##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AgeI AhdI Aor51HI ApaI AscI
AspEI AspI AviII AvrII BbeI
BbvCI BcgI BciVI BglI BglII
BlnI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspEI
BspHI BspLU11I BspMI BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstXI BstZ17I CfoI Cfr10I
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI FspI HaeII HhaI HinP1I
HpaI KasI KspI MamI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NotI NruI PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 3647 GGTACC G^GTACC
AcyI 976 GACGCC G[GA]^CG[CT]C
AflII 1411 CTTAAG C^TTAAG
AflIII 3809 ACACGT A^C[GA][CT]GT
AocI 408 CCTGAGG CC^T[ACGT]AGG
Asp718I 3647 GGTACC G^GTACC
AvaI 1325 CCCGAG C^[CT]CG[GA]G
AvaII 1521 GGACC G^G[AT]CC
BalI 2054 TGGCCA TGG^CCA
BamHI 1753 GGATCC G^GATCC
BanI 3647 GGTACC G^G[CT][GA]CC
BbrPI 3810 CACGTG CAC^GTG
BbsI 3358 GAAGAC GAAGAC(2/6)
BclI 2418 TGATCA T^GATCA
BfrI 1411 CTTAAG C^TTAAG
BlpI 1426 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1426 GCTGAGC GC^T[ACGT]AGC
BpuAI 3358 GAAGAC GAAGAC(2/6)
BsaHI 976 GACGCC G[GA]^CG[CT]C
BseRI 1276 GAGGAG GAGGAG(10/8)
BsoBI 1325 CCCGAG C^[CT]CG[GA]G
BspDI 1255 ATCGAT AT^CGAT
BstAPI 1360 GCAAAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1091 TTCGAA TT^CGAA
BstYI 1753 GGATCC [GA]^GATC[CT]
Bsu36I 408 CCTGAGG CC^T[ACGT]AGG
CelII 1426 GCTGAGC GC^T[ACGT]AGC
ClaI 1255 ATCGAT AT^CGAT
DraII 2491 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 3264 CCATGG C^C[GA][CT]GG
EaeI 2054 TGGCCA [CT]^GGCC[GA]
Eco81I 408 CCTGAGG CC^T[ACGT]AGG
EcoNI 2348 CCTAAAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2491 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 2412 GAATTC G^AATTC
EcoT14I 3264 CCATGG C^C[AT][AT]GG
EcoT22I 1586 ATGCAT ATGCA^T
FbaI 2418 TGATCA T^GATCA
Hin1I 976 GACGCC G[GA]^CG[CT]C
KpnI 3647 GGTACC GGTAC^C
MflI 1753 GGATCC [GA]^GATC[CT]
MluNI 2054 TGGCCA TGG^CCA
MscI 2054 TGGCCA TGG^CCA
NcoI 3264 CCATGG C^CATGG
NheI 1119 GCTAGC G^CTAGC
NsiI 1586 ATGCAT ATGCA^T
NspV 1091 TTCGAA TT^CGAA
PmaCI 3810 CACGTG CAC^GTG
PmlI 3810 CACGTG CAC^GTG
SapI 1405 GAAGAGC GCTCTTC(1/4)
ScaI 1300 AGTACT AGT^ACT
SfuI 1091 TTCGAA TT^CGAA
SphI 313 GCATGC GCATG^C
StuI 158 AGGCCT AGG^CCT
StyI 3264 CCATGG C^C[AT][AT]GG
XbaI 2106 TCTAGA T^CTAGA
XhoII 1753 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 1049 GTGCAC G^TGCAC
2695 GTGCAC
AlwI 1754 GATCC GGATC(4/5)
1753 GGATC
AlwNI 1451 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
3468 CAGTCACTG
ApaLI 1049 GTGCAC G^TGCAC
2695 GTGCAC
AseI 3001 ATTAAT AT^TAAT
3184 ATTAAT
AsnI 3001 ATTAAT AT^TAAT
3184 ATTAAT
Asp700I 446 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2788 GAAGTATTTC
BanII 700 GAGCTC G[GA]GC[CT]^C
1927 GAGCTC
BpmI 703 CTCCAG CTGGAG(16/14)
3778 CTGGAG
BsaAI 2041 TACGTG [CT]AC^GT[GA]
3810 CACGTG
BsmAI 388 GAGAC GTCTC(1/5)
2608 GAGAC
BsrDI 1389 CATTGC GCAATG(2/0)
2206 CATTGC
HgaI 976 GACGC GACGC(5/10)
1453 GCGTC
MfeI 1237 CAATTG C^AATTG
2855 CAATTG
MunI 1237 CAATTG C^AATTG
2855 CAATTG
NspI 313 GCATGC [GA]CATG^[CT]
2980 GCATGT
PshBI 3001 ATTAAT AT^TAAT
3184 ATTAAT
PvuII 1784 CAGCTG CAG^CTG
2194 CAGCTG
SacI 700 GAGCTC GAGCT^C
1927 GAGCTC
XcmI 2470 CCAATACATACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2581 CCACTGAGCTATTGG
XmnI 446 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2788 GAAGTATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BbvI BcnI BfaI BmyI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmFI BsmI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I