##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AflII AgeI Aor51HI
AscI AseI AsnI Asp718I AvrII
BamHI BcgI BclI BfrI BglII
BlnI BsaBI BsiWI Bsp1407I BspDI
BsrDI BsrGI BssHII BstBI ClaI
CpoI DraI DraIII DrdI Eco47III
EcoRV FbaI FseI HindIII HpaI
KpnI MamI MfeI MluI MunI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshBI
RsrII SalI SapI SfuI SnaBI
SpeI SspBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 565 TCCGGA T^CCGGA
AclI 1255 AACGTT AA^CGTT
AhdI 641 GACATGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 641 GACATGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2177 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 3267 GTATCC GTATCC(6/5)
BsaI 2412 GGTCTC GGTCTC(1/5)
BsaWI 565 TCCGGA [AT]^CCGG[AT]
BseAI 565 TCCGGA T^CCGGA
BspEI 565 TCCGGA T^CCGGA
BspHI 1284 TCATGA T^CATGA
BspMI 2423 GCAGGT ACCTGC(4/8)
BssSI 1811 CACGAG CACGAG(-5/-1)
Bst1107I 772 GTATAC GTA^TAC
BstAPI 3682 GCAGATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 772 GTATAC GTA^TAC
EagI 1962 CGGCCG C^GGCCG
Eam1105I 641 GACATGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1962 CGGCCG C^GGCCG
Eco52I 1962 CGGCCG C^GGCCG
KspI 436 CCGCGG CCGC^GG
MroI 565 TCCGGA T^CCGGA
MseI 3868 TTAA T^TAA
PflFI 2177 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1255 AACGTT AA^CGTT
PstI 715 CTGCAG CTGCA^G
PvuI 1357 CGATCG CGAT^CG
RcaI 1284 TCATGA T^CATGA
SacII 436 CCGCGG CCGC^GG
SbfI 714 CCTGCAGG CCTGCA^GG
ScaI 1983 AGTACT AGT^ACT
SexAI 2408 ACCTGGT A^CC[AT]GGT
SgrAI 93 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 3498 CTTGAG C^T[CT][GA]AG
SphI 3691 GCATGC GCATG^C
Sse8387I 714 CCTGCAGG CCTGCA^GG
Tru9I 3868 TTAA T^TAA
Tth111I 2177 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3065 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 772 GTATAC GT^[AC][GT]AC
2813 GTCTAC
AflIII 738 ACATGT A^C[GA][CT]GT
2907 ACATGT
Alw44I 1567 GTGCAC G^TGCAC
2911 GTGCAC
AocI 635 CCTGAGG CC^T[ACGT]AGG
3436 CCTGAGG
ApaLI 1567 GTGCAC G^TGCAC
2911 GTGCAC
Asp700I 1195 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3145 GAATCCCTTC
AviII 855 TGCGCA TGC^GCA
1893 TGCGCA
BalI 1617 TGGCCA TGG^CCA
1947 TGGCCA
BbrPI 1943 CACGTG CAC^GTG
2228 CACGTG
BbvCI 3721 CCTCAGC CCTCAGC(-5/-2)
1130 GCTGAGG
BsiEI 1357 CGATCG CG[GA][CT]^CG
1962 CGGCCG
BspLU11I 738 ACATGT A^CATGT
2907 ACATGT
BsrFI 94 GCCGGC [GA]^CCGG[CT]
150 GCCGGC
BstEII 1936 GGTCACC G^GT[ACGT]ACC
3520 GGTCACC
BstPI 1936 GGTCACC G^GT[ACGT]ACC
3520 GGTCACC
Bsu36I 635 CCTGAGG CC^T[ACGT]AGG
3436 CCTGAGG
Cfr10I 94 GCCGGC [GA]^CCGG[CT]
150 GCCGGC
Eco81I 635 CCTGAGG CC^T[ACGT]AGG
3436 CCTGAGG
EcoO65I 1936 GGTCACC G^GT[ACGT]ACC
3520 GGTCACC
EcoRI 1048 GAATTC G^AATTC
1522 GAATTC
EcoT22I 3689 ATGCAT ATGCA^T
3794 ATGCAT
FspI 855 TGCGCA TGC^GCA
1893 TGCGCA
MluNI 1617 TGGCCA TGG^CCA
1947 TGGCCA
MscI 1617 TGGCCA TGG^CCA
1947 TGGCCA
NaeI 94 GCCGGC GCC^GGC
150 GCCGGC
NgoMIV 94 GCCGGC G^CCGGC
150 GCCGGC
NsiI 3689 ATGCAT ATGCA^T
3794 ATGCAT
PmaCI 1943 CACGTG CAC^GTG
2228 CACGTG
PmlI 1943 CACGTG CAC^GTG
2228 CACGTG
SfiI 2242 GGCCCGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2489 GGCCTGCCCGGCC
TfiI 579 GAATC G^A[AT]TC
3145 GAATC
XmnI 1195 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3145 GAATCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshAI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI StuI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI