##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AfaI AgeI AhdI AocI ApaI
AscI AseI AsnI Asp718I AspEI
AviII BanI BbeI BbrPI BcgI
BciVI BclI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsiWI Bsp1407I BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I CpoI DraIII EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI Hin1I
KasI KpnI KspI MamI MfeI
MluI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacI SacII SalI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccII 486 CGCG CG^CG
Alw44I 2868 GTGCAC G^TGCAC
Aor51HI 2998 AGCGCT AGC^GCT
ApaLI 2868 GTGCAC G^TGCAC
AvrII 2095 CCTAGG C^CTAGG
BamHI 93 GGATCC G^GATCC
BglI 2987 GCCACGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2825 AGATCT A^GATCT
BlnI 2095 CCTAGG C^CTAGG
BsiEI 2967 CGGTCG CG[GA][CT]^CG
BsmBI 2123 CGTCTC CGTCTC(1/5)
BspDI 1340 ATCGAT AT^CGAT
BspHI 842 TCATGA T^CATGA
BspMI 1623 GCAGGT ACCTGC(4/8)
BsrBI 2540 GAGCGG CCGCTC(-3/-3)
BsrDI 2939 GCAATG GCAATG(2/0)
BstUI 486 CGCG CG^CG
ClaI 1340 ATCGAT AT^CGAT
Eco47III 2998 AGCGCT AGC^GCT
EcoRI 1653 GAATTC G^AATTC
HaeII 2998 AGCGCT [GA]GCGC^[CT]
HgaI 2514 GACGC GACGC(5/10)
HpaI 2282 GTTAAC GTT^AAC
MvnI 486 CGCG CG^CG
PflMI 768 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 842 TCATGA T^CATGA
SapI 2176 GCTCTTC GCTCTTC(1/4)
SbfI 3069 CCTGCAGG CCTGCA^GG
SmaI 2885 CCCGGG CCC^GGG
Sse8387I 3069 CCTGCAGG CCTGCA^GG
SspI 3871 AATATT AAT^ATT
StuI 2493 AGGCCT AGG^CCT
Tsp45I 248 GTCAC ^GT[GC]AC
Van91I 768 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1075 CCACTCGAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2885 CCCGGG C^CCGGG
XmaI 2885 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 2345 TCCGGA T^CCGGA
2549 TCCGGA
AflII 308 CTTAAG C^TTAAG
3589 CTTAAG
AspHI 909 GTGCTC G[AT]GC[AT]^C
2868 GTGCAC
AspI 1024 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1101 GACGAGGTC
BanII 425 GGGCTC G[GA]GC[CT]^C
3965 GAGCCC
BbsI 2607 GAAGAC GAAGAC(2/6)
2713 GTCTTC
BfrI 308 CTTAAG C^TTAAG
3589 CTTAAG
BpuAI 2607 GAAGAC GAAGAC(2/6)
2713 GTCTTC
BsaWI 2345 TCCGGA [AT]^CCGG[AT]
2549 TCCGGA
BseAI 2345 TCCGGA T^CCGGA
2549 TCCGGA
BseRI 1386 CTCCTC GAGGAG(10/8)
2484 GAGGAG
BsiHKAI 909 GTGCTC G[AT]GC[AT]^C
2868 GTGCAC
BsmI 3205 GAATGC GAATGC(1/-1)
2792 GCATTC
BspEI 2345 TCCGGA T^CCGGA
2549 TCCGGA
BssSI 660 CACGAG CACGAG(-5/-1)
1293 CTCGTG
CfoI 487 GCGC GCG^C
2999 GCGC
DraI 1638 TTTAAA TTT^AAA
3355 TTTAAA
DrdI 360 GACCATCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
752 GACAAAAATGTC
EcoNI 227 CCTGAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2090 CCTTGCCTAGG
FauI 2618 CCCGC CCCGC(4/6)
2542 GCGGG
HhaI 487 GCGC GCG^C
2999 GCGC
HinP1I 487 GCGC G^CGC
2999 GCGC
MroI 2345 TCCGGA T^CCGGA
2549 TCCGGA
NcoI 3020 CCATGG C^CATGG
3125 CCATGG
NdeI 1937 CATATG CA^TATG
3179 CATATG
PaeR7I 1078 CTCGAG C^TCGAG
3254 CTCGAG
PflFI 1024 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1101 GACGAGGTC
SexAI 2531 ACCTGGT A^CC[AT]GGT
2708 ACCTGGT
Tth111I 1024 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1101 GACGAGGTC
XhoI 1078 CTCGAG C^TCGAG
3254 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AflIII AluI AlwI
AlwNI ApoI Asp700I AvaI AvaII
BalI BbvCI BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII
XmnI