##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BalI BanII BbeI BbrPI BcgI
BciVI BglI BglII BlnI BsaAI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI Bsp1407I BspDI BspEI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstZ17I Bsu36I
ClaI CpoI DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I FseI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SspBI
StuI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AciI 2048 GCGG CCGC(-3/-1)
AflII 1008 CTTAAG C^TTAAG
AflIII 2984 ACATGT A^C[GA][CT]GT
AspHI 1534 GTGCTC G[AT]GC[AT]^C
BbvCI 2254 CCTCAGC CCTCAGC(-5/-2)
BfrI 1008 CTTAAG C^TTAAG
BlpI 571 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 571 GCTTAGC GC^T[ACGT]AGC
BsiHKAI 1534 GTGCTC G[AT]GC[AT]^C
BsmBI 2747 GAGACG CGTCTC(1/5)
BsmI 281 GCATTC GAATGC(1/-1)
BspLU11I 2984 ACATGT A^CATGT
BspMI 455 ACCTGC ACCTGC(4/8)
BsrFI 2809 ACCGGC [GA]^CCGG[CT]
BstAPI 729 GCACAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1367 CCAGGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 571 GCTTAGC GC^T[ACGT]AGC
Cfr10I 2809 ACCGGC [GA]^CCGG[CT]
DraI 2962 TTTAAA TTT^AAA
DraIII 730 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1800 CCATGG C^C[GA][CT]GG
EcoRV 2728 GATATC GAT^ATC
EcoT14I 1800 CCATGG C^C[AT][AT]GG
EcoT22I 2541 ATGCAT ATGCA^T
FauI 2048 GCGGG CCCGC(4/6)
HgaI 2139 GCGTC GACGC(5/10)
HincII 636 GTCAAC GT[CT]^[GA]AC
HindII 636 GTCAAC GT[CT]^[GA]AC
MaeII 296 ACGT A^CGT
NcoI 1800 CCATGG C^CATGG
NdeI 1221 CATATG CA^TATG
NsiI 2541 ATGCAT ATGCA^T
PshAI 703 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1941 CTGCAG CTGCA^G
SexAI 1471 ACCTGGT A^CC[AT]GGT
SmaI 238 CCCGGG CCC^GGG
SphI 1663 GCATGC GCATG^C
SspI 1871 AATATT AAT^ATT
StyI 1800 CCATGG C^C[AT][AT]GG
XcmI 1723 CCATCCATCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 238 CCCGGG C^CCGGG
XmaI 238 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AlwNI 1881 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
2152 CAGAGTCTG
AseI 615 ATTAAT AT^TAAT
812 ATTAAT
AsnI 615 ATTAAT AT^TAAT
812 ATTAAT
AvaI 238 CCCGGG C^[CT]CG[GA]G
1969 CCCGAG
BamHI 485 GGATCC G^GATCC
1280 GGATCC
BanI 2374 GGCACC G^G[CT][GA]CC
2739 GGTGCC
BbvI 765 GCAGC GCAGC(8/12)
1943 GCAGC
BcnI 238 CCCGG CC^[GC]GG
239 CCGGG
BsaBI 363 GATACGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2724 GATAGATATC
BsmFI 331 GGGAC GGGAC(10/14)
2279 GGGAC
BsoBI 238 CCCGGG C^[CT]CG[GA]G
1969 CCCGAG
BspHI 158 TCATGA T^CATGA
1451 TCATGA
BstBI 841 TTCGAA TT^CGAA
1075 TTCGAA
Cac8I 725 GCAAGC GC[ACGT]^[ACGT]GC
1663 GCATGC
CfoI 900 GCGC GCG^C
2085 GCGC
DraII 598 AGGCCCT [GA]G^G[ACGT]CC[CT]
3010 AGGCCCT
EcoO109I 598 AGGCCCT [GA]G^G[ACGT]CC[CT]
3010 AGGCCCT
EcoRI 42 GAATTC G^AATTC
2926 GAATTC
Fnu4HI 765 GCAGC GC^[ACGT]GC
1943 GCAGC
HapII 239 CCGG C^CGG
2810 CCGG
HhaI 900 GCGC GCG^C
2085 GCGC
HinP1I 900 GCGC G^CGC
2085 GCGC
HpaII 239 CCGG C^CGG
2810 CCGG
ItaI 765 GCAGC GC^[ACGT]GC
1943 GCAGC
MamI 363 GATACGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2724 GATAGATATC
MspI 239 CCGG C^CGG
2810 CCGG
NciI 238 CCCGG CC^[GC]GG
239 CCGGG
NspV 841 TTCGAA TT^CGAA
1075 TTCGAA
PshBI 615 ATTAAT AT^TAAT
812 ATTAAT
RcaI 158 TCATGA T^CATGA
1451 TCATGA
SfuI 841 TTCGAA TT^CGAA
1075 TTCGAA
TseI 765 GCAGC G^C[AT]GC
1943 GCAGC
Tsp45I 320 GTCAC ^GT[GC]AC
1040 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AvaII BbsI BclI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EarI EcoRII
FbaI FokI HaeIII HinfI HphI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI