##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI Aor51HI ApaI AscI
Asp718I AvaI AviII BalI BanI
BbeI BbrPI BclI BfrI BglI
BlpI Bpu1102I BsaAI BsaHI BseAI
BsiEI BsiWI BsoBI Bsp1407I BspEI
BsrGI BssHII BstEII BstPI BstUI
BstXI CelII CfoI CpoI DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII KasI KpnI KspI MluI
MluNI MroI MscI MvnI NaeI
NarI NgoMIV NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccI 3081 GTATAC GT^[AC][GT]AC
AclI 1828 AACGTT AA^CGTT
AflIII 3050 ACATGT A^C[GA][CT]GT
AhdI 2777 GACTACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1678 GTGCAC G^TGCAC
AocI 516 CCTAAGG CC^T[ACGT]AGG
ApaLI 1678 GTGCAC G^TGCAC
AseI 3171 ATTAAT AT^TAAT
AsnI 3171 ATTAAT AT^TAAT
Asp700I 1974 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2777 GACTACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1622 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1524 CCTAGG C^CTAGG
BcgI 2663 GCAGAGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 976 GTATCC GTATCC(6/5)
BcnI 354 CCGGG CC^[GC]GG
BglII 2713 AGATCT A^GATCT
BlnI 1524 CCTAGG C^CTAGG
BsaBI 2670 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2070 ACCGGA [AT]^CCGG[AT]
BsgI 2661 GTGCAG GTGCAG(16/14)
BsmBI 196 CGTCTC CGTCTC(1/5)
BspDI 365 ATCGAT AT^CGAT
BspLU11I 3050 ACATGT A^CATGT
BspMI 903 ACCTGC ACCTGC(4/8)
BsrBI 4052 GAGCGG CCGCTC(-3/-3)
BsrFI 2524 ACCGGC [GA]^CCGG[CT]
BssSI 2792 CTCGTG CACGAG(-5/-1)
Bst1107I 3081 GTATAC GTA^TAC
BstBI 334 TTCGAA TT^CGAA
BstZ17I 3081 GTATAC GTA^TAC
Bsu36I 516 CCTAAGG CC^T[ACGT]AGG
Cfr10I 2524 ACCGGC [GA]^CCGG[CT]
ClaI 365 ATCGAT AT^CGAT
Eam1105I 2777 GACTACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 516 CCTAAGG CC^T[ACGT]AGG
EcoT22I 2407 ATGCAT ATGCA^T
FauI 2897 GCGGG CCCGC(4/6)
HgaI 1015 GACGC GACGC(5/10)
HincII 77 GTTAAC GT[CT]^[GA]AC
HindII 77 GTTAAC GT[CT]^[GA]AC
HpaI 77 GTTAAC GTT^AAC
MamI 2670 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3723 CAATTG C^AATTG
MunI 3723 CAATTG C^AATTG
NciI 354 CCGGG CC^[GC]GG
NdeI 1683 CATATG CA^TATG
NheI 3767 GCTAGC G^CTAGC
NsiI 2407 ATGCAT ATGCA^T
NspV 334 TTCGAA TT^CGAA
PflFI 1622 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3487 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3171 ATTAAT AT^TAAT
Psp1406I 1828 AACGTT AA^CGTT
SacI 1781 GAGCTC GAGCT^C
ScaI 3876 AGTACT AGT^ACT
SfuI 334 TTCGAA TT^CGAA
SphI 2821 GCATGC GCATG^C
SspI 3713 AATATT AAT^ATT
StuI 2788 AGGCCT AGG^CCT
Tth111I 1622 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3487 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1974 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BamHI 681 GGATCC G^GATCC
2156 GGATCC
BanII 1781 GAGCTC G[GA]GC[CT]^C
4174 GAGCCC
BsaI 1059 GAGACC GGTCTC(1/5)
2983 GGTCTC
BseRI 1408 CTCCTC GAGGAG(10/8)
3413 GAGGAG
BsrDI 1635 CATTGC GCAATG(2/0)
4407 CATTGC
DraI 3694 TTTAAA TTT^AAA
4333 TTTAAA
DraII 237 AGGACCT [GA]G^G[ACGT]CC[CT]
1528 GGGTCCT
DsaI 981 CCATGG C^C[GA][CT]GG
3655 CCATGG
EcoO109I 237 AGGACCT [GA]G^G[ACGT]CC[CT]
1528 GGGTCCT
NcoI 981 CCATGG C^CATGG
3655 CCATGG
PpuMI 237 AGGACCT [GA]G^G[AT]CC[CT]
1528 GGGTCCT
XbaI 152 TCTAGA T^CTAGA
1695 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BbsI
BbvCI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRI EcoRII EcoRV EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII