##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI Alw44I Aor51HI
ApaLI AscI Asp718I AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BspEI BsrBI BssHII Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
CpoI DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I FseI FspI
HincII HindII HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MvnI NarI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I StuI SwaI
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 606 GGCGTC G[GA]^CG[CT]C
AhdI 2506 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1625 CCTGAGG CC^T[ACGT]AGG
ApaI 1293 GGGCCC GGGCC^C
AseI 452 ATTAAT AT^TAAT
AsnI 452 ATTAAT AT^TAAT
AspEI 2506 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 875 GAGCTC G[AT]GC[AT]^C
AspI 3936 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 291 CCCGAG C^[CT]CG[GA]G
BciVI 4538 GTATCC GTATCC(6/5)
BcnI 2314 CCGGG CC^[GC]GG
BlpI 872 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 872 GCTGAGC GC^T[ACGT]AGC
BsaHI 606 GGCGTC G[GA]^CG[CT]C
BsiHKAI 875 GAGCTC G[AT]GC[AT]^C
BsmBI 608 CGTCTC CGTCTC(1/5)
BsoBI 291 CCCGAG C^[CT]CG[GA]G
Bsp1407I 3066 TGTACA T^GTACA
BspDI 1634 ATCGAT AT^CGAT
BsrGI 3066 TGTACA T^GTACA
BssSI 582 CACGAG CACGAG(-5/-1)
Bsu36I 1625 CCTGAGG CC^T[ACGT]AGG
CelII 872 GCTGAGC GC^T[ACGT]AGC
ClaI 1634 ATCGAT AT^CGAT
Eam1105I 2506 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1625 CCTGAGG CC^T[ACGT]AGG
EcoNI 981 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 577 GAATTC G^AATTC
EcoRV 4167 GATATC GAT^ATC
FauI 1255 CCCGC CCCGC(4/6)
Hin1I 606 GGCGTC G[GA]^CG[CT]C
MfeI 3489 CAATTG C^AATTG
MunI 3489 CAATTG C^AATTG
NaeI 2330 GCCGGC GCC^GGC
NciI 2314 CCGGG CC^[GC]GG
NgoMIV 2330 GCCGGC G^CCGGC
PflFI 3936 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3797 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 452 ATTAAT AT^TAAT
PstI 2067 CTGCAG CTGCA^G
SacI 875 GAGCTC GAGCT^C
SexAI 1277 ACCTGGT A^CC[AT]GGT
SmlI 2097 CTCAAG C^T[CT][GA]AG
SspBI 3066 TGTACA T^GTACA
Tth111I 3936 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Asp700I 380 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3657 GAATTGTTTC
BpmI 897 CTCCAG CTGGAG(16/14)
126 CTGGAG
BsgI 938 GTGCAG GTGCAG(16/14)
1025 GTGCAG
BspHI 2822 TCATGA T^CATGA
3539 TCATGA
BsrFI 1815 GCCGGT [GA]^CCGG[CT]
2330 GCCGGC
BstEII 1909 GGTTACC G^GT[ACGT]ACC
2802 GGTTACC
BstPI 1909 GGTTACC G^GT[ACGT]ACC
2802 GGTTACC
CfoI 2334 GCGC GCG^C
2349 GCGC
Cfr10I 1815 GCCGGT [GA]^CCGG[CT]
2330 GCCGGC
EcoO65I 1909 GGTTACC G^GT[ACGT]ACC
2802 GGTTACC
EcoT22I 565 ATGCAT ATGCA^T
2840 ATGCAT
HaeII 2333 GGCGCT [GA]GCGC^[CT]
2348 AGCGCC
HhaI 2334 GCGC GCG^C
2349 GCGC
HinP1I 2334 GCGC G^CGC
2349 GCGC
HindIII 3562 AAGCTT A^AGCTT
3683 AAGCTT
NcoI 1676 CCATGG C^CATGG
2460 CCATGG
NsiI 565 ATGCAT ATGCA^T
2840 ATGCAT
PvuII 1379 CAGCTG CAG^CTG
2553 CAGCTG
RcaI 2822 TCATGA T^CATGA
3539 TCATGA
SapI 2075 GAAGAGC GCTCTTC(1/4)
2284 GAAGAGC
ScaI 2530 AGTACT AGT^ACT
2731 AGTACT
SpeI 3327 ACTAGT A^CTAGT
4301 ACTAGT
XmnI 380 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3657 GAATTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AvaII BamHI
BanI BanII BbsI BbvI BclI
BfaI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspLU11I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeI
NdeII NlaIII NlaIV NspI PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII