##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AhdI        AocI        AscI        AseI        AsnI        
AspEI       AviII       AvrII       BbeI        BbrPI       
BfrI        BlnI        BseAI       BsiWI       BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BssHII      
Bst1107I    BstBI       BstZ17I     Bsu36I      ClaI        
CpoI        Eam1105I    Eco81I      EcoRI       EcoRV       
FspI        HindIII     HpaI        KasI        MfeI        
MluI        MroI        MunI        NarI        NheI        
NotI        NspV        PacI        PaeR7I      PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SalI        ScaI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SwaI        
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AflIII       4155  ACGTGT               A^C[GA][CT]GT
AgeI         3651  ACCGGT               A^CCGGT
Alw44I       3064  GTGCAC               G^TGCAC
Aor51HI       236  AGCGCT               AGC^GCT
ApaLI        3064  GTGCAC               G^TGCAC
AspI         4424  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         259  GGATCC               G^GATCC
BcgI          165  GCAGTCCCGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3712  GGATAC               GTATCC(6/5)
BglI         1894  GCCCCCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI         852  GATGCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        3651  ACCGGT               [AT]^CCGG[AT]
BsmBI        1440  CGTCTC               CGTCTC(1/5)
BspHI        4094  TCATGA               T^CATGA
BstEII       2681  GGTCACC              G^GT[ACGT]ACC
BstPI        2681  GGTCACC              G^GT[ACGT]ACC
BstXI        2806  CCAACCTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI         1349  GACACTGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          394  CGGCCG               C^GGCCG
EclXI         394  CGGCCG               C^GGCCG
Eco47III      236  AGCGCT               AGC^GCT
Eco52I        394  CGGCCG               C^GGCCG
EcoO65I      2681  GGTCACC              G^GT[ACGT]ACC
EcoT22I      3531  ATGCAT               ATGCA^T
FseI          395  GGCCGGCC             GGCCGG^CC
MamI          852  GATGCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NruI          226  TCGCGA               TCG^CGA
NsiI         3531  ATGCAT               ATGCA^T
PflFI        4424  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        3651  ACCGGT               A^CCGGT
RcaI         4094  TCATGA               T^CATGA
SbfI         1760  CCTGCAGG             CCTGCA^GG
SfiI         1893  GGCCCCCTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I     1760  CCTGCAGG             CCTGCA^GG
SspI          710  AATATT               AAT^ATT
StuI         2161  AGGCCT               AGG^CCT
Tth111I      4424  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1318  CCATGGAGGACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

Acc65I       3155  GGTACC               G^GTACC
             3850  GGTACC              
AcyI          149  GGCGTC               G[GA]^CG[CT]C
             1224  GACGCC              
Asp700I       587  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2894  GAATCGGTTC          
Asp718I      3155  GGTACC               G^GTACC
             3850  GGTACC              
BalI         1315  TGGCCA               TGG^CCA
             2815  TGGCCA              
BbvCI        2364  CCTCAGC              CCTCAGC(-5/-2)
             2822  CCTCAGC             
BclI         1379  TGATCA               T^GATCA
             1552  TGATCA              
BglII        1192  AGATCT               A^GATCT
             1706  AGATCT              
BlpI         2714  GCTCAGC              GC^T[ACGT]AGC
             4175  GCTCAGC             
Bpu1102I     2714  GCTCAGC              GC^T[ACGT]AGC
             4175  GCTCAGC             
BsaAI        1023  TACGTG               [CT]AC^GT[GA]
             4154  TACGTG              
BsaHI         149  GGCGTC               G[GA]^CG[CT]C
             1224  GACGCC              
BsaI          176  GGTCTC               GGTCTC(1/5)
              222  GGTCTC              
BsiEI         394  CGGCCG               CG[GA][CT]^CG
              467  CGACCG              
Bsp1407I     3938  TGTACA               T^GTACA
             4249  TGTACA              
BsrGI        3938  TGTACA               T^GTACA
             4249  TGTACA              
BssSI        3560  CTCGTG               CACGAG(-5/-1)
             3969  CTCGTG              
CelII        2714  GCTCAGC              GC^T[ACGT]AGC
             4175  GCTCAGC             
DraI          827  TTTAAA               TTT^AAA
             4130  TTTAAA              
DraIII        720  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1665  CACACAGTG           
FbaI         1379  TGATCA               T^GATCA
             1552  TGATCA              
Hin1I         149  GGCGTC               G[GA]^CG[CT]C
             1224  GACGCC              
HincII       1740  GTCAAC               GT[CT]^[GA]AC
             2706  GTTGAC              
HindII       1740  GTCAAC               GT[CT]^[GA]AC
             2706  GTTGAC              
KpnI         3155  GGTACC               GGTAC^C
             3850  GGTACC              
MluNI        1315  TGGCCA               TGG^CCA
             2815  TGGCCA              
MscI         1315  TGGCCA               TGG^CCA
             2815  TGGCCA              
NaeI          396  GCCGGC               GCC^GGC
             3521  GCCGGC              
NdeI         1149  CATATG               CA^TATG
             2295  CATATG              
NgoMIV        396  GCCGGC               G^CCGGC
             3521  GCCGGC              
SapI         1617  GAAGAGC              GCTCTTC(1/4)
              199  GCTCTTC             
SmaI         2959  CCCGGG               CCC^GGG
             3889  CCCGGG              
SmlI         1833  CTTGAG               C^T[CT][GA]AG
             3644  CTTGAG              
SphI         1459  GCATGC               GCATG^C
             1940  GCATGC              
SspBI        3938  TGTACA               T^GTACA
             4249  TGTACA              
XmaCI        2959  CCCGGG               C^CCGGG
             3889  CCCGGG              
XmaI         2959  CCCGGG               C^CCGGG
             3889  CCCGGG              
XmnI          587  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2894  GAATCGGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApaI        ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacI        SacII       Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII