##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AflII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI AviII
AvrII BalI BciVI BfrI BglII
BlnI BsaBI BsiWI BsmBI BsmI
Bsp1407I BspDI BspLU11I BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraIII DrdI Eco47III Eco81I EcoO65I
EcoRI EcoT22I FspI HpaI MamI
MfeI MluI MluNI MscI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI PvuI RsrII SacI SbfI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XcmI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3172 GACGTC GACGT^C
Acc65I 2061 GGTACC G^GTACC
AccI 2837 GTCGAC GT^[AC][GT]AC
AccIII 1896 TCCGGA T^CCGGA
AclI 1231 AACGTT AA^CGTT
AflIII 1164 ACGTGT A^C[GA][CT]GT
AhdI 2642 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3062 GGGCCC GGGCC^C
Asp718I 2061 GGTACC G^GTACC
AspEI 2642 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2936 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2053 GGCGCC GGCGC^C
BbrPI 2636 CACGTG CAC^GTG
BlpI 2740 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2740 GCTCAGC GC^T[ACGT]AGC
BseAI 1896 TCCGGA T^CCGGA
BspEI 1896 TCCGGA T^CCGGA
BsrBI 3299 GAGCGG CCGCTC(-3/-3)
BsrDI 2728 CATTGC GCAATG(2/0)
CelII 2740 GCTCAGC GC^T[ACGT]AGC
DraI 3493 TTTAAA TTT^AAA
Eam1105I 2642 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3595 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 565 GATATC GAT^ATC
FseI 22 GGCCGGCC GGCCGG^CC
HindIII 2996 AAGCTT A^AGCTT
KasI 2053 GGCGCC G^GCGCC
KpnI 2061 GGTACC GGTAC^C
MroI 1896 TCCGGA T^CCGGA
NarI 2053 GGCGCC GG^CGCC
PflFI 2936 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2636 CACGTG CAC^GTG
PmlI 2636 CACGTG CAC^GTG
Psp1406I 1231 AACGTT AA^CGTT
PvuII 456 CAGCTG CAG^CTG
SalI 2837 GTCGAC G^TCGAC
ScaI 2115 AGTACT AGT^ACT
SexAI 2679 ACCTGGT A^CC[AT]GGT
SfoI 2053 GGCGCC GGC^GCC
SmaI 2076 CCCGGG CCC^GGG
TfiI 1875 GAATC G^A[AT]TC
Tth111I 2936 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1537 TCTAGA T^CTAGA
XmaCI 2076 CCCGGG C^CCGGG
XmaI 2076 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Asp700I 1529 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3014 GAACACATTC
BamHI 823 GGATCC G^GATCC
949 GGATCC
BbvCI 2665 CCTCAGC CCTCAGC(-5/-2)
2228 GCTGAGG
BcgI 2050 GCAGGCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2346 GCAGAAGGTTCG
BclI 352 TGATCA T^GATCA
441 TGATCA
BsaAI 2162 TACGTG [CT]AC^GT[GA]
2636 CACGTG
BsaI 1824 GAGACC GGTCTC(1/5)
3648 GAGACC
BsaWI 1149 ACCGGA [AT]^CCGG[AT]
1896 TCCGGA
BsiEI 25 CGGCCG CG[GA][CT]^CG
111 CGGCCG
BspHI 2217 TCATGA T^CATGA
2511 TCATGA
BspMI 1678 ACCTGC ACCTGC(4/8)
901 GCAGGT
EagI 25 CGGCCG C^GGCCG
111 CGGCCG
EclXI 25 CGGCCG C^GGCCG
111 CGGCCG
Eco52I 25 CGGCCG C^GGCCG
111 CGGCCG
FbaI 352 TGATCA T^GATCA
441 TGATCA
NaeI 23 GCCGGC GCC^GGC
3312 GCCGGC
NcoI 155 CCATGG C^CATGG
2832 CCATGG
NgoMIV 23 GCCGGC G^CCGGC
3312 GCCGGC
NspI 1240 ACATGC [GA]CATG^[CT]
3667 ACATGC
PaeR7I 914 CTCGAG C^TCGAG
2630 CTCGAG
RcaI 2217 TCATGA T^CATGA
2511 TCATGA
SapI 2107 GCTCTTC GCTCTTC(1/4)
3588 GCTCTTC
StuI 388 AGGCCT AGG^CCT
3210 AGGCCT
XhoI 914 CTCGAG C^TCGAG
2630 CTCGAG
XmnI 1529 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3014 GAACACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII