##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AccII AccIII AclI AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BcnI BglI BlnI
BsaBI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsoBI BspEI BspLU11I
BspMI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I CpoI DraII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV FauI FseI FspI HaeII
HapII HgaI HpaI HpaII KasI
KspI MamI MluI MroI MspI
MvnI NaeI NarI NciI NcoI
NgoMIV NotI NruI NspV PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SacI
SacII SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 1639 GACGTC GACGT^C
Acc65I 799 GGTACC G^GTACC
AcyI 1639 GACGTC G[GA]^CG[CT]C
AflIII 2524 ACGTGT A^C[GA][CT]GT
Asp718I 799 GGTACC G^GTACC
AspHI 838 GAGCAC G[AT]GC[AT]^C
AvaII 3611 GGTCC G^G[AT]CC
BalI 3568 TGGCCA TGG^CCA
BanI 799 GGTACC G^G[CT][GA]CC
BanII 2665 GGGCTC G[GA]GC[CT]^C
BlpI 1953 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1953 GCTGAGC GC^T[ACGT]AGC
BsaAI 856 TACGTG [CT]AC^GT[GA]
BsaHI 1639 GACGTC G[GA]^CG[CT]C
BsgI 674 CTGCAC GTGCAG(16/14)
BsiHKAI 838 GAGCAC G[AT]GC[AT]^C
BsmBI 1344 GAGACG CGTCTC(1/5)
Bsp1407I 1095 TGTACA T^GTACA
BspDI 1583 ATCGAT AT^CGAT
BspHI 1687 TCATGA T^CATGA
BsrBI 718 GAGCGG CCGCTC(-3/-3)
BsrGI 1095 TGTACA T^GTACA
BstXI 2978 CCAAAATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1953 GCTGAGC GC^T[ACGT]AGC
CfoI 2355 GCGC GCG^C
Cfr13I 3611 GGTCC G^G[ACGT]CC
ClaI 1583 ATCGAT AT^CGAT
DraIII 1620 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3917 GACAATTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 3568 TGGCCA [CT]^GGCC[GA]
EcoRI 2559 GAATTC G^AATTC
EcoT22I 3064 ATGCAT ATGCA^T
HaeIII 3569 GGCC GG^CC
HhaI 2355 GCGC GCG^C
Hin1I 1639 GACGTC G[GA]^CG[CT]C
HinP1I 2355 GCGC G^CGC
KpnI 799 GGTACC GGTAC^C
MfeI 3532 CAATTG C^AATTG
MluNI 3568 TGGCCA TGG^CCA
MscI 3568 TGGCCA TGG^CCA
MunI 3532 CAATTG C^AATTG
NheI 2888 GCTAGC G^CTAGC
NlaIV 799 GGTACC GG[ACGT]^[ACGT]CC
NsiI 3064 ATGCAT ATGCA^T
PacI 2697 TTAATTAA TTAAT^TAA
PflMI 2273 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 2685 GAGTC GAGTC(4/5)
PshAI 1875 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1478 CTGCAG CTGCA^G
RcaI 1687 TCATGA T^CATGA
SalI 1362 GTCGAC G^TCGAC
Sau96I 3611 GGTCC G^G[ACGT]CC
ScaI 4143 AGTACT AGT^ACT
SspBI 1095 TGTACA T^GTACA
Van91I 2273 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1320 CCAATCTTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 1362 GTCGAC GT^[AC][GT]AC
1872 GTAGAC
AciI 720 GCGG CCGC(-3/-1)
3444 GCGG
Asp700I 813 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3486 GAAAAACTTC
BbsI 1890 GAAGAC GAAGAC(2/6)
2687 GTCTTC
BciVI 478 GTATCC GTATCC(6/5)
1275 GTATCC
BclI 1012 TGATCA T^GATCA
3875 TGATCA
BmyI 838 GAGCAC G[AGT]GC[ACT]^C
2665 GGGCTC
BpmI 398 CTCCAG CTGGAG(16/14)
1391 CTCCAG
BpuAI 1890 GAAGAC GAAGAC(2/6)
2687 GTCTTC
Bsp1286I 838 GAGCAC G[AGT]GC[ACT]^C
2665 GGGCTC
BsrDI 980 CATTGC GCAATG(2/0)
3480 GCAATG
EcoT14I 1599 CCTTGG C^C[AT][AT]GG
2896 CCAAGG
FbaI 1012 TGATCA T^GATCA
3875 TGATCA
NdeI 421 CATATG CA^TATG
4047 CATATG
StyI 1599 CCTTGG C^C[AT][AT]GG
2896 CCAAGG
XmnI 813 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3486 GAAAAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AcsI AfaI AflII AluI AlwI
ApoI AseI AsnI BbvI BfaI
BfrI BglII BsaJI BsiYI BslI
BsmAI BsmFI BsmI BsrI BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII DraI EarI EcoRII Fnu4HI
FokI HincII HindII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NspI PshBI PvuII
RsaI Sau3AI ScrFI SfaNI SfcI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII