTG C^AATTG
NarI 59 GGCGCC GG^CGCC
NsiI 3416 ATGCAT ATGCA^T
PflMI 1693 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 2169 GTTTAAAC GTTT^AAAC
PshAI 2113 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3043 ATTAAT AT^TAAT
SacII 155 CCGCGG CCGC^GG
SapI 80 GCTCTTC GCTCTTC(1/4)
SbfI 532 CCTGCAGG CCTGCA^GG
SfoI 59 GGCGCC GGC^GCC
SmaI 915 CCCGGG CCC^GGG
Sse8387I 532 CCTGCAGG CCTGCA^GG
SwaI 2803 ATTTAAAT ATTT^AAAT
Van91I 1693 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 915 CCCGGG C^CCGGG
XmaI 915 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 498 TCCGGA T^CCGGA
1017 TCCGGA
Asp700I 296 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
865 GAAGAAGTTC
AspI 2730 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3365 GACTCTGTC
BbrPI 1160 CACGTG CAC^GTG
3405 CACGTG
BfaI 2678 CTAG C^TAG
3808 CTAG
BglI 1187 GCCAAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1864 GCCCACCAGGC
BglII 673 AGATCT A^GATCT
2212 AGATCT
BseAI 498 TCCGGA T^CCGGA
1017 TCCGGA
BsiEI 1042 CGACCG CG[GA][CT]^CG
1300 CGACCG
BspEI 498 TCCGGA T^CCGGA
1017 TCCGGA
BspLU11I 2827 ACATGT A^CATGT
3513 ACATGT
BspMI 393 ACCTGC ACCTGC(4/8)
3552 ACCTGC
BstXI 467 CCAAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
927 CCAACCTGGTGG
EcoRI 683 GAATTC G^AATTC
2462 GAATTC
HincII 2630 GTCAAC GT[CT]^[GA]AC
2831 GTTGAC
HindII 2630 GTCAAC GT[CT]^[GA]AC
2831 GTTGAC
MaeI 2678 CTAG C^TAG
3808 CTAG
MroI 498 TCCGGA T^CCGGA
1017 TCCGGA
NaeI 1192 GCCGGC GCC^GGC
1511 GCCGGC
NcoI 720 CCATGG C^CATGG
1698 CCATGG
NgoMIV 1192 GCCGGC G^CCGGC
1511 GCCGGC
PflFI 2730 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3365 GACTCTGTC
PmaCI 1160 CACGTG CAC^GTG
3405 CACGTG
PmlI 1160 CACGTG CAC^GTG
3405 CACGTG
ScaI 333 AGTACT AGT^ACT
2930 AGTACT
SexAI 930 ACCTGGT A^CC[AT]GGT
1089 ACCTGGT
Tth111I 2730 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3365 GACTCTGTC
XcmI 9 CCAAGCCGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1080 CCAGCCCGGACCTGG
XmnI 296 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
865 GAAGAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaII BanI BanII
BbsI BbvI BcnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I Bsp1407I BsrDI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII