##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccII AcyI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AspEI
AspI AvaI AvrII BbeI BbrPI
BbvCI BcgI BciVI BcnI BglI
BlnI BsaAI BsaHI BsaI BsiEI
BsiWI BsmBI BsoBI BspDI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI DraII DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
FseI HaeII Hin1I KasI KspI
MluI MslI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI PshAI
PvuI RsrII SacI SacII SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I Tth111I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccIII 526 TCCGGA T^CCGGA
AclI 1449 AACGTT AA^CGTT
AflII 715 CTTAAG C^TTAAG
AflIII 4266 ACATGT A^C[GA][CT]GT
AgeI 1150 ACCGGT A^CCGGT
AocI 2340 CCTTAGG CC^T[ACGT]AGG
AseI 4042 ATTAAT AT^TAAT
AsnI 4042 ATTAAT AT^TAAT
AviII 2423 TGCGCA TGC^GCA
BalI 663 TGGCCA TGG^CCA
BanII 761 GAGCCC G[GA]GC[CT]^C
BfrI 715 CTTAAG C^TTAAG
BsaBI 3844 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 526 TCCGGA T^CCGGA
BsgI 2813 GTGCAG GTGCAG(16/14)
BspEI 526 TCCGGA T^CCGGA
BspLU11I 4266 ACATGT A^CATGT
BsrFI 1150 ACCGGT [GA]^CCGG[CT]
BssKI 2501 CCAGG ^CC[ACGT]GG
BstNI 2501 CCAGG CC^[AT]GG
Bsu36I 2340 CCTTAGG CC^T[ACGT]AGG
CfoI 2424 GCGC GCG^C
Cfr10I 1150 ACCGGT [GA]^CCGG[CT]
DrdI 505 GACTCTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 663 TGGCCA [CT]^GGCC[GA]
EarI 3171 GAAGAG CTCTTC(1/4)
Eco81I 2340 CCTTAGG CC^T[ACGT]AGG
EcoRI 3407 GAATTC G^AATTC
EcoRII 2501 CCAGG ^CC[AT]GG
EcoT22I 166 ATGCAT ATGCA^T
FauI 2917 GCGGG CCCGC(4/6)
FspI 2423 TGCGCA TGC^GCA
HgaI 809 GACGC GACGC(5/10)
HhaI 2424 GCGC GCG^C
HinP1I 2424 GCGC G^CGC
HindIII 1284 AAGCTT A^AGCTT
HpaI 2827 GTTAAC GTT^AAC
Ksp632I 3171 GAAGAG CTCTTC(1/4)
MaeII 1450 ACGT A^CGT
MamI 3844 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 667 CAATTG C^AATTG
MluNI 663 TGGCCA TGG^CCA
MroI 526 TCCGGA T^CCGGA
MscI 663 TGGCCA TGG^CCA
MunI 667 CAATTG C^AATTG
MvaI 2501 CCAGG CC^[AT]GG
NsiI 166 ATGCAT ATGCA^T
PflMI 4117 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1150 ACCGGT A^CCGGT
PshBI 4042 ATTAAT AT^TAAT
Psp1406I 1449 AACGTT AA^CGTT
SalI 502 GTCGAC G^TCGAC
ScrFI 2501 CCAGG CC^[ACGT]GG
SpeI 3183 ACTAGT A^CTAGT
SphI 2696 GCATGC GCATG^C
StuI 2078 AGGCCT AGG^CCT
SwaI 3845 ATTTAAAT ATTT^AAAT
Van91I 4117 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 1199 GGTACC G^GTACC
1519 GGTACC
AccI 502 GTCGAC GT^[AC][GT]AC
1220 GTCTAC
AlwNI 2704 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
3121 CAGTTTCTG
Asp718I 1199 GGTACC G^GTACC
1519 GGTACC
AspHI 201 GAGCAC G[AT]GC[AT]^C
4112 GAGCAC
AvaII 2067 GGTCC G^G[AT]CC
2601 GGACC
BamHI 321 GGATCC G^GATCC
2463 GGATCC
BbsI 2533 GAAGAC GAAGAC(2/6)
637 GTCTTC
BglII 883 AGATCT A^GATCT
1358 AGATCT
BlpI 1395 GCTGAGC GC^T[ACGT]AGC
2573 GCTTAGC
BpmI 788 CTGGAG CTGGAG(16/14)
968 CTGGAG
Bpu1102I 1395 GCTGAGC GC^T[ACGT]AGC
2573 GCTTAGC
BpuAI 2533 GAAGAC GAAGAC(2/6)
637 GTCTTC
BsaWI 526 TCCGGA [AT]^CCGG[AT]
1150 ACCGGT
BsiHKAI 201 GAGCAC G[AT]GC[AT]^C
4112 GAGCAC
BsmI 1545 GAATGC GAATGC(1/-1)
3152 GCATTC
BspHI 1005 TCATGA T^CATGA
2447 TCATGA
BspMI 3975 ACCTGC ACCTGC(4/8)
1605 GCAGGT
BstXI 1308 CCAGAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2550 CCATTTGATTGG
CelII 1395 GCTGAGC GC^T[ACGT]AGC
2573 GCTTAGC
DsaI 2100 CCATGG C^C[GA][CT]GG
3533 CCATGG
EcoNI 1096 CCTTCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1770 CCTAGAAAAGG
HapII 527 CCGG C^CGG
1151 CCGG
HpaII 527 CCGG C^CGG
1151 CCGG
KpnI 1199 GGTACC GGTAC^C
1519 GGTACC
MspI 527 CCGG C^CGG
1151 CCGG
NcoI 2100 CCATGG C^CATGG
3533 CCATGG
PstI 3977 CTGCAG CTGCA^G
4153 CTGCAG
RcaI 1005 TCATGA T^CATGA
2447 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I BanI BbvI BclI
BfaI BmyI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BstF5I BstYI
Cac8I Cfr13I DdeI DpnII DraI
EcoRV EcoT14I FbaI Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MwoI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScaI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI